An eight-parent multiparent advanced generation inter-cross population for winter-sown wheat: creation, properties, and validation

Ian J Mackay, Pauline Bansept-Basler, Toby Barber, Alison R Bentley, James Cockram, Nick Gosman, Andy J Greenland, Richard Horsnell, Rhian Howells, Donal M O'Sullivan, Gemma A Rose, Phil J Howell

Research output: Contribution to journalArticlepeer-review

103 Citations (Scopus)

Abstract

MAGIC populations represent one of a new generation of crop genetic mapping resources combining high genetic recombination and diversity. We describe the creation and validation of an eight-parent MAGIC population consisting of 1091 F7 lines of winter-sown wheat (Triticum aestivum L.). Analyses based on genotypes from a 90,000-single nucleotide polymorphism (SNP) array find the population to be well-suited as a platform for fine-mapping quantitative trait loci (QTL) and gene isolation. Patterns of linkage disequilibrium (LD) show the population to be highly recombined; genetic marker diversity among the founders was 74% of that captured in a larger set of 64 wheat varieties, and 54% of SNPs segregating among the 64 lines also segregated among the eight founder lines. In contrast, a commonly used reference bi-parental population had only 54% of the diversity of the 64 varieties with 27% of SNPs segregating. We demonstrate the potential of this MAGIC resource by identifying a highly diagnostic marker for the morphological character "awn presence/absence" and independently validate it in an association-mapping panel. These analyses show this large, diverse, and highly recombined MAGIC population to be a powerful resource for the genetic dissection of target traits in wheat, and it is well-placed to efficiently exploit ongoing advances in phenomics and genomics. Genetic marker and trait data, together with instructions for access to seed, are available at http://www.niab.com/MAGIC/.

Original languageEnglish
Pages (from-to)1603-10
Number of pages8
JournalG3: Genes, Genomes, Genetics
Volume4
Issue number9
DOIs
Publication statusPrint publication - 18 Sep 2014
Externally publishedYes

Bibliographical note

Copyright © 2014 Mackay et al.

Keywords

  • Chromosome Mapping
  • Crosses, Genetic
  • DNA, Plant/genetics
  • Disease Resistance
  • Flowers/physiology
  • Gene Frequency
  • Genome, Plant
  • Genotype
  • Linkage Disequilibrium
  • Phenotype
  • Plant Leaves/genetics
  • Polymorphism, Single Nucleotide
  • Quantitative Trait Loci
  • Reproducibility of Results
  • Seedlings/genetics
  • Triticum/genetics

Fingerprint Dive into the research topics of 'An eight-parent multiparent advanced generation inter-cross population for winter-sown wheat: creation, properties, and validation'. Together they form a unique fingerprint.

Cite this