Analysis of the genetic diversity of ovine herpesvirus 2 in samples from livestock with malignant catarrhal fever

George C. Russell*, Sandra F. Scholes, David F. Twomey, Ann E. Courtenay, Dawn M. Grant, Bruce Lamond, David Norris, Kim Willoughby, David M. Haig, James P. Stewart

*Corresponding author for this work

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In order to define better virus isolates from animals with malignant catarrhal fever (MCF), segments of three genes of ovine herpesvirus-2 were amplified from diagnostic samples representing MCF cases with a range of clinical presentations in cattle, including head and eye, alimentary and neurological. The variation within each gene segment was estimated by DNA sequencing, which confirmed that the newly-annotated Ov9.5 gene was significantly more polymorphic than either of the other loci tested (segments of ORF50 and ORF75), with alleles that differed at over 60% of nucleotide positions. Despite this, the nine Ov9.5 alleles characterised had identical predicted splicing patterns and could be translated into Ov9.5 polypeptides with at least 49% amino acid identity. This multi-locus approach has potential for use in epidemiological studies and in charactering chains of infection. However there was no association between specific variants of OvHV-2 and the clinical/pathological presentation of MCF in the cattle analysed.

Original languageEnglish
Pages (from-to)63-71
Number of pages9
JournalVeterinary Microbiology
Volume172
Issue number1-2
Early online date28 Apr 2014
DOIs
Publication statusPrint publication - 6 Aug 2014
Externally publishedYes

Keywords

  • Inflammatory disease
  • Malignant catarrhal fever
  • Multi-locus Sequence typing
  • Polymorphism
  • Sequence analysis

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    Russell, G. C., Scholes, S. F., Twomey, D. F., Courtenay, A. E., Grant, D. M., Lamond, B., Norris, D., Willoughby, K., Haig, D. M., & Stewart, J. P. (2014). Analysis of the genetic diversity of ovine herpesvirus 2 in samples from livestock with malignant catarrhal fever. Veterinary Microbiology, 172(1-2), 63-71. https://doi.org/10.1016/j.vetmic.2014.04.011