Abstract
We report for the first time the cloning and characterisation of a protozoal enzyme involved in plant cell wall polysaccharide degradation. A cDNA library was constructed from the ruminal protozoan Polyplastron multivesiculatum and a stable clone expressing xylanase activity was isolated. The encoded enzyme belongs to the glycoside hydrolase family 11, and phylogenetic analysis indicates a closer relationship with catalytic domains from Gram-positive bacteria than the other fibrolytic eukaryotes from the rumen, the anaerobic fungi.
Original language | English |
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Pages (from-to) | 145-52 |
Number of pages | 8 |
Journal | FEMS Microbiology Letters |
Volume | 181 |
Issue number | 1 |
DOIs | |
Publication status | Print publication - 1 Dec 1999 |
Externally published | Yes |
Keywords
- Amino Acid Sequence
- Anaerobiosis
- Animals
- Base Sequence
- Ciliophora/enzymology
- DNA, Complementary/genetics
- Electrophoresis, Polyacrylamide Gel
- Glucans/metabolism
- Gram-Positive Bacteria/enzymology
- Immunoblotting
- Molecular Sequence Data
- Phylogeny
- Rumen/parasitology
- Sequence Analysis, DNA
- Xylan Endo-1,3-beta-Xylosidase
- Xylans/metabolism
- Xylosidases/chemistry