TY - JOUR
T1 - Biofabrication of zinc oxide nanoparticles with Syzygium aromaticum flower buds extract and finding its novel application in controlling the growth and mycotoxins of Fusarium graminearum
AU - Lakshmeesha, Thimappa Ramachandrappa
AU - Kalagatur, Naveen Kumar
AU - Mudili, Venkataramana
AU - Mohan, Chakrabhavi Dhananjaya
AU - Rangappa, Shobith
AU - Prasad, Bangari Daruka
AU - Ashwini, Bagepalli Shivaram
AU - Hashem, Abeer
AU - Alqarawi, Abdulaziz A.
AU - Malik, Jahangir Ahmad
AU - Abd-Allah, Elsayed Fathi
AU - Gupta, Vijai Kumar
AU - Siddaiah, Chandra Nayaka
AU - Niranjana, Siddapura Ramachandrappa
PY - 2019/6/12
Y1 - 2019/6/12
N2 - Fusarium graminearum is a leading plant pathogen that causes Fusarium head blight, stalk rot, and Gibberella ear rot diseases in cereals and posing the immense threat to the microbiological safety of the food. Herein, we report the green synthesis of zinc oxide nanoparticles from Syzygium aromaticum (SaZnO NPs) flower bud extract by combustion method and investigated their application for controlling of growth and mycotoxins of F. graminearum. Formation of SaZnO NPs was confirmed by spectroscopic methods. The electron microscopic (SEM and TEM) analysis revealed the formation of triangular and hexagonal shaped SaZnO NPs with size range 30-40 nm. The synthesized SaZnO NPs reduced the growth and production of deoxynivalenol and zearalenone of F. graminearum in broth culture. Further analysis revealed that treatment of mycelia with SaZnO NPs resulted in the accumulation of ROS in the dose-dependent manner. Also, SaZnO NPs treatment enhanced lipid peroxidation, depleted ergosterol content, and caused detrimental damage to the membrane integrity of fungi. Moreover, SEM observations revealed that the presence of diverged micro-morphology (wrinkled, rough and shrank surface) in the macroconidia treated with SaZnO NPs. Taken together, SaZnO NPs may find a potential application in agriculture and food industries due to their potent antifungal activity.
AB - Fusarium graminearum is a leading plant pathogen that causes Fusarium head blight, stalk rot, and Gibberella ear rot diseases in cereals and posing the immense threat to the microbiological safety of the food. Herein, we report the green synthesis of zinc oxide nanoparticles from Syzygium aromaticum (SaZnO NPs) flower bud extract by combustion method and investigated their application for controlling of growth and mycotoxins of F. graminearum. Formation of SaZnO NPs was confirmed by spectroscopic methods. The electron microscopic (SEM and TEM) analysis revealed the formation of triangular and hexagonal shaped SaZnO NPs with size range 30-40 nm. The synthesized SaZnO NPs reduced the growth and production of deoxynivalenol and zearalenone of F. graminearum in broth culture. Further analysis revealed that treatment of mycelia with SaZnO NPs resulted in the accumulation of ROS in the dose-dependent manner. Also, SaZnO NPs treatment enhanced lipid peroxidation, depleted ergosterol content, and caused detrimental damage to the membrane integrity of fungi. Moreover, SEM observations revealed that the presence of diverged micro-morphology (wrinkled, rough and shrank surface) in the macroconidia treated with SaZnO NPs. Taken together, SaZnO NPs may find a potential application in agriculture and food industries due to their potent antifungal activity.
KW - Deoxynivalenol
KW - Green synthesis
KW - Mycotoxin
KW - Zearalenone
KW - Zinc oxide nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=85069173075&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2019.01244
DO - 10.3389/fmicb.2019.01244
M3 - Article
C2 - 31249558
AN - SCOPUS:85069173075
SN - 1664-302X
VL - 10
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
IS - JUN
M1 - 1244
ER -