Arcanobacterium bovis sp. nov., isolated from the milk of a cow with mastitis

Osama Sammra, G Foster, Abdulwahed Ahmed Hassan, Mazen Alssahen, Christoph Laemmler, Stephanie Glaeser, Peter Kaempfer, Hans-Juergen Busse, Maria Borowiak, Burkhard Malorny, Catriona Ritchie, Ellen Prenger-Berninghoff, Amir Abdulmawjood

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Abstract

A polyphasic taxonomic study was performed on an unidentified Arcanobacterium-like Gram-stain positive bacterium designated strain C605018/01/1ᵀ isolated from a milk sample collected from the udder of a cow at post mortem. Comparative 16S rRNA gene sequencing showed that the bacterium belonged to the genus Arcanobacterium and was most closely related to the type strain of Arcanobacterium pluranimalium (99.76 %); sequence similarities to all other Arcanobacterium species were below 97%. The wet-lab DNA-DNA hybridization values among strain C605018/01/1ᵀ and A. pluranimalium DSM 13483ᵀ were low, 16.9 % (reciprocal: 49.8 %).
Pertaining to the whole genome sequence with a total length of 2.02 Mb and 1654 protein counts, the novel strain C605018/01/01T displayed a G+C content of 51.6 % mol%.
The presence of the major menaquinone MK-9(H4) supported the affiliation of this strain to the genus Arcanobacterium. The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol-mannoside and an unidentified glycolipid and aminophospholipids.
Based on these tests it is proposed that strain C605018/01/1ᵀ should be classified as the novel species Arcanbacterium bovis sp. nov. The type strain of Arcanobacterium bovis is C605018/01/1ᵀ (CCUG 45425T = DSM 107286ᵀ = BCCM/LMG 30783ᵀ)
Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Early online date26 Jun 2020
DOIs
Publication statusFirst published - 26 Jun 2020

Keywords

  • Arcanobacterium
  • Arcanobacterium bovis sp. nov.
  • Gram-stain-positive
  • genome
  • milk

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    Sammra, O., Foster, G., Ahmed Hassan, A., Alssahen, M., Laemmler, C., Glaeser, S., Kaempfer, P., Busse, H-J., Borowiak, M., Malorny, B., Ritchie, C., Prenger-Berninghoff, E., & Abdulmawjood, A. (2020). Arcanobacterium bovis sp. nov., isolated from the milk of a cow with mastitis. International Journal of Systematic and Evolutionary Microbiology, 1-6. https://doi.org/10.1099/ijsem.0.004230