Assessment of ciliates in the sheep rumen by DGGE

M Regensbogenova, P Pristas, P Javorsky, S Y Moon-van der Staay, G W M van der Staay, J H P Hackstein, C J Newbold, N R McEwan

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

AIMS: This work was carried out to develop a rapid molecular profiling technique to screen ciliate populations in the rumen of sheep.

METHODS AND RESULTS: DGGE was used to study the ciliate diversity in the rumen of sheep. There was considerable variation between sheep which were co-housed, and fed the same diet. However, no difference in the major banding patterns was detected, when samples were collected from a single sheep sampled at different points. Following dietary changes, use of a pair-wise comparison of lanes, demonstrated that although there was still diversity between the ciliate population of sheep, the effects as a result of dietary changes were greater.

CONCLUSIONS: The technique generated molecular profiles which are sufficiently different to allow comparison between samples, and to permit molecular ecological studies on the rumen ciliate population.

SIGNIFICANCE AND IMPACT OF THE STUDY: The outcome of this study means that ciliate diversity in the rumen may now be studied by those unfamiliar with morphological identification of these organisms.

Original languageEnglish
Pages (from-to)144-7
Number of pages4
JournalLetters in Applied Microbiology
Volume39
Issue number2
DOIs
Publication statusPrint publication - 2004
Externally publishedYes

Fingerprint

Rumen
Sheep
Population
Outcome Assessment (Health Care)
Diet

Keywords

  • Animal Feed
  • Animals
  • Ciliophora/genetics
  • DNA Fingerprinting/methods
  • DNA, Ribosomal/analysis
  • Electrophoresis, Polyacrylamide Gel
  • Nucleic Acid Denaturation/genetics
  • Polymerase Chain Reaction
  • RNA, Ribosomal, 18S/genetics
  • Rumen/metabolism
  • Sheep/metabolism

Cite this

Regensbogenova, M., Pristas, P., Javorsky, P., Moon-van der Staay, S. Y., van der Staay, G. W. M., Hackstein, J. H. P., ... McEwan, N. R. (2004). Assessment of ciliates in the sheep rumen by DGGE. Letters in Applied Microbiology, 39(2), 144-7. https://doi.org/10.1111/j.1472-765X.2004.01542.x
Regensbogenova, M ; Pristas, P ; Javorsky, P ; Moon-van der Staay, S Y ; van der Staay, G W M ; Hackstein, J H P ; Newbold, C J ; McEwan, N R. / Assessment of ciliates in the sheep rumen by DGGE. In: Letters in Applied Microbiology. 2004 ; Vol. 39, No. 2. pp. 144-7.
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Regensbogenova, M, Pristas, P, Javorsky, P, Moon-van der Staay, SY, van der Staay, GWM, Hackstein, JHP, Newbold, CJ & McEwan, NR 2004, 'Assessment of ciliates in the sheep rumen by DGGE', Letters in Applied Microbiology, vol. 39, no. 2, pp. 144-7. https://doi.org/10.1111/j.1472-765X.2004.01542.x

Assessment of ciliates in the sheep rumen by DGGE. / Regensbogenova, M; Pristas, P; Javorsky, P; Moon-van der Staay, S Y; van der Staay, G W M; Hackstein, J H P; Newbold, C J; McEwan, N R.

In: Letters in Applied Microbiology, Vol. 39, No. 2, 2004, p. 144-7.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Assessment of ciliates in the sheep rumen by DGGE

AU - Regensbogenova, M

AU - Pristas, P

AU - Javorsky, P

AU - Moon-van der Staay, S Y

AU - van der Staay, G W M

AU - Hackstein, J H P

AU - Newbold, C J

AU - McEwan, N R

PY - 2004

Y1 - 2004

N2 - AIMS: This work was carried out to develop a rapid molecular profiling technique to screen ciliate populations in the rumen of sheep.METHODS AND RESULTS: DGGE was used to study the ciliate diversity in the rumen of sheep. There was considerable variation between sheep which were co-housed, and fed the same diet. However, no difference in the major banding patterns was detected, when samples were collected from a single sheep sampled at different points. Following dietary changes, use of a pair-wise comparison of lanes, demonstrated that although there was still diversity between the ciliate population of sheep, the effects as a result of dietary changes were greater.CONCLUSIONS: The technique generated molecular profiles which are sufficiently different to allow comparison between samples, and to permit molecular ecological studies on the rumen ciliate population.SIGNIFICANCE AND IMPACT OF THE STUDY: The outcome of this study means that ciliate diversity in the rumen may now be studied by those unfamiliar with morphological identification of these organisms.

AB - AIMS: This work was carried out to develop a rapid molecular profiling technique to screen ciliate populations in the rumen of sheep.METHODS AND RESULTS: DGGE was used to study the ciliate diversity in the rumen of sheep. There was considerable variation between sheep which were co-housed, and fed the same diet. However, no difference in the major banding patterns was detected, when samples were collected from a single sheep sampled at different points. Following dietary changes, use of a pair-wise comparison of lanes, demonstrated that although there was still diversity between the ciliate population of sheep, the effects as a result of dietary changes were greater.CONCLUSIONS: The technique generated molecular profiles which are sufficiently different to allow comparison between samples, and to permit molecular ecological studies on the rumen ciliate population.SIGNIFICANCE AND IMPACT OF THE STUDY: The outcome of this study means that ciliate diversity in the rumen may now be studied by those unfamiliar with morphological identification of these organisms.

KW - Animal Feed

KW - Animals

KW - Ciliophora/genetics

KW - DNA Fingerprinting/methods

KW - DNA, Ribosomal/analysis

KW - Electrophoresis, Polyacrylamide Gel

KW - Nucleic Acid Denaturation/genetics

KW - Polymerase Chain Reaction

KW - RNA, Ribosomal, 18S/genetics

KW - Rumen/metabolism

KW - Sheep/metabolism

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VL - 39

SP - 144

EP - 147

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

SN - 0266-8254

IS - 2

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Regensbogenova M, Pristas P, Javorsky P, Moon-van der Staay SY, van der Staay GWM, Hackstein JHP et al. Assessment of ciliates in the sheep rumen by DGGE. Letters in Applied Microbiology. 2004;39(2):144-7. https://doi.org/10.1111/j.1472-765X.2004.01542.x