Body protein reserves sustained maternal performances in early lactation but dietary protein was necessary to maintain performance and immune responses to Nippostrongylus brasiliensis in lactating rats

A Masuda, JGM Houdijk, JE Allen, S Athanasiadou

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Abstract

Background: It has been shown that dietary protein supplementation during lactation boosts immunity in Nippostrongylus brasiliensis-infected periparturient rats. It is not known whether body protein reserves accumulated during gestation have a similar effect during lactation. Objective: This study aimed to quantify the impact of body protein reserves and dietary protein supplementation on maternal performances and immune responses to N. brasiliensis during lactation. Methods: Multiparous female Sprague-Dawley rats were given a primary infection of N. brasiliensis prior to mating and restrictedly fed either 60 g (Lge) or 210 g (Hge) crude protein (CP) per kg dry matter (DM) until parturition. Parturition onwards, dams were restrictedly fed either 100 g (Lla) or 300 g (Hla) CP per kg DM, generating 4 different dietary treatments. A subset of rats was sampled before parturition; post-parturition, dams were secondary infected with N. brasiliensis and samples were collected at day 5 and 11 post parturition. Results: Maternal performance until parturition, as measured by pup weight, was better in Hge rats compared to Lge (Lge 4.84 g, Hge 6.15 g, S.E.D. 0.19). On day 11, pup weight of dams with reduced protein reserves receiving protein during lactation (Lge-Hla, 20.28 g) was higher compared to their counterparts from Hge-Lla dams (17.88 g, S.E.D. 0.92). Worm counts were significantly different between Lge-Lla (253; 95% CI 124 to 382) and Hge-Hla fed dams (87; 95% CI 22 to 104) on day 11 (P=0.024). Expression of splenic Il13 and Alox15 was significantly higher (P<0.05) in Hge-Hla dams compared to Lge-Lla on day 5. Conclusions: Although protein reserves were adequate to maintain maternal performances in the early stage of lactation in dams infected with N. brasiliensis. they were not adequate to maintain maternal performances and effective immune responses at the later stages. Dietary protein supplementation was required to achieve this.
Original languageEnglish
Pages (from-to)1638 - 1646
Number of pages9
JournalJournal of Nutrition
Volume148
Issue number10
Early online date10 Sep 2018
DOIs
Publication statusFirst published - 10 Sep 2018

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Nippostrongylus
Dietary Proteins
Lactation
Mothers
Parturition
Dietary Supplements
Proteins
Weights and Measures
Interleukin-13
Sprague Dawley Rats
Immunity
Pregnancy
Infection

Bibliographical note

1026516
1024944

Keywords

  • Dietary protein
  • Gastrointestinal nematodes
  • Lactation
  • Nippostrongylus brasiliensis
  • Nutrition
  • Periparturient relaxation of immunity

Cite this

@article{ef89c4bbf3314c3e9dc76f1a3b2b863f,
title = "Body protein reserves sustained maternal performances in early lactation but dietary protein was necessary to maintain performance and immune responses to Nippostrongylus brasiliensis in lactating rats",
abstract = "Background: It has been shown that dietary protein supplementation during lactation boosts immunity in Nippostrongylus brasiliensis-infected periparturient rats. It is not known whether body protein reserves accumulated during gestation have a similar effect during lactation. Objective: This study aimed to quantify the impact of body protein reserves and dietary protein supplementation on maternal performances and immune responses to N. brasiliensis during lactation. Methods: Multiparous female Sprague-Dawley rats were given a primary infection of N. brasiliensis prior to mating and restrictedly fed either 60 g (Lge) or 210 g (Hge) crude protein (CP) per kg dry matter (DM) until parturition. Parturition onwards, dams were restrictedly fed either 100 g (Lla) or 300 g (Hla) CP per kg DM, generating 4 different dietary treatments. A subset of rats was sampled before parturition; post-parturition, dams were secondary infected with N. brasiliensis and samples were collected at day 5 and 11 post parturition. Results: Maternal performance until parturition, as measured by pup weight, was better in Hge rats compared to Lge (Lge 4.84 g, Hge 6.15 g, S.E.D. 0.19). On day 11, pup weight of dams with reduced protein reserves receiving protein during lactation (Lge-Hla, 20.28 g) was higher compared to their counterparts from Hge-Lla dams (17.88 g, S.E.D. 0.92). Worm counts were significantly different between Lge-Lla (253; 95{\%} CI 124 to 382) and Hge-Hla fed dams (87; 95{\%} CI 22 to 104) on day 11 (P=0.024). Expression of splenic Il13 and Alox15 was significantly higher (P<0.05) in Hge-Hla dams compared to Lge-Lla on day 5. Conclusions: Although protein reserves were adequate to maintain maternal performances in the early stage of lactation in dams infected with N. brasiliensis. they were not adequate to maintain maternal performances and effective immune responses at the later stages. Dietary protein supplementation was required to achieve this.",
keywords = "Dietary protein, Gastrointestinal nematodes, Lactation, Nippostrongylus brasiliensis, Nutrition, Periparturient relaxation of immunity",
author = "A Masuda and JGM Houdijk and JE Allen and S Athanasiadou",
note = "1026516 1024944",
year = "2018",
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doi = "10.1093/jn/nxy133",
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volume = "148",
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TY - JOUR

