B chromosomes are associated with redistribution of genetic recombination towards lower recombination chromosomal regions in perennial ryegrass

J Harper, D Phillips, A Thomas, D Gasior, C Evans, W Powell, J King, I King, G Jenkins, I Armstead

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Abstract

Supernumerary ‘B’ chromosomes are non-essential components of the genome present in a range of plant and animal species—including many grasses. Within diploid and polyploid ryegrass and fescue species, including the forage grass perennial ryegrass (Lolium perenne L.), the presence of B chromosomes has been reported as influencing both chromosome pairing and chiasma frequencies. In this study, the effects of the presence/absence of B chromosomes on genetic recombination has been investigated through generating DArT (Diversity Arrays Technology) marker genetic maps for six perennial ryegrass diploid populations, the pollen parents of which contained either two B or zero B chromosomes. Through genetic and cytological analyses of these progeny and their parents, we have identified that, while overall cytological estimates of chiasma frequencies were significantly lower in pollen mother cells with two B chromosomes as compared with zero B chromosomes, the recombination frequencies within some marker intervals were actually increased, particularly for marker intervals in lower recombination regions of chromosomes, namely pericentromeric regions. Thus, in perennial ryegrass, the presence of two B chromosomes redistributed patterns of meiotic recombination in pollen mother cells in ways which could increase the range of allelic variation available to plant breeders.
Original languageEnglish
Pages (from-to)1861 - 1871
Number of pages11
JournalJournal of Experimental Botany
Volume69
Issue number8
Early online date21 Mar 2018
DOIs
Publication statusFirst published - 21 Mar 2018

Keywords

  • B chromosomes
  • Chiasma
  • Genetic mapping
  • Lolium perenne
  • Meiosis
  • Perennial ryegrass
  • Recombination

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