A novel bacterial blight resistance gene from Oryza nivara mapped to 38 kb region on chromosome 4L and transferred to Oryza sativa L

Kuljit K. Cheema, Navjit K. Grewal, Yogesh Vikal, Rajiv Sharma, Jagjeet S. Lore, Aparna Das, Dharminder Bhatia, Ritu Mahajan, Vikas Gupta, Tajinder S. Bharaj, Kuldeep Singh

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Abstract

Bacterial blight (BB) of rice caused by Xanthomonas oryzae pv oryzae ( Xoo ) is one of the major constraints to productivity in South-East Asia. The strategy of using major genes, singly or in combination, continues to be the most effective approach for BB management. Currently, more than two dozen genes have been designated but not all the known genes are effective against all the prevalent pathotypes. The challenge, therefore, is to continue to expand the gene pool of effective and potentially durable resistance genes. Wild species constitute an important reservoir of the resistance genes including BB. An accession of Oryza nivara (IRGC 81825) was found to be resistant to all the seven Xoo pathotypes prevalent in northern states of India. Inheritance and mapping of resistance in O. nivara was studied by using F 2 , BC 2 F 2 , BC 3 F 1 and BC 3 F 2 progenies of the cross involving Oryza sativa cv PR114 and the O. nivara acc. 81825 using the most virulent Xoo pathotype. Genetic analysis of the segregating progenies revealed that the BB resistance in O. nivara was conditioned by a single dominant gene. Bulked segregant analysis (BSA) of F 2 population using 191 polymorphic SSR markers identified a ∼35 centiMorgans (cM) chromosomal region on 4L, bracketed by RM317 and RM562, to be associated with BB resistance. Screening of BC 3 F 1 and BC 2 F 2 progenies and their genotyping with more than 30 polymorphic SSR markers in the region, covering Bacterial artificial chromosome (BAC) clone OSJNBb0085C12, led to mapping of the resistance gene between the STS markers based on annotated genes LOC_Os04g53060 and LOC_Os04g53120, which is ∼38·4 kb. Since none of the known Xa genes, which are mapped on chromosome 4L, are effective against the Xoo pathotypes tested, the BB resistance gene identified and transferred from O. nivara is novel and is tentatively designated as Xa30 ( t ). Homozygous resistant BC 3 F 3 progenies with smallest introgression region have been identified.
Original languageEnglish
Pages (from-to)397-407
Number of pages11
JournalGenetics Research
Volume90
Issue number5
DOIs
Publication statusPrint publication - 2008

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Chromosomes
Xanthomonas
Genes
Oryza
Bacterial Artificial Chromosomes
Gene Pool
Bacterial Genes
Dominant Genes
Far East
Chromosome Mapping
India
Clone Cells
Population

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Cheema, Kuljit K. ; Grewal, Navjit K. ; Vikal, Yogesh ; Sharma, Rajiv ; Lore, Jagjeet S. ; Das, Aparna ; Bhatia, Dharminder ; Mahajan, Ritu ; Gupta, Vikas ; Bharaj, Tajinder S. ; Singh, Kuldeep. / A novel bacterial blight resistance gene from Oryza nivara mapped to 38 kb region on chromosome 4L and transferred to Oryza sativa L. In: Genetics Research. 2008 ; Vol. 90, No. 5. pp. 397-407.
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title = "A novel bacterial blight resistance gene from Oryza nivara mapped to 38 kb region on chromosome 4L and transferred to Oryza sativa L",
abstract = "Bacterial blight (BB) of rice caused by Xanthomonas oryzae pv oryzae ( Xoo ) is one of the major constraints to productivity in South-East Asia. The strategy of using major genes, singly or in combination, continues to be the most effective approach for BB management. Currently, more than two dozen genes have been designated but not all the known genes are effective against all the prevalent pathotypes. The challenge, therefore, is to continue to expand the gene pool of effective and potentially durable resistance genes. Wild species constitute an important reservoir of the resistance genes including BB. An accession of Oryza nivara (IRGC 81825) was found to be resistant to all the seven Xoo pathotypes prevalent in northern states of India. Inheritance and mapping of resistance in O. nivara was studied by using F 2 , BC 2 F 2 , BC 3 F 1 and BC 3 F 2 progenies of the cross involving Oryza sativa cv PR114 and the O. nivara acc. 81825 using the most virulent Xoo pathotype. Genetic analysis of the segregating progenies revealed that the BB resistance in O. nivara was conditioned by a single dominant gene. Bulked segregant analysis (BSA) of F 2 population using 191 polymorphic SSR markers identified a ∼35 centiMorgans (cM) chromosomal region on 4L, bracketed by RM317 and RM562, to be associated with BB resistance. Screening of BC 3 F 1 and BC 2 F 2 progenies and their genotyping with more than 30 polymorphic SSR markers in the region, covering Bacterial artificial chromosome (BAC) clone OSJNBb0085C12, led to mapping of the resistance gene between the STS markers based on annotated genes LOC_Os04g53060 and LOC_Os04g53120, which is ∼38·4 kb. Since none of the known Xa genes, which are mapped on chromosome 4L, are effective against the Xoo pathotypes tested, the BB resistance gene identified and transferred from O. nivara is novel and is tentatively designated as Xa30 ( t ). Homozygous resistant BC 3 F 3 progenies with smallest introgression region have been identified.",
author = "Cheema, {Kuljit K.} and Grewal, {Navjit K.} and Yogesh Vikal and Rajiv Sharma and Lore, {Jagjeet S.} and Aparna Das and Dharminder Bhatia and Ritu Mahajan and Vikas Gupta and Bharaj, {Tajinder S.} and Kuldeep Singh",
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Cheema, KK, Grewal, NK, Vikal, Y, Sharma, R, Lore, JS, Das, A, Bhatia, D, Mahajan, R, Gupta, V, Bharaj, TS & Singh, K 2008, 'A novel bacterial blight resistance gene from Oryza nivara mapped to 38 kb region on chromosome 4L and transferred to Oryza sativa L', Genetics Research, vol. 90, no. 5, pp. 397-407. https://doi.org/10.1017/S0016672308009786

