Analysis of Arabidopsis genome sequence reveals a large new gene family in plants

J. P. Ride, E. M. Davies, F. C.H. Franklin, D. F. Marshall

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

A detailed analysis of the currently available Arabidopsis thaliana genomic sequence has revealed the presence of a large number of open reading frames with homology to the stigmatic self-incompatibility (S) genes of Papaver rhoeas. The products of these potential genes are all predicted to be relatively small, basic, secreted proteins with similar predicted secondary structures. We have named these potential genes SPH (S-protein homologues). Their presence appears to have been largely missed by the prediction methods currently used on the genomic sequence. Equivalent homologues could not be detected in the human, microbial, Drosophila or C. elegans genomic databases, suggesting a function specific to plants. Preliminary RT-PCR analysis indicates that at least two members of the family (SPH1, SPH8) are expressed, with expression being greatest in floral tissues. The gene family may total more than 100 members, and its discovery not only illustrates the importance of the genome sequencing efforts, but also indicates the extent of information which remains hidden after the initial trawl for potential genes.
Original languageEnglish
Pages (from-to)927-932
Number of pages6
JournalPlant Molecular Biology
Volume39
Issue number5
DOIs
Publication statusPrint publication - Mar 1999
Externally publishedYes

Fingerprint

Arabidopsis
Genome
genome
Genes
Rheiformes
genes
Papaver
Papaver rhoeas
genomics
Open Reading Frames
Drosophila
Proteins
Databases
open reading frames
Polymerase Chain Reaction
Arabidopsis thaliana
proteins
reverse transcriptase polymerase chain reaction
prediction
methodology

Keywords

  • Arabidopsis thaliana
  • Papaver rhoeas
  • Poppy
  • S-protein
  • Self-incompatibility

Cite this

Ride, J. P. ; Davies, E. M. ; Franklin, F. C.H. ; Marshall, D. F. / Analysis of Arabidopsis genome sequence reveals a large new gene family in plants. In: Plant Molecular Biology. 1999 ; Vol. 39, No. 5. pp. 927-932.
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Analysis of Arabidopsis genome sequence reveals a large new gene family in plants. / Ride, J. P.; Davies, E. M.; Franklin, F. C.H.; Marshall, D. F.

In: Plant Molecular Biology, Vol. 39, No. 5, 03.1999, p. 927-932.

Research output: Contribution to journalArticle

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