Archaeal abundance in post-mortem ruminal digesta may help predict methane emissions from beef cattle

RJ Wallace, JA Rooke, C-A Duthie, JJ Hyslop, DW Ross, N McKain, S Motta de Souza, TJ Snelling, A Waterhouse, R Roehe

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Methane produced from 35 Aberdeen-Angus and 33 Limousin cross steers was measured in respiration chambers. Each group was split to receive either a medium- or high-concentrate diet. Ruminal digesta samples were subsequently removed to investigate correlations between methane emissions and the rumen microbial community, as measured by qPCR of 16S or 18S rRNA genes. Diet had the greatest influence on methane emissions. The high-concentrate diet resulted in lower methane emissions (P , 0.001) than the medium-concentrate diet. Methane was correlated, irrespective of breed, with the abundance of archaea (R 5 0.39), bacteria (20.47), protozoa (0.45), Bacteroidetes (20.37) and Clostridium Cluster XIVa (20.35). The archaea5bacteria ratio provided a stronger correlation (0.49). A similar correlation was found with digesta samples taken 2–3 weeks later at slaughter. This finding could help enable greenhouse gas emissions of large animal cohorts to be predicted from samples taken conveniently in the abattoir.
Original languageEnglish
Pages (from-to)2 - 5
Number of pages4
JournalScientific Reports
Volume4
DOIs
Publication statusFirst published - 2014

Bibliographical note

1023378
1023322

Keywords

  • Genetic models
  • Metabolomics
  • Microbial ecology
  • Microbiology techniques

Fingerprint Dive into the research topics of 'Archaeal abundance in post-mortem ruminal digesta may help predict methane emissions from beef cattle'. Together they form a unique fingerprint.

  • Profiles

    No photo of Carol-Anne Duthie

    Carol-Anne Duthie

    Person: Academic contract that is research only

    No photo of Rainer Roehe

    Cite this

    Wallace, RJ., Rooke, JA., Duthie, C-A., Hyslop, JJ., Ross, DW., McKain, N., Motta de Souza, S., Snelling, TJ., Waterhouse, A., & Roehe, R. (2014). Archaeal abundance in post-mortem ruminal digesta may help predict methane emissions from beef cattle. Scientific Reports, 4, 2 - 5. https://doi.org/10.1038/srep05892