Biohydrogen production via integrated sequential fermentation using magnetite nanoparticles treated crude enzyme to hydrolyze sugarcane bagasse

Neha Srivastava, Alaa Alhazmi, Akbar Mohammad, Shafiul Haque, Manish Srivastava, Dan Bahadur Pal, Rajeev Singh, P. K. Mishra, Dai Viet N. Vo, Taeho Yoon, Vijai Kumar Gupta*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

This study presents a potential approach to enhance integrated sequential biohydrogen production from waste biomass using magnetite nanoparticle (Fe3O4 NPs) which is synthesized through waste seeds of Syzygium cumini. Consequences of 0.5% Fe3O4 NPs have been investigated on the thermal and pH stability of fungal crude cellulase. It is noticed that Fe3O4 NPs treated enzyme and control exhibits 100% activity in the temperature range of 45–60 °C and 45–55 °C, respectively. Moreover, Fe3O4 NPs treated enzyme showed extended thermal stability in the temperature range of 50–60 °C up to 12 h. Beside this, Fe3O4 NPs treated enzyme possesses 100% stability in the pH range of 5.0–7.0 whereas control exhibited only at pH 6.0. Enzymatic hydrolysis via Fe3O4 NPs treated enzyme has been employed which produces ∼68.0 g/L reducing sugars from sugarcane bagasse. Subsequently, sugar hydrolyzate has been utilized as substrate in the sequential integrated fermentation that produces ∼3427.0 mL/L cumulative hydrogen after 408 h. This approach may have potential for the pilot scale production of biohydrogen from waste biomass at low-cost in an eco-friendly manner.

Original languageEnglish
JournalInternational Journal of Hydrogen Energy
Early online date20 Sep 2021
DOIs
Publication statusFirst published - 20 Sep 2021

Keywords

  • Biohydrogen
  • Dark-fermentation
  • Green synthesis
  • Iron oxide nanoparticles
  • Photo-fermentation
  • Reducing sugars

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