A novel bacterial blight resistance gene from Oryza nivara mapped to 38 kb region on chromosome 4L and transferred to Oryza sativa L. / Cheema, Kuljit K.; Grewal, Navjit K.; Vikal, Yogesh; Sharma, Rajiv; Lore, Jagjeet S.; Das, Aparna; Bhatia, Dharminder; Mahajan, Ritu; Gupta, Vikas; Bharaj, Tajinder S.; Singh, Kuldeep.

In: Genetics Research, Vol. 90, No. 5, 2008, p. 397-407.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - A novel bacterial blight resistance gene from Oryza nivara mapped to 38 kb region on chromosome 4L and transferred to Oryza sativa L

AU - Cheema, Kuljit K.

AU - Grewal, Navjit K.

AU - Vikal, Yogesh

AU - Sharma, Rajiv

AU - Lore, Jagjeet S.

AU - Das, Aparna

AU - Bhatia, Dharminder

AU - Mahajan, Ritu

AU - Gupta, Vikas

AU - Bharaj, Tajinder S.

AU - Singh, Kuldeep

PY - 2008

Y1 - 2008

N2 - Bacterial blight (BB) of rice caused by Xanthomonas oryzae pv oryzae ( Xoo ) is one of the major constraints to productivity in South-East Asia. The strategy of using major genes, singly or in combination, continues to be the most effective approach for BB management. Currently, more than two dozen genes have been designated but not all the known genes are effective against all the prevalent pathotypes. The challenge, therefore, is to continue to expand the gene pool of effective and potentially durable resistance genes. Wild species constitute an important reservoir of the resistance genes including BB. An accession of Oryza nivara (IRGC 81825) was found to be resistant to all the seven Xoo pathotypes prevalent in northern states of India. Inheritance and mapping of resistance in O. nivara was studied by using F 2 , BC 2 F 2 , BC 3 F 1 and BC 3 F 2 progenies of the cross involving Oryza sativa cv PR114 and the O. nivara acc. 81825 using the most virulent Xoo pathotype. Genetic analysis of the segregating progenies revealed that the BB resistance in O. nivara was conditioned by a single dominant gene. Bulked segregant analysis (BSA) of F 2 population using 191 polymorphic SSR markers identified a ∼35 centiMorgans (cM) chromosomal region on 4L, bracketed by RM317 and RM562, to be associated with BB resistance. Screening of BC 3 F 1 and BC 2 F 2 progenies and their genotyping with more than 30 polymorphic SSR markers in the region, covering Bacterial artificial chromosome (BAC) clone OSJNBb0085C12, led to mapping of the resistance gene between the STS markers based on annotated genes LOC_Os04g53060 and LOC_Os04g53120, which is ∼38·4 kb. Since none of the known Xa genes, which are mapped on chromosome 4L, are effective against the Xoo pathotypes tested, the BB resistance gene identified and transferred from O. nivara is novel and is tentatively designated as Xa30 ( t ). Homozygous resistant BC 3 F 3 progenies with smallest introgression region have been identified.

AB - Bacterial blight (BB) of rice caused by Xanthomonas oryzae pv oryzae ( Xoo ) is one of the major constraints to productivity in South-East Asia. The strategy of using major genes, singly or in combination, continues to be the most effective approach for BB management. Currently, more than two dozen genes have been designated but not all the known genes are effective against all the prevalent pathotypes. The challenge, therefore, is to continue to expand the gene pool of effective and potentially durable resistance genes. Wild species constitute an important reservoir of the resistance genes including BB. An accession of Oryza nivara (IRGC 81825) was found to be resistant to all the seven Xoo pathotypes prevalent in northern states of India. Inheritance and mapping of resistance in O. nivara was studied by using F 2 , BC 2 F 2 , BC 3 F 1 and BC 3 F 2 progenies of the cross involving Oryza sativa cv PR114 and the O. nivara acc. 81825 using the most virulent Xoo pathotype. Genetic analysis of the segregating progenies revealed that the BB resistance in O. nivara was conditioned by a single dominant gene. Bulked segregant analysis (BSA) of F 2 population using 191 polymorphic SSR markers identified a ∼35 centiMorgans (cM) chromosomal region on 4L, bracketed by RM317 and RM562, to be associated with BB resistance. Screening of BC 3 F 1 and BC 2 F 2 progenies and their genotyping with more than 30 polymorphic SSR markers in the region, covering Bacterial artificial chromosome (BAC) clone OSJNBb0085C12, led to mapping of the resistance gene between the STS markers based on annotated genes LOC_Os04g53060 and LOC_Os04g53120, which is ∼38·4 kb. Since none of the known Xa genes, which are mapped on chromosome 4L, are effective against the Xoo pathotypes tested, the BB resistance gene identified and transferred from O. nivara is novel and is tentatively designated as Xa30 ( t ). Homozygous resistant BC 3 F 3 progenies with smallest introgression region have been identified.

UR - http://www.mendeley.com/research/novel-bacterial-blight-resistance-gene-oryza-nivara-mapped-38-kb-region-chromosome-4l-transferred-or

U2 - 10.1017/S0016672308009786

DO - 10.1017/S0016672308009786

M3 - Article

VL - 90

SP - 397

EP - 407

JO - Genetical Research

JF - Genetical Research

SN - 0016-6723

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ER -