A novel astrovirus associated with encephalitis and ganglionitis in domestic sheep

F Pfaff, K Schlottau, S Scholes, A Courtenay, B Hoffmann, D Hoper, M Beer*

*Corresponding author for this work

Research output: Contribution to journalArticle

29 Citations (Scopus)
7 Downloads (Pure)

Abstract

In June 2013, a 4-year-old Welsh Mountain ewe and in March 2014 a 10-day-old lamb of the same breed and the same flock presented progressive neurological signs including depressed sensorium, tremor, and unusual behaviour. Neuropathological examination of the brain and spinal cord detected non-suppurative polioencephalomyelitis and dorsal root ganglionitis, characteristic of a neurotropic viral agent in both sheep. Metagenomic analysis of different tissue samples from both animals identified a novel Ovine Astrovirus (OvAstV). The presence of viral genome in the central nervous system was confirmed by RT-qPCR. Although the cases presented nine months apart, the identified OvAstV shared nearly identical sequences, differing in only three nucleotide positions across the complete genome. Phylogenetic analysis revealed a close relation of OvAstV to neurotropic bovine astroviruses and an enteric OvAstV. In conclusion, these are the first reported cases of astrovirus infection in domestic sheep that were associated with encephalitis and ganglionitis.
Original languageEnglish
Pages (from-to)677 - 682
Number of pages6
JournalTransboundary and Emerging Diseases
Volume64
Issue number3
Early online date22 Feb 2017
DOIs
Publication statusFirst published - 22 Feb 2017

Fingerprint

Domestic Sheep
Encephalitis
Sheep
Metagenomics
Spinal Nerve Roots
Viral Genome
Tremor
Spinal Cord
Nucleotides
Central Nervous System
Genome
Brain
Infection

Bibliographical note

© 2017 Blackwell Verlag GmbH

Keywords

  • Encephalitis
  • High-throughput nucleotide sequencing
  • Mamastrovirus
  • Metagenomics
  • Sheep
  • Viral

Cite this

Pfaff, F., Schlottau, K., Scholes, S., Courtenay, A., Hoffmann, B., Hoper, D., & Beer, M. (2017). A novel astrovirus associated with encephalitis and ganglionitis in domestic sheep. Transboundary and Emerging Diseases, 64(3), 677 - 682. https://doi.org/10.1111/tbed.12623
Pfaff, F ; Schlottau, K ; Scholes, S ; Courtenay, A ; Hoffmann, B ; Hoper, D ; Beer, M. / A novel astrovirus associated with encephalitis and ganglionitis in domestic sheep. In: Transboundary and Emerging Diseases. 2017 ; Vol. 64, No. 3. pp. 677 - 682.
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Pfaff, F, Schlottau, K, Scholes, S, Courtenay, A, Hoffmann, B, Hoper, D & Beer, M 2017, 'A novel astrovirus associated with encephalitis and ganglionitis in domestic sheep', Transboundary and Emerging Diseases, vol. 64, no. 3, pp. 677 - 682. https://doi.org/10.1111/tbed.12623

A novel astrovirus associated with encephalitis and ganglionitis in domestic sheep. / Pfaff, F; Schlottau, K; Scholes, S; Courtenay, A; Hoffmann, B; Hoper, D; Beer, M.

In: Transboundary and Emerging Diseases, Vol. 64, No. 3, 22.02.2017, p. 677 - 682.

Research output: Contribution to journalArticle

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AU - Pfaff, F

AU - Schlottau, K

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AU - Courtenay, A

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AU - Beer, M

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AB - In June 2013, a 4-year-old Welsh Mountain ewe and in March 2014 a 10-day-old lamb of the same breed and the same flock presented progressive neurological signs including depressed sensorium, tremor, and unusual behaviour. Neuropathological examination of the brain and spinal cord detected non-suppurative polioencephalomyelitis and dorsal root ganglionitis, characteristic of a neurotropic viral agent in both sheep. Metagenomic analysis of different tissue samples from both animals identified a novel Ovine Astrovirus (OvAstV). The presence of viral genome in the central nervous system was confirmed by RT-qPCR. Although the cases presented nine months apart, the identified OvAstV shared nearly identical sequences, differing in only three nucleotide positions across the complete genome. Phylogenetic analysis revealed a close relation of OvAstV to neurotropic bovine astroviruses and an enteric OvAstV. In conclusion, these are the first reported cases of astrovirus infection in domestic sheep that were associated with encephalitis and ganglionitis.

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Pfaff F, Schlottau K, Scholes S, Courtenay A, Hoffmann B, Hoper D et al. A novel astrovirus associated with encephalitis and ganglionitis in domestic sheep. Transboundary and Emerging Diseases. 2017 Feb 22;64(3):677 - 682. https://doi.org/10.1111/tbed.12623