Microbiome sequence analysis of the UK Crop Microbiome CryoBank resource

Activity: Talk, evidence or presentation typesOral presentation


The UK Crop Microbiome CryoBank is a BBSRC-funded Bioinformatics and Biological Resource of cryopreserved andcharacterisedcrop microbiomes, which aims to underpin national and international crop research. Material is from seven major UK crops: (barley, oats, oil seed rape, potato, sugar beet, vining peas and wheat) grown in three different soil types from regions across the UK. The resource comprises preserved microbiomes and culturable isolates focused on crop rhizospheres; and sequence data for the microbiomes and isolate genome sequences; plus associated metadata for the crop and soils. The complete resource for the ongoing project is discoverable via an online database: AgMicrobiomeBase [https://agmicrobiomebase.org/]. Crop and soil microbiomes are being sequenced for taxonomic groups of by metabarcoding for bacterial (16S) and fungal communities (ITS, selected samples) and overall community structure by shotgun metagenome sequencing. Culturable isolates are being whole genome sequenced by de novo sequencing. Sequence data analysis pipelines are being optimised for protocol standardisation and to provide the optimal state-of-the-art data outputs. Active involvement with EBI MGNify will serve to improve microbiome datasets utility. One of the key aims of the project is to provide legacy so that future analyses can be applied to the datasets using updated tools, for advanced analyses. The main sequence data analysis aims are to (i) provide an accessible dataset; (ii) comparisons of the soil and crop sample type communities; (iii) standardised protocols for use with inherently complex microbiota communities. The datasets and resources are openly accessible for academic, policy and industry interests.
Period7 Apr 2022
Event titleThe Microbiology Society Annual Conference 2022
Event typeConference
LocationBelfast , United KingdomShow on map