A meta-analysis of fumarate effects on methane production in ruminal batch cultures

E M Ungerfeld, R A Kohn, R J Wallace, C J Newbold

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The objective of this study was to understand the effects of fumarate addition on methane (CH4) and VFA production in the rumen through a meta-analysis of its effects on ruminal batch cultures. Because the reduction of fumarate to succinate can draw electrons away from ruminal methanogenesis, fumarate has been studied as a potential feed additive to decrease CH4 production in ruminants. The average decrease in CH4 in batch cultures was of 0.037 micromol/micromol of added fumarate, which is considerably lower than 0.25 micromol/micromol, the decrease predicted from the stoichiometry of the reactions involved. One reason that fumarate was not effective at decreasing CH4 in batch cultures was that only an average of 48% of added fumarate appeared to be converted to propionate. Secondly, the incorporation of reducing equivalents in the conversion of fumarate to propionate was almost entirely offset by their release from an average of 20% of added fumarate that appeared to be converted to acetate. Thermodynamic calculations indicated that the conversion of added fumarate to both propionate and acetate was feasible. Fumarate appears to be more effective in decreasing CH4 production and increasing propionate in continuous culture than in batch culture. This suggests that microbial adaptation to fumarate metabolism can be important. Variation in populations of fumarate-reducers, methanogens, and protozoa could all be involved. Fumarate supplementation for an extended period may result in the amplification of otherwise small populations of fumarate-reducers. Addition of some of these organisms may be helpful to improve fumarate conversion to propionate. Strategies based on enhancing the rumen's capacity to convert fumarate to propionate by maintaining a low fumarate concentration have been effective. Thermodynamic considerations should be taken into account when designing strategies for CH4 abatement through the addition of external electron acceptors.

Original languageEnglish
Pages (from-to)2556-63
Number of pages8
JournalJournal of Animal Science
Volume85
Issue number10
DOIs
Publication statusPrint publication - Oct 2007
Externally publishedYes

Fingerprint

Fumarates
Batch Cell Culture Techniques
Methane
methane production
meta-analysis
Meta-Analysis
Propionates
propionates
methane
fumarates
Rumen
Thermodynamics
thermodynamics
rumen
Acetates
acetates
electrons
Electrons
methanogens
feed additives

Keywords

  • Adaptation, Physiological
  • Animal Feed
  • Animal Nutritional Physiological Phenomena
  • Animals
  • Bacteria, Anaerobic/metabolism
  • Fatty Acids, Volatile/biosynthesis
  • Fermentation
  • Fumarates/metabolism
  • Methane/biosynthesis
  • Propionates/metabolism
  • Rumen/metabolism
  • Sheep/metabolism

Cite this

Ungerfeld, E M ; Kohn, R A ; Wallace, R J ; Newbold, C J. / A meta-analysis of fumarate effects on methane production in ruminal batch cultures. In: Journal of Animal Science. 2007 ; Vol. 85, No. 10. pp. 2556-63.
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A meta-analysis of fumarate effects on methane production in ruminal batch cultures. / Ungerfeld, E M; Kohn, R A; Wallace, R J; Newbold, C J.

In: Journal of Animal Science, Vol. 85, No. 10, 10.2007, p. 2556-63.

Research output: Contribution to journalArticle

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T1 - A meta-analysis of fumarate effects on methane production in ruminal batch cultures

AU - Ungerfeld, E M

AU - Kohn, R A

AU - Wallace, R J

AU - Newbold, C J

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AB - The objective of this study was to understand the effects of fumarate addition on methane (CH4) and VFA production in the rumen through a meta-analysis of its effects on ruminal batch cultures. Because the reduction of fumarate to succinate can draw electrons away from ruminal methanogenesis, fumarate has been studied as a potential feed additive to decrease CH4 production in ruminants. The average decrease in CH4 in batch cultures was of 0.037 micromol/micromol of added fumarate, which is considerably lower than 0.25 micromol/micromol, the decrease predicted from the stoichiometry of the reactions involved. One reason that fumarate was not effective at decreasing CH4 in batch cultures was that only an average of 48% of added fumarate appeared to be converted to propionate. Secondly, the incorporation of reducing equivalents in the conversion of fumarate to propionate was almost entirely offset by their release from an average of 20% of added fumarate that appeared to be converted to acetate. Thermodynamic calculations indicated that the conversion of added fumarate to both propionate and acetate was feasible. Fumarate appears to be more effective in decreasing CH4 production and increasing propionate in continuous culture than in batch culture. This suggests that microbial adaptation to fumarate metabolism can be important. Variation in populations of fumarate-reducers, methanogens, and protozoa could all be involved. Fumarate supplementation for an extended period may result in the amplification of otherwise small populations of fumarate-reducers. Addition of some of these organisms may be helpful to improve fumarate conversion to propionate. Strategies based on enhancing the rumen's capacity to convert fumarate to propionate by maintaining a low fumarate concentration have been effective. Thermodynamic considerations should be taken into account when designing strategies for CH4 abatement through the addition of external electron acceptors.

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KW - Animal Nutritional Physiological Phenomena

KW - Animals

KW - Bacteria, Anaerobic/metabolism

KW - Fatty Acids, Volatile/biosynthesis

KW - Fermentation

KW - Fumarates/metabolism

KW - Methane/biosynthesis

KW - Propionates/metabolism

KW - Rumen/metabolism

KW - Sheep/metabolism

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DO - 10.2527/jas.2006-674

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