A re-appraisal of the diversity of the methanogens associated with the rumen ciliates

M Regensbogenova; N R McEwan; P Javorsky; S Kisidayova; T Michalowski; C J Newbold; J H P Hackstein; P Pristas

doi:10.1016/j.femsle.2004.07.049

A re-appraisal of the diversity of the methanogens associated with the rumen ciliates

M Regensbogenova, N R McEwan, P Javorsky, S Kisidayova, T Michalowski, C J Newbold, J H P Hackstein, P Pristas

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

The diversity of methanogenic archaea associated with different species of ciliated protozoa in the rumen was analysed. Partial fragments of archaeal SSU rRNA genes were amplified from DNA isolated from single cells from the rumen protozoal species Metadinium medium, Entodinium furca, Ophryoscolex caudatus and Diplodinium dentatum. Sequence analysis of these fragments indicated that although all of the new isolates clustered with sequences previously described for methanogens, there was a difference in the relative distribution of sequences detected here as compared to that of previous work. In addition, many of the novel sequences, although clearly of archaeal origin have relatively low identity to the sequences in database which are most closely related to them.

Original language	English
Pages (from-to)	307-13
Number of pages	7
Journal	FEMS Microbiology Letters
Volume	238
Issue number	2
DOIs	https://doi.org/10.1016/j.femsle.2004.07.049
Publication status	Print publication - 15 Sept 2004
Externally published	Yes

Keywords

Animals
Ciliophora/microbiology
DNA, Archaeal/analysis
DNA, Ribosomal/analysis
Euryarchaeota/classification
Phylogeny
RNA, Archaeal/analysis
RNA, Ribosomal, 16S/analysis
Rumen/microbiology
Sheep

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10.1016/j.femsle.2004.07.049

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title = "A re-appraisal of the diversity of the methanogens associated with the rumen ciliates",

abstract = "The diversity of methanogenic archaea associated with different species of ciliated protozoa in the rumen was analysed. Partial fragments of archaeal SSU rRNA genes were amplified from DNA isolated from single cells from the rumen protozoal species Metadinium medium, Entodinium furca, Ophryoscolex caudatus and Diplodinium dentatum. Sequence analysis of these fragments indicated that although all of the new isolates clustered with sequences previously described for methanogens, there was a difference in the relative distribution of sequences detected here as compared to that of previous work. In addition, many of the novel sequences, although clearly of archaeal origin have relatively low identity to the sequences in database which are most closely related to them.",

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author = "M Regensbogenova and McEwan, {N R} and P Javorsky and S Kisidayova and T Michalowski and Newbold, {C J} and Hackstein, {J H P} and P Pristas",

year = "2004",

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doi = "10.1016/j.femsle.2004.07.049",

language = "English",

volume = "238",

pages = "307--13",

journal = "FEMS Microbiology Letters",

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AU - Regensbogenova, M

AU - McEwan, N R

AU - Javorsky, P

AU - Kisidayova, S

AU - Michalowski, T

AU - Newbold, C J

AU - Hackstein, J H P

AU - Pristas, P

PY - 2004/9/15

Y1 - 2004/9/15

N2 - The diversity of methanogenic archaea associated with different species of ciliated protozoa in the rumen was analysed. Partial fragments of archaeal SSU rRNA genes were amplified from DNA isolated from single cells from the rumen protozoal species Metadinium medium, Entodinium furca, Ophryoscolex caudatus and Diplodinium dentatum. Sequence analysis of these fragments indicated that although all of the new isolates clustered with sequences previously described for methanogens, there was a difference in the relative distribution of sequences detected here as compared to that of previous work. In addition, many of the novel sequences, although clearly of archaeal origin have relatively low identity to the sequences in database which are most closely related to them.

AB - The diversity of methanogenic archaea associated with different species of ciliated protozoa in the rumen was analysed. Partial fragments of archaeal SSU rRNA genes were amplified from DNA isolated from single cells from the rumen protozoal species Metadinium medium, Entodinium furca, Ophryoscolex caudatus and Diplodinium dentatum. Sequence analysis of these fragments indicated that although all of the new isolates clustered with sequences previously described for methanogens, there was a difference in the relative distribution of sequences detected here as compared to that of previous work. In addition, many of the novel sequences, although clearly of archaeal origin have relatively low identity to the sequences in database which are most closely related to them.

KW - Animals

KW - Ciliophora/microbiology

KW - DNA, Archaeal/analysis

KW - DNA, Ribosomal/analysis

KW - Euryarchaeota/classification

KW - Phylogeny

KW - RNA, Archaeal/analysis

KW - RNA, Ribosomal, 16S/analysis

KW - Rumen/microbiology

KW - Sheep

U2 - 10.1016/j.femsle.2004.07.049

DO - 10.1016/j.femsle.2004.07.049

M3 - Article

C2 - 15358415

SN - 0378-1097

VL - 238

SP - 307

EP - 313

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2

ER -

A re-appraisal of the diversity of the methanogens associated with the rumen ciliates

Abstract

Keywords

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