A house dust mite allergen homologue from poultry red mite Dermanyssus gallinae (De Geer).

AJ Nisbet, JF Huntley, A Mackellar, N Sparks, R McDevitt

Research output: Contribution to journalArticleResearchpeer-review

19 Citations (Scopus)

Abstract

Tropomyosin is an allergenic, actin‐binding protein and a proposed vaccine candidate from several species of parasite. Tropomyosin cDNA, obtained by polymerase chain reaction (PCR) amplification from Dermanyssus gallinae RNA, encoded a predicted protein with 89% and 88% identity to tropomyosins from the ticks Boophilus microplus and Haemaphysalis longicornis, respectively, and 85% identity to the house dust mite (HDM) tropomyosin Der p 10. Mouse antibodies raised against HDM tropomyosin reacted with a band of 38 kDa on Western blots of D. gallinae extract, consistent with the molecular masses of acarine tropomyosins and the putative product of the cDNA encoding D. gallinae tropomyosin. When the same preparation of D. gallinae proteins was used in Western blots with serum from infested hens, the IgY component of the serum bound to a number of mite proteins, but not to tropomyosin, indicating that hens are not directly exposed to this allergen during a natural infestation. Immunolocalization of tropomyosin in mites indicated a ubiquitous distribution of the molecule in mite tissues. Immunolocalization and Western blotting also indicated that poultry red mites ingest host IgY.
Original languageEnglish
Pages (from-to)401-405
JournalParasite Immunology
Volume28
Issue number8
DOIs
Publication statusPrint publication - Aug 2006

Fingerprint

Dermatophagoides Antigens
Tropomyosin
Mites
Poultry
Pyroglyphidae
Western Blotting
Proteins
Complementary DNA
Ticks
Serum
Allergens
Parasites
Vaccines
RNA
Polymerase Chain Reaction

Keywords

  • Der p 10
  • Dermanyssus gallinae
  • Mite allergen
  • Poultry red mite
  • Tropomyosin

Cite this

Nisbet, AJ ; Huntley, JF ; Mackellar, A ; Sparks, N ; McDevitt, R. / A house dust mite allergen homologue from poultry red mite Dermanyssus gallinae (De Geer). In: Parasite Immunology. 2006 ; Vol. 28, No. 8. pp. 401-405.
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title = "A house dust mite allergen homologue from poultry red mite Dermanyssus gallinae (De Geer).",
abstract = "Tropomyosin is an allergenic, actin‐binding protein and a proposed vaccine candidate from several species of parasite. Tropomyosin cDNA, obtained by polymerase chain reaction (PCR) amplification from Dermanyssus gallinae RNA, encoded a predicted protein with 89{\%} and 88{\%} identity to tropomyosins from the ticks Boophilus microplus and Haemaphysalis longicornis, respectively, and 85{\%} identity to the house dust mite (HDM) tropomyosin Der p 10. Mouse antibodies raised against HDM tropomyosin reacted with a band of 38 kDa on Western blots of D. gallinae extract, consistent with the molecular masses of acarine tropomyosins and the putative product of the cDNA encoding D. gallinae tropomyosin. When the same preparation of D. gallinae proteins was used in Western blots with serum from infested hens, the IgY component of the serum bound to a number of mite proteins, but not to tropomyosin, indicating that hens are not directly exposed to this allergen during a natural infestation. Immunolocalization of tropomyosin in mites indicated a ubiquitous distribution of the molecule in mite tissues. Immunolocalization and Western blotting also indicated that poultry red mites ingest host IgY.",
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A house dust mite allergen homologue from poultry red mite Dermanyssus gallinae (De Geer). / Nisbet, AJ; Huntley, JF; Mackellar, A; Sparks, N; McDevitt, R.

In: Parasite Immunology, Vol. 28, No. 8, 08.2006, p. 401-405.

Research output: Contribution to journalArticleResearchpeer-review

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