A comparison of single-coverage and multi-coverage metagenomic binning reveals extensive hidden contamination

J. Mattock; M. Watson

doi:10.1038/s41592-023-01934-8

A comparison of single-coverage and multi-coverage metagenomic binning reveals extensive hidden contamination

J. Mattock, M. Watson

Research output: Contribution to journal › Short communication › peer-review

22 Citations (Scopus)

59 Downloads (Pure)

Abstract

Metagenomic binning has revolutionized the study of uncultured microorganisms. Here we compare single- and multi-coverage binning on the same set of samples, and demonstrate that multi-coverage binning produces better results than single-coverage binning and identifies contaminant contigs and chimeric bins that other approaches miss. While resource expensive, multi-coverage binning is a superior approach and should always be performed over single-coverage binning.

Original language	English
Pages (from-to)	1170-1173
Number of pages	4
Journal	Nature Methods
Volume	20
Issue number	8
Early online date	29 Jun 2023
DOIs	https://doi.org/10.1038/s41592-023-01934-8
Publication status	Print publication - Aug 2023
Externally published	Yes

Bibliographical note

Keywords

Algorithms
Metagenome
Metagenomics/methods
Sequence Analysis, DNA/methods

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10.1038/s41592-023-01934-8

57714_3_art_18_5917_v2Accepted author manuscript, 55.1 KBLicence: CC BY-NC
Supplementary_Information_v3Accepted author manuscript, 181 KBLicence: CC BY-NC
figure2Accepted author manuscript, 187 KBLicence: CC BY-NC
figure1Accepted author manuscript, 188 KBLicence: CC BY-NC

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85163736117&doi=10.1038%2fs41592-023-01934-8&partnerID=40&md5=c99e5b407e005fc910d3f0b012b31970

Cite this

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abstract = "Metagenomic binning has revolutionized the study of uncultured microorganisms. Here we compare single- and multi-coverage binning on the same set of samples, and demonstrate that multi-coverage binning produces better results than single-coverage binning and identifies contaminant contigs and chimeric bins that other approaches miss. While resource expensive, multi-coverage binning is a superior approach and should always be performed over single-coverage binning.",

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AU - Mattock, J.

AU - Watson, M.

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N2 - Metagenomic binning has revolutionized the study of uncultured microorganisms. Here we compare single- and multi-coverage binning on the same set of samples, and demonstrate that multi-coverage binning produces better results than single-coverage binning and identifies contaminant contigs and chimeric bins that other approaches miss. While resource expensive, multi-coverage binning is a superior approach and should always be performed over single-coverage binning.

AB - Metagenomic binning has revolutionized the study of uncultured microorganisms. Here we compare single- and multi-coverage binning on the same set of samples, and demonstrate that multi-coverage binning produces better results than single-coverage binning and identifies contaminant contigs and chimeric bins that other approaches miss. While resource expensive, multi-coverage binning is a superior approach and should always be performed over single-coverage binning.

KW - Algorithms

KW - Metagenome

KW - Metagenomics/methods

KW - Sequence Analysis, DNA/methods

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DO - 10.1038/s41592-023-01934-8

M3 - Short communication

C2 - 37386187

SN - 1548-7091

VL - 20

SP - 1170

EP - 1173

JO - Nature Methods

JF - Nature Methods

IS - 8

ER -

A comparison of single-coverage and multi-coverage metagenomic binning reveals extensive hidden contamination

Abstract

Bibliographical note

Keywords

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