Activity of a bacteriophage cocktail to control Salmonella growth ex vivo in avian, porcine and human epithelial cell cultures

Janet Y. Nale, Buthainah Ahmed, Richard Haigh, Jinyu Shan, Preeda Phothaworn, Parameth Thiennimitr, Angela Garcia, Manal AbuOun, Muna F. Anjum, Sunee Korbsrisate, Edouard E. Galyov, Danish J. Malik, Martha R.J. Clokie

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)
62 Downloads (Pure)

Abstract

We examined the activity of phages to control the growth of chicken and swine Salmonella strains in avian (CHIC-8E11), porcine (IPEC-1), and human (HT-29) cell cultures. We optimized a six-phage cocktail by selecting the five most effective myoviruses and a siphovirus that have optimal lysis on prevalent serovars. We observed ∼20% of 7 log10 PFU/well phage and 3-6 log10 CFU bacterial adhesions, and 3-5 log10 CFU bacterial invasion per 2 cm2 of the cultured cells at 2 h post-treatment. The invasive bacteria when plated had a variable reduced susceptibility to the phages. After phage application at an MOI of 10, the prophylaxis regimen had better efficacy at controlling bacterial growth with an up to 6 log10 CFU/well reduction as compared with the 1-2 log10 CFU/well bacterial reduction observed in the remedial and coinfection regimens. Our data support the development of these phages to control salmonellosis in chickens, pigs, and humans.

Original languageEnglish
Pages (from-to)11-25
Number of pages15
JournalPHAGE: Therapy, Applications, and Research
Volume4
Issue number1
Early online date17 Mar 2023
DOIs
Publication statusFirst published - 17 Mar 2023

Keywords

  • Microbiology (medical)
  • Virology
  • Microbiology
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • IPEC-1
  • Salmonella enterica
  • gastroenteritis
  • HT-29
  • CHIC-8E11
  • bacteriophage therapy

Fingerprint

Dive into the research topics of 'Activity of a bacteriophage cocktail to control Salmonella growth ex vivo in avian, porcine and human epithelial cell cultures'. Together they form a unique fingerprint.

Cite this