Assessment of a bacterial 6-phytase in the diets of broiler chickens

OA Olukosi, C Kong, F Fru-Nji, KM Ajuwon, O Adeola

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Abstract

Two 21-d broiler experiments were conducted to assess the efficacy of a bacterial 6-phytase expressed in Aspergillus oryzae on growth performance, nutrient utilization, and intestinal molecular markers. Two hundred forty birds in 5 treatments (experiment 1) or 256 birds in 4 treatments (experiment 2) were used. The treatments included a negative control diet that was marginally deficient in P (NC) or NC plus tricalcium phosphate, 500, 1,000, or 2,000 phytase units/kg (experiment 1), and NC or NC plus monocalcium phosphate, 500 or 1,000 phytase units/kg (experiment 2). In both experiments, excreta were collected on d 19 to 21, whereas birds and feed were weighed and ileal digesta collected on d 21. For experiment 1, mucosa scraping was collected from the duodenum, jejunum, and ileum from all birds for quantification of expression level of gut level inflammatory cytokines, Toll-like receptors, and phosphate transporter (NaPi-IIb). In both experiments, tricalcium phosphate, monocalcium phosphate, and phytase supplementation improved (P < 0.05) weight gain and percentage tibia ash. Phosphorus and Ca retention and phytic acid disappearance improved (P < 0.05) with phytase supplementation (experiment 1) and there was an increase (P < 0.01) in Ca and P retention in response to phytase supplementation (experiment 2). Diets did not affect the expression of gut level cytokines, Toll-like receptors, or the mucin gene. Phytase supplementation tended to decrease (P < 0.10) the expression of NaPi-IIb. It was concluded from these studies that the bacterial 6-phytase was effective in enhancing growth of broilers receiving low-P diets as well as in increasing efficiency of P utilization and phytic acid degradation.
Original languageEnglish
Pages (from-to)2101 - 2108
Number of pages8
JournalPoultry Science
Volume92
Issue number8
DOIs
Publication statusPrint publication - Aug 2013

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Bibliographical note

2064639

Keywords

  • Broiler
  • Growth
  • Nutrient utilization
  • Phytase

Cite this

Olukosi, OA., Kong, C., Fru-Nji, F., Ajuwon, KM., & Adeola, O. (2013). Assessment of a bacterial 6-phytase in the diets of broiler chickens. Poultry Science, 92(8), 2101 - 2108. https://doi.org/10.3382/ps.2012-03005