Carbon transformations during decomposition of different components of plant leaves in soil

E. A. Webster*, J. A. Chudek, D. W. Hopkins

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

64 Citations (Scopus)

Abstract

We investigated the effect of lime addition to an upland organic soil on the decomposition of Lolium perenne leaves and isolated fractions of L. perenne leaves in a laboratory experiment lasting 75 d. The L. perenne plants were grown in a 13CO2-enriched environment and some leaf material was pretreated with ethanol and detergent in order to remove some cell contents and soluble material. The ethanol- and detergent-treated leaves had less alkyl-C, as seen by solid-state 13C nuclear magnetic spectroscopy (NMR), and a greater proportion of cellulose and hemicellulose than the untreated leaves. Solid-state 13C NMR spectroscopy and scanning electron microscopy (SEM) were used to follow aspects of the C transformations during decomposition. C mineralization was estimated from total CO2 production. The size and activity of the microbial community was greater in limed than in soils without lime, and microbial respiration was less in both soils amended with ethanol- and detergent-treated leaves compared to soils amended with untreated leaves. In both limed and unlimed soils, amendment with untreated leaves led to additional CO2 production within 7 d of addition, whereas amendment with treated leaves led to a smaller increase in CO2 production. The flush of CO2 production was attributed to decomposition of the more accessible and soluble plant components that, in the ethanol- and detergent-treated leaves, had been removed during the ethanol and detergent treatment. The 13C NMR spectra recorded for plant material separated from soil I d after addition of ethanol- and detergent-treated leaves had larger alkyl-C (30 ppm) signals compared with spectra from untreated leaves. This was interpreted as representing an accumulation of residues from decomposition of plant structural components. (C) 2000 Elsevier Science Ltd.

Original languageEnglish
Pages (from-to)301-314
Number of pages14
JournalSoil Biology and Biochemistry
Volume32
Issue number3
DOIs
Publication statusPrint publication - Mar 2000
Externally publishedYes

Keywords

  • C solid-state NMR
  • Lolium perenne
  • Scanning electron microscopy

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