Biofabrication of zinc oxide nanoparticles with Syzygium aromaticum flower buds extract and finding its novel application in controlling the growth and mycotoxins of Fusarium graminearum

Thimappa Ramachandrappa Lakshmeesha, Naveen Kumar Kalagatur, Venkataramana Mudili, Chakrabhavi Dhananjaya Mohan, Shobith Rangappa, Bangari Daruka Prasad, Bagepalli Shivaram Ashwini, Abeer Hashem, Abdulaziz A. Alqarawi, Jahangir Ahmad Malik, Elsayed Fathi Abd-Allah, Vijai Kumar Gupta, Chandra Nayaka Siddaiah*, Siddapura Ramachandrappa Niranjana

*Corresponding author for this work

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Fusarium graminearum is a leading plant pathogen that causes Fusarium head blight, stalk rot, and Gibberella ear rot diseases in cereals and posing the immense threat to the microbiological safety of the food. Herein, we report the green synthesis of zinc oxide nanoparticles from Syzygium aromaticum (SaZnO NPs) flower bud extract by combustion method and investigated their application for controlling of growth and mycotoxins of F. graminearum. Formation of SaZnO NPs was confirmed by spectroscopic methods. The electron microscopic (SEM and TEM) analysis revealed the formation of triangular and hexagonal shaped SaZnO NPs with size range 30-40 nm. The synthesized SaZnO NPs reduced the growth and production of deoxynivalenol and zearalenone of F. graminearum in broth culture. Further analysis revealed that treatment of mycelia with SaZnO NPs resulted in the accumulation of ROS in the dose-dependent manner. Also, SaZnO NPs treatment enhanced lipid peroxidation, depleted ergosterol content, and caused detrimental damage to the membrane integrity of fungi. Moreover, SEM observations revealed that the presence of diverged micro-morphology (wrinkled, rough and shrank surface) in the macroconidia treated with SaZnO NPs. Taken together, SaZnO NPs may find a potential application in agriculture and food industries due to their potent antifungal activity.

Original languageEnglish
Article number1244
JournalFrontiers in Microbiology
Volume10
Issue numberJUN
Early online date12 Jun 2019
DOIs
Publication statusFirst published - 12 Jun 2019
Externally publishedYes

Keywords

  • Deoxynivalenol
  • Green synthesis
  • Mycotoxin
  • Zearalenone
  • Zinc oxide nanoparticles

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    Lakshmeesha, T. R., Kalagatur, N. K., Mudili, V., Mohan, C. D., Rangappa, S., Prasad, B. D., Ashwini, B. S., Hashem, A., Alqarawi, A. A., Malik, J. A., Abd-Allah, E. F., Gupta, V. K., Siddaiah, C. N., & Niranjana, S. R. (2019). Biofabrication of zinc oxide nanoparticles with Syzygium aromaticum flower buds extract and finding its novel application in controlling the growth and mycotoxins of Fusarium graminearum. Frontiers in Microbiology, 10(JUN), [1244]. https://doi.org/10.3389/fmicb.2019.01244