Microbiome sequence data analysis of the UK Crop Microbiome CryoBank

Activity: Talk, evidence or presentation typesOral presentation

Description

The UK Crop Microbiome CryoBank Resource intends to underpin national and international crop research on seven major UK crops: (barley, oats, oil seed rape, potato, sugar beet, vining peas and wheat) grown in three different soil types in
different regions. The resource consists of preserved microbiomes and culturable isolates focused on crop rhizospheres and genome sequences with providing associated metadata. The complete resource for the ongoing project is discoverable via an online database: https://agmicrobiomebase.org/.

Crop and soil microbiomes are being sequenced for taxonomic groups on bacterial (16S) and fungal communities (ITS - selected samples), and overall community structure by shotgun metagenome sequencing. Culturable isolates are being whole genome sequenced by de novo sequencing. Sequence data analysis pipelines are being optimised for protocol standardisation and to provide the optimal state-ofthe-art data outputs. Active involvement with EBI MGNify will serve to improve microbiome datasets utility.

The main sequence data analysis aims are to (I) provide an accessible dataset; (II) comparisons of the soil and crop sample type communities; (III) standardised protocols for use with inherently complex microbiota communities. The datasets and resources are openly accessible to provide legacy so that future analyses can be applied for academic, policy and industry interests.
Period26 May 2022
Event title3rd Plant Microbiome Symposium 2022
Event typeConference
LocationDundee, United Kingdom