A comparison of single-coverage and multi-coverage metagenomic binning reveals extensive hidden contamination

J. Mattock, M. Watson

Research output: Contribution to journalShort communication peer-review

2 Citations (Scopus)
47 Downloads (Pure)

Abstract

Metagenomic binning has revolutionized the study of uncultured microorganisms. Here we compare single- and multi-coverage binning on the same set of samples, and demonstrate that multi-coverage binning produces better results than single-coverage binning and identifies contaminant contigs and chimeric bins that other approaches miss. While resource expensive, multi-coverage binning is a superior approach and should always be performed over single-coverage binning.
Original languageEnglish
Pages (from-to)1170-1173
Number of pages4
JournalNature Methods
Volume20
Issue number8
Early online date29 Jun 2023
DOIs
Publication statusPrint publication - Aug 2023
Externally publishedYes

Bibliographical note

© 2023. The Author(s), under exclusive licence to Springer Nature America, Inc.

Keywords

  • Algorithms
  • Metagenome
  • Metagenomics/methods
  • Sequence Analysis, DNA/methods

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