Biocontrol of Fusarium wilt of Capsicum annuum by rhizospheric bacteria isolated from turmeric endowed with plant growth promotion and disease suppression potential

Ajit Kumar Passari, P. C. Lalsiamthari, Zothanpuia, Vincent Vineeth Leo, Vineet Kumar Mishra, Mukesh Kumar Yadav, Vijai Kumar Gupta, Bhim Pratap Singh*

*Corresponding author for this work

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In the present study, 129 rhizospheric bacteria isolated from Curcuma longa were screened for their antagonistic potential against six fungal phytopathogens. Among them, 32 isolates that showed significant antagonistic potential were screened for their in vitro plant growth promoting (PGP) traits. The identification of potential isolates was confirmed by 16S rRNA gene sequencing and results revealed Bacillus as the dominant genus followed by Staphylococcus, Pseudomonas, Sphingomonas and Achromobacter. Based on the antagonistic activity and PGP traits; two strains (BPSRB4 and BPSRB14), identified as Bacillus amyloliquefaciens, were further tested for their in vivo PGP and disease suppression potential on Capsicum annuum seedlings under greenhouse conditions. The results demonstrated that BPSRB4 and BPSR14 strains suppress fungal pathogen infection and promote plant growth. Further, the BPSRB4 strain was positive for the production of the phytohormone indole acetic acid (IAA) detected by thin layer chromatography (TLC). In addition, nitrogen fixation and plant growth promotion activity were also confirmed by amplification and sequencing of nitrogen fixation gene (nifH) and ACC (1-aminocyclopropane-1-carboxylate) deaminase (acdS) gene from strains BPSRB4 and BPSRB14. The present study demonstrated that the B. amyloliquefaciens strains BPSRB4 and BPSR14 possess antagonistic activity and PGP potential which could be explored for the development of biofertilizers and biocontrol agents for the growth of chilli seedlings.

Original languageEnglish
Pages (from-to)831-846
Number of pages16
JournalEuropean Journal of Plant Pathology
Volume150
Issue number4
Early online date23 Aug 2017
DOIs
Publication statusPrint publication - Apr 2018
Externally publishedYes

Keywords

  • 16S rDNA
  • Biocontrol
  • Curcuma Longa
  • Rhizospheric bacteria
  • Thin layer chromatography (TLC)

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