Abstract
Promiscuous activity of a glycosyltransferase was exploited to polymerise glucose from UDP-glucoseviathe generation of β-1,4-glycosidic linkages. The biocatalyst was incorporated into biocatalytic cascades and chemo-enzymatic strategies to synthesise cello-oligosaccharides with tailored functionalities on a scale suitable for employment in mass spectrometry-based assays. The resulting glycan structures enabled reporting of the activity and selectivity of celluloltic enzymes.
Original language | English |
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Pages (from-to) | 5529-5533 |
Number of pages | 5 |
Journal | Organic and Biomolecular Chemistry |
Volume | 19 |
Issue number | 25 |
Early online date | 1 Jun 2021 |
DOIs | |
Publication status | First published - 1 Jun 2021 |