Biologically derived copper oxide-based nanocatalyst using Moringa oleifera leaves and its applications in hydrolytic enzymes and biohydrogen production

Neha Srivastava, Rajeev Singh, Irfan Ahmad, Mohammed Asiri, Subhash C Tripathi, Ashutosh Kumar Rai, P K Mishra, Vijai Kumar Gupta

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Due to the limited availability of fossil fuels, pollution causing serious environmental issues, and their continuously rising price, the development of low-cost efficient enzymes and their implementation in biomass-based bioenergy industries are highly demanded. In the present work, phytogenic fabrication of copper oxide based nanocatalyst has been performed using moringa leaves and has been characterized using different techniques. Herein, the impact of different dosages of as-prepared nanocatalyst on fungal co-cultured cellulolytic enzyme production under co-substrate fermentation using wheat straw and sugarcane bagasse in 4:2 ratios in solid state fermentation (SSF) has been investigated. An optimal concentration of 25 ppm of nanocatalyst influenced the production of 32 IU/gds of enzyme, which showed thermal stability at 70 °C for 15 h. Additionally, enzymatic bioconversion of rice husk at 70 °C librated 41 g/L of total reducing sugars, which led to the production of 2390 mL/L of cumulative H 2 in 120 h.

Original languageEnglish
Article number128847
Number of pages5
JournalBioresource Technology
Volume376
Issue number128847
Early online date22 Mar 2023
DOIs
Publication statusPrint publication - May 2023

Bibliographical note

Copyright © 2023 Elsevier Ltd. All rights reserved.

Keywords

  • cellulases
  • Lignocellulosic biomass
  • copper oxide nanoparticles
  • biohydrogen
  • phytosynthesis
  • Biohydrogen
  • Cellulases
  • Phytosynthesis
  • Copper oxide nanoparticles

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