Abstract
We examined the activity of phages to control the growth of chicken and swine Salmonella strains in avian (CHIC-8E11), porcine (IPEC-1), and human (HT-29) cell cultures. We optimized a six-phage cocktail by selecting the five most effective myoviruses and a siphovirus that have optimal lysis on prevalent serovars. We observed ∼20% of 7 log10 PFU/well phage and 3-6 log10 CFU bacterial adhesions, and 3-5 log10 CFU bacterial invasion per 2 cm2 of the cultured cells at 2 h post-treatment. The invasive bacteria when plated had a variable reduced susceptibility to the phages. After phage application at an MOI of 10, the prophylaxis regimen had better efficacy at controlling bacterial growth with an up to 6 log10 CFU/well reduction as compared with the 1-2 log10 CFU/well bacterial reduction observed in the remedial and coinfection regimens. Our data support the development of these phages to control salmonellosis in chickens, pigs, and humans.
Original language | English |
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Pages (from-to) | 11-25 |
Number of pages | 15 |
Journal | PHAGE: Therapy, Applications, and Research |
Volume | 4 |
Issue number | 1 |
Early online date | 17 Mar 2023 |
DOIs | |
Publication status | First published - 17 Mar 2023 |
Keywords
- Microbiology (medical)
- Virology
- Microbiology
- Applied Microbiology and Biotechnology
- Molecular Biology
- IPEC-1
- Salmonella enterica
- gastroenteritis
- HT-29
- CHIC-8E11
- bacteriophage therapy