Bacterial community composition and diversity respond to nutrient amendment but not warming in a maritime Antarctic soil

Kevin K Newsham*, Binu M Tripathi, Ke Dong, Naomichi Yamamoto, Jonathan M Adams, David W Hopkins

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

A resumption of climate warming in maritime Antarctica, arising from continued greenhouse gas emissions to the atmosphere, is predicted to lead to further expansions of plant populations across the region, with consequent increases in nutrient inputs to soils. Here, we test the main and interactive effects of warming, applied with open top chambers (OTCs), and nutrient amendment with tryptic soy broth (TSB), an artificial growth substrate, on bacterial community composition and diversity using Illumina sequencing of 16S rRNA genes in soil from a field experiment in the southern maritime Antarctic. Substantial effects of TSB application on bacterial communities were identified after 49 months, including reduced diversity, altered phylogenetic community assembly processes, increased Proteobacteria-to-Acidobacteria ratios and significant divergence in community composition, notably increases in the relative abundances of the gram-positive genera Arthrobacter, Paeniglutamicibacter and Planococcus. Contrary to previous observations from other maritime Antarctic field warming experiments, we recorded no effects of warming with OTCs, or interactive effects of OTCs and TSB application, on bacterial community composition or diversity. Based on these findings, we conclude that further warming of the maritime Antarctic is unlikely to influence soil bacterial community composition or diversity directly, but that increased nutrient inputs arising from enhanced plant growth across the region may affect the composition of soil bacterial communities, with possible effects on ecosystem productivity.

Original languageEnglish
Pages (from-to)974-984
Number of pages11
JournalMicrobial Ecology
Volume78
Issue number4
Early online date15 Apr 2019
DOIs
Publication statusPrint publication - Nov 2019

Fingerprint

bacterial communities
community composition
warming
soil bacteria
open-top chamber
Planococcus (bacteria)
nutrient
nutrients
Acidobacteria
Arthrobacter
soil
Proteobacteria
greenhouse gas emissions
Antarctica
global warming
ribosomal RNA
plant growth
ecosystems
phylogeny
relative abundance

Keywords

  • Antarctica
  • Bacterial community composition
  • Climate warming
  • Gram-positive and gram-negative bacteria
  • Nutrient inputs
  • Proteobacteria-to-Acidobacteria ratio

Cite this

Newsham, Kevin K ; Tripathi, Binu M ; Dong, Ke ; Yamamoto, Naomichi ; Adams, Jonathan M ; Hopkins, David W. / Bacterial community composition and diversity respond to nutrient amendment but not warming in a maritime Antarctic soil. In: Microbial Ecology. 2019 ; Vol. 78, No. 4. pp. 974-984.
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Bacterial community composition and diversity respond to nutrient amendment but not warming in a maritime Antarctic soil. / Newsham, Kevin K; Tripathi, Binu M; Dong, Ke; Yamamoto, Naomichi; Adams, Jonathan M; Hopkins, David W.

In: Microbial Ecology, Vol. 78, No. 4, 11.2019, p. 974-984.

Research output: Contribution to journalArticle

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