Analysis of temporal fecal microbiota dynamics in weaner pigs with and without exposure to enterotoxigenic Escherichia coli

J Pollock, DL Gally, L Glendinning, R Tiwari, MR Hutchings, JGM Houdijk

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)
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Abstract

The primary aim of this work was to study potential effects of sub-clinical enterotoxigenic Escherichia coli (ETEC) exposure on porcine fecal microbiota composition, with a secondary aim of profiling temporal shifts in bacterial communities over the weaning transition period. 16S rRNA gene metabarcoding and quantitative PCR (qPCR) were used to profile the fecal microbiota and quantify ETEC excretion in the feces, respectively. Temporal shifts in fecal microbiota structure and stability were observed across the immediate post-weaning period (P < 0.05), including significant shifts in the relative levels of specific bacterial phylotypes (P < 0.05). ETEC exposure did not change the fecal microbiota structure (P > 0.05), but significant variations in fecal community structure and stability were linked to variations in ETEC excretion level at particular time points (P < 0.05). In this study, marked temporal changes in microbiota structure and stability were evident over the short weaning transition period, with a relationship between ETEC excretion level and fecal microbiota composition being observed. This study has provided a detailed analysis of fecal microbiota dynamics in the pig, which should help to inform the development of novel management strategies for enteric disorders based on an improved understanding of microbial populations during the challenging post-weaning period.
Original languageEnglish
Pages (from-to)3777 - 3790
Number of pages14
JournalJournal of Animal Science
Volume96
Issue number9
Early online date3 Jul 2018
DOIs
Publication statusFirst published - 3 Jul 2018

Fingerprint

Enterotoxigenic Escherichia coli
Microbiota
Swine
Weaning
rRNA Genes
Feces
Polymerase Chain Reaction
Population

Bibliographical note

1025227
1032205

Keywords

  • 16S rRNA gene
  • ETEC
  • Metabarcoding
  • Microbiome
  • Weaner pigs

Cite this

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title = "Analysis of temporal fecal microbiota dynamics in weaner pigs with and without exposure to enterotoxigenic Escherichia coli",
abstract = "The primary aim of this work was to study potential effects of sub-clinical enterotoxigenic Escherichia coli (ETEC) exposure on porcine fecal microbiota composition, with a secondary aim of profiling temporal shifts in bacterial communities over the weaning transition period. 16S rRNA gene metabarcoding and quantitative PCR (qPCR) were used to profile the fecal microbiota and quantify ETEC excretion in the feces, respectively. Temporal shifts in fecal microbiota structure and stability were observed across the immediate post-weaning period (P < 0.05), including significant shifts in the relative levels of specific bacterial phylotypes (P < 0.05). ETEC exposure did not change the fecal microbiota structure (P > 0.05), but significant variations in fecal community structure and stability were linked to variations in ETEC excretion level at particular time points (P < 0.05). In this study, marked temporal changes in microbiota structure and stability were evident over the short weaning transition period, with a relationship between ETEC excretion level and fecal microbiota composition being observed. This study has provided a detailed analysis of fecal microbiota dynamics in the pig, which should help to inform the development of novel management strategies for enteric disorders based on an improved understanding of microbial populations during the challenging post-weaning period.",
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Analysis of temporal fecal microbiota dynamics in weaner pigs with and without exposure to enterotoxigenic Escherichia coli. / Pollock, J; Gally, DL; Glendinning, L; Tiwari, R; Hutchings, MR; Houdijk, JGM.

In: Journal of Animal Science, Vol. 96, No. 9, 03.07.2018, p. 3777 - 3790.

Research output: Contribution to journalArticleResearchpeer-review

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