Changes in gene expression during meristem activation processes in Solanum tuberosum with a focus on the regulation of an auxin response factor gene

Odile Faivre-Rampant, Linda Cardle, David Marshall, Roberto Viola, Mark A Taylor

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

A suppression subtractive hybridization approach (SSH) was used to generate a cDNA library enriched in clones representing genes that are up-regulated in the potato tuber apical bud on dormancy release. The sequences of cDNAs representing 385 different genes were determined. This study focuses on the characterization of one of these cDNAs. On the basis of sequence similarity, the cDNA was identified as encoding a member of the auxin response factor family (ARF6). The expression pattern of potato ARF6 was determined by in situ hybridization. In apical tuber buds in the early stages of sprouting, relatively high levels of ARF6-specific transcripts were detected, especially in the peripheral zones of the tunica and corpus of the apical meristems. Expression was also detected in procambial and early vascular tissues, both subtending the meristem and in adjacent leaf primordia. By contrast, in dormant buds no expression of ARF6 could be detected. The expression pattern was also determined during the tuberization process; steady-state expression levels decreased c. 10-fold in the apical region as tuberization proceeded. In non-growing buds, exhibiting correlative inhibition, ARF6-specific transcript levels were relatively low, but rapidly increased when apical dominance was removed by excision of the apical bud. The effects of gibberellin and auxin on axillary bud growth and ARF6 expression are described.
Original languageEnglish
Pages (from-to)613-622
Number of pages10
JournalJournal of Experimental Botany
Volume55
Issue number397
DOIs
Publication statusPrint publication - Mar 2004
Externally publishedYes

Fingerprint

Indoleacetic Acids
Meristem
Solanum tuberosum
meristems
auxins
Complementary DNA
buds
Gene Expression
gene expression
Genes
Gibberellins
genes
Gene Library
In Situ Hybridization
Blood Vessels
tubers
Clone Cells
potatoes
apical dominance
leaf primordia

Keywords

  • Auxin response factor
  • Bud dormancy
  • Meristem activation
  • Solanum tuberosum

Cite this

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title = "Changes in gene expression during meristem activation processes in Solanum tuberosum with a focus on the regulation of an auxin response factor gene",
abstract = "A suppression subtractive hybridization approach (SSH) was used to generate a cDNA library enriched in clones representing genes that are up-regulated in the potato tuber apical bud on dormancy release. The sequences of cDNAs representing 385 different genes were determined. This study focuses on the characterization of one of these cDNAs. On the basis of sequence similarity, the cDNA was identified as encoding a member of the auxin response factor family (ARF6). The expression pattern of potato ARF6 was determined by in situ hