A Novel Whole Yeast-Based Subunit Oral Vaccine Against Eimeria tenella in Chickens

Francesca Soutter, Dirk Werling*, Matthew Nolan, Tatiana Küster, Elizabeth Attree, Virginia Marugán-Hernández, Sungwon Kim, Fiona M Tomley, Damer P Blake

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)
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Cheap, easy-to-produce oral vaccines are needed for control of coccidiosis in chickens to reduce the impact of this disease on welfare and economic performance. Saccharomyces cerevisiae yeast expressing three Eimeria tenella antigens were developed and delivered as heat-killed, freeze-dried whole yeast oral vaccines to chickens in four separate studies. After vaccination, E. tenella replication was reduced following low dose challenge (250 oocysts) in Hy-Line Brown layer chickens (p<0.01). Similarly, caecal lesion score was reduced in Hy-Line Brown layer chickens vaccinated using a mixture of S. cerevisiae expressing EtAMA1, EtIMP1 and EtMIC3 following pathogenic-level challenge (4,000 E. tenella oocysts; p<0.01). Mean body weight gain post-challenge with 15,000 E. tenella oocysts was significantly increased in vaccinated Cobb500 broiler chickens compared to mock-vaccinated controls (p<0.01). Thus, inactivated recombinant yeast vaccines offer cost-effective and scalable opportunities for control of coccidiosis, with relevance to broiler production and chickens reared in low-and middle-income countries (LMICs).

Original languageEnglish
Article number809711
JournalFrontiers in Immunology
Early online date2 Feb 2022
Publication statusFirst published - 2 Feb 2022

Bibliographical note

Copyright © 2022 Soutter, Werling, Nolan, Küster, Attree, Marugán-Hernández, Kim, Tomley and Blake.


  • chicken
  • Eimeria tenella
  • Saccharomyces cerevisiae
  • vaccine
  • yeast


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