T1 - Body protein reserves sustained maternal performances in early lactation but dietary protein was necessary to maintain performance and immune responses to Nippostrongylus brasiliensis in lactating rats

AU - Masuda, A

AU - Houdijk, JGM

AU - Allen, JE

AU - Athanasiadou, S

N1 - 1026516 1024944

PY - 2018/9/10

Y1 - 2018/9/10

N2 - Background: It has been shown that dietary protein supplementation during lactation boosts immunity in Nippostrongylus brasiliensis-infected periparturient rats. It is not known whether body protein reserves accumulated during gestation have a similar effect during lactation. Objective: This study aimed to quantify the impact of body protein reserves and dietary protein supplementation on maternal performances and immune responses to N. brasiliensis during lactation. Methods: Multiparous female Sprague-Dawley rats were given a primary infection of N. brasiliensis prior to mating and restrictedly fed either 60 g (Lge) or 210 g (Hge) crude protein (CP) per kg dry matter (DM) until parturition. Parturition onwards, dams were restrictedly fed either 100 g (Lla) or 300 g (Hla) CP per kg DM, generating 4 different dietary treatments. A subset of rats was sampled before parturition; post-parturition, dams were secondary infected with N. brasiliensis and samples were collected at day 5 and 11 post parturition. Results: Maternal performance until parturition, as measured by pup weight, was better in Hge rats compared to Lge (Lge 4.84 g, Hge 6.15 g, S.E.D. 0.19). On day 11, pup weight of dams with reduced protein reserves receiving protein during lactation (Lge-Hla, 20.28 g) was higher compared to their counterparts from Hge-Lla dams (17.88 g, S.E.D. 0.92). Worm counts were significantly different between Lge-Lla (253; 95% CI 124 to 382) and Hge-Hla fed dams (87; 95% CI 22 to 104) on day 11 (P=0.024). Expression of splenic Il13 and Alox15 was significantly higher (P<0.05) in Hge-Hla dams compared to Lge-Lla on day 5. Conclusions: Although protein reserves were adequate to maintain maternal performances in the early stage of lactation in dams infected with N. brasiliensis. they were not adequate to maintain maternal performances and effective immune responses at the later stages. Dietary protein supplementation was required to achieve this.

AB - Background: It has been shown that dietary protein supplementation during lactation boosts immunity in Nippostrongylus brasiliensis-infected periparturient rats. It is not known whether body protein reserves accumulated during gestation have a similar effect during lactation. Objective: This study aimed to quantify the impact of body protein reserves and dietary protein supplementation on maternal performances and immune responses to N. brasiliensis during lactation. Methods: Multiparous female Sprague-Dawley rats were given a primary infection of N. brasiliensis prior to mating and restrictedly fed either 60 g (Lge) or 210 g (Hge) crude protein (CP) per kg dry matter (DM) until parturition. Parturition onwards, dams were restrictedly fed either 100 g (Lla) or 300 g (Hla) CP per kg DM, generating 4 different dietary treatments. A subset of rats was sampled before parturition; post-parturition, dams were secondary infected with N. brasiliensis and samples were collected at day 5 and 11 post parturition. Results: Maternal performance until parturition, as measured by pup weight, was better in Hge rats compared to Lge (Lge 4.84 g, Hge 6.15 g, S.E.D. 0.19). On day 11, pup weight of dams with reduced protein reserves receiving protein during lactation (Lge-Hla, 20.28 g) was higher compared to their counterparts from Hge-Lla dams (17.88 g, S.E.D. 0.92). Worm counts were significantly different between Lge-Lla (253; 95% CI 124 to 382) and Hge-Hla fed dams (87; 95% CI 22 to 104) on day 11 (P=0.024). Expression of splenic Il13 and Alox15 was significantly higher (P<0.05) in Hge-Hla dams compared to Lge-Lla on day 5. Conclusions: Although protein reserves were adequate to maintain maternal performances in the early stage of lactation in dams infected with N. brasiliensis. they were not adequate to maintain maternal performances and effective immune responses at the later stages. Dietary protein supplementation was required to achieve this.

KW - Dietary protein

KW - Gastrointestinal nematodes

KW - Lactation

KW - Nippostrongylus brasiliensis

KW - Nutrition

KW - Periparturient relaxation of immunity

UR - http://www.mendeley.com/research/body-protein-reserves-sustain-maternal-performance-early-lactation-dietary-protein-necessary-maintai

U2 - 10.1093/jn/nxy133

DO - 10.1093/jn/nxy133

M3 - Article

C2 - 30204917

VL - 148

SP - 1638

EP - 1646

JO - Journal of Nutrition

JF - Journal of Nutrition

SN - 0022-3166

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