Antimicrobial biosynthetic potential and genetic diversity of endophytic actinomycetes associated with medicinal plants

Anwesha Gohain, Animesh Gogoi, Rajal Debnath, Archana Yadav, Bhim P. Singh, Vijai K. Gupta, Rajeev Sharma, Ratul Saikia*

*Corresponding author for this work

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Endophytic actinomycetes are one of the primary groups that share symbiotic relationships with medicinal plants and are key reservoir of biologically active compounds. In this study, six selective medicinal plants were targeted for the first time for endophytic actinomycetes isolation from Gibbon Wild Life Sanctuary, Assam, India, during winter and summer and 76 isolates were obtained. The isolates were found to be prevalent in roots followed by stem and leaves. 16S rRNA gene sequence analysis revealed 16 genera, including rare genera, Verrucosispora, Isoptericola and Kytococcus, which have never been previously reported as endophytic. The genus Streptomyces (66%) was dominant in both seasons. Shannon's diversity index showed that Azadirachta indica (1.49), Rauwolfia serpentina (1.43) and Emblica officinalis (1.24) were relatively good habitat for endophytic actinomycetes. Antimicrobial strains showed prevalence of polyketide synthase (PKS) type-II (85%) followed by PKS type-I (14%) encoded in the genomes. Expression studies showed 12-fold upregulation of PKSII gene in seventh day of incubation for Streptomyces antibioticus (EAAG90). Our results emphasize that the actinomycetes assemblages within plant tissue exhibited biosynthetic systems encoding for important biologically active compounds.

Original languageEnglish
Article numberfnv158
JournalFEMS Microbiology Letters
Volume362
Issue number19
Early online date7 Sep 2015
DOIs
Publication statusPrint publication - Oct 2015
Externally publishedYes

Keywords

  • 16S rRNA gene
  • biosynthetic gene
  • Endophytic actinomycetes
  • polyketide synthase

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