A re-appraisal of the diversity of the methanogens associated with the rumen ciliates

M Regensbogenova, N R McEwan, P Javorsky, S Kisidayova, T Michalowski, C J Newbold, J H P Hackstein, P Pristas

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The diversity of methanogenic archaea associated with different species of ciliated protozoa in the rumen was analysed. Partial fragments of archaeal SSU rRNA genes were amplified from DNA isolated from single cells from the rumen protozoal species Metadinium medium, Entodinium furca, Ophryoscolex caudatus and Diplodinium dentatum. Sequence analysis of these fragments indicated that although all of the new isolates clustered with sequences previously described for methanogens, there was a difference in the relative distribution of sequences detected here as compared to that of previous work. In addition, many of the novel sequences, although clearly of archaeal origin have relatively low identity to the sequences in database which are most closely related to them.

Original languageEnglish
Pages (from-to)307-13
Number of pages7
JournalFEMS Microbiology Letters
Volume238
Issue number2
DOIs
Publication statusPrint publication - 15 Sep 2004
Externally publishedYes

Fingerprint

Rumen
Archaea
rRNA Genes
Sequence Analysis
Databases
DNA

Keywords

  • Animals
  • Ciliophora/microbiology
  • DNA, Archaeal/analysis
  • DNA, Ribosomal/analysis
  • Euryarchaeota/classification
  • Phylogeny
  • RNA, Archaeal/analysis
  • RNA, Ribosomal, 16S/analysis
  • Rumen/microbiology
  • Sheep

Cite this

Regensbogenova, M., McEwan, N. R., Javorsky, P., Kisidayova, S., Michalowski, T., Newbold, C. J., ... Pristas, P. (2004). A re-appraisal of the diversity of the methanogens associated with the rumen ciliates. FEMS Microbiology Letters, 238(2), 307-13. https://doi.org/10.1016/j.femsle.2004.07.049
Regensbogenova, M ; McEwan, N R ; Javorsky, P ; Kisidayova, S ; Michalowski, T ; Newbold, C J ; Hackstein, J H P ; Pristas, P. / A re-appraisal of the diversity of the methanogens associated with the rumen ciliates. In: FEMS Microbiology Letters. 2004 ; Vol. 238, No. 2. pp. 307-13.
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Regensbogenova, M, McEwan, NR, Javorsky, P, Kisidayova, S, Michalowski, T, Newbold, CJ, Hackstein, JHP & Pristas, P 2004, 'A re-appraisal of the diversity of the methanogens associated with the rumen ciliates', FEMS Microbiology Letters, vol. 238, no. 2, pp. 307-13. https://doi.org/10.1016/j.femsle.2004.07.049

A re-appraisal of the diversity of the methanogens associated with the rumen ciliates. / Regensbogenova, M; McEwan, N R; Javorsky, P; Kisidayova, S; Michalowski, T; Newbold, C J; Hackstein, J H P; Pristas, P.

In: FEMS Microbiology Letters, Vol. 238, No. 2, 15.09.2004, p. 307-13.

Research output: Contribution to journalArticle

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AU - Regensbogenova, M

AU - McEwan, N R

AU - Javorsky, P

AU - Kisidayova, S

AU - Michalowski, T

AU - Newbold, C J

AU - Hackstein, J H P

AU - Pristas, P

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AB - The diversity of methanogenic archaea associated with different species of ciliated protozoa in the rumen was analysed. Partial fragments of archaeal SSU rRNA genes were amplified from DNA isolated from single cells from the rumen protozoal species Metadinium medium, Entodinium furca, Ophryoscolex caudatus and Diplodinium dentatum. Sequence analysis of these fragments indicated that although all of the new isolates clustered with sequences previously described for methanogens, there was a difference in the relative distribution of sequences detected here as compared to that of previous work. In addition, many of the novel sequences, although clearly of archaeal origin have relatively low identity to the sequences in database which are most closely related to them.

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KW - DNA, Ribosomal/analysis

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KW - RNA, Ribosomal, 16S/analysis

KW - Rumen/microbiology

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