Cellulose acetate membranes functionalized with resveratrol by covalent immobilization for improved osseointegration

A. M. Pandele, P. Neacsu, A. Cimpean, A. I. Staras, F. Miculescu*, A. Iordache, S. I. Voicu, V. K. Thakur, O. D. Toader

*Corresponding author for this work

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Covalent immobilization of resveratrol onto cellulose acetate polymeric membranes used as coating on a Mg-1Ca-0.2Mn-0.6Zr alloy is presented for potential application in the improvement of osseointegration processes. For this purpose, cellulose acetate membrane is hydrolysed in the presence of potassium hydroxide, followed by covalent immobilization of aminopropyl triethoxy silane. Resveratrol was immobilized onto membranes using glutaraldehyde as linker. The newly synthesised functional membranes were thoroughly characterized for their structural characteristics determination employing X-ray photoelectron spectroscopy (XPS), infrared spectroscopy (FT-IR), Raman spectroscopy, thermogravimetric analysis (TGA/DTG) and scanning electron microscopy (SEM) techniques. Subsequently, in vitro cellular tests were performed for evaluating the cytotoxicity biocompatibility of synthesized materials and also the osseointegration potential of obtained derivatised membrane material. It was demonstrated that both polymeric membranes support viability and proliferation of the pre-osteoblastic MC3T3-E1 cells, thus providing a good protection against the potential harmful effects of the compounds released from coated alloys. Furthermore, cellulose acetate membrane functionalized with resveratrol exhibits a significant increase in alkaline phosphatase activity and extracellular matrix mineralization, suggesting its suitability to function as an implant surface coating for guided bone regeneration.

Original languageEnglish
Pages (from-to)2-13
Number of pages12
JournalApplied Surface Science
Volume438
Early online date13 Nov 2017
DOIs
Publication statusPrint publication - 30 Apr 2018
Externally publishedYes

Keywords

  • Cellulose acetate membranes
  • Derivatization
  • Osseointegration
  • Resveratrol

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    Pandele, A. M., Neacsu, P., Cimpean, A., Staras, A. I., Miculescu, F., Iordache, A., Voicu, S. I., Thakur, V. K., & Toader, O. D. (2018). Cellulose acetate membranes functionalized with resveratrol by covalent immobilization for improved osseointegration. Applied Surface Science, 438, 2-13. https://doi.org/10.1016/j.apsusc.2017.11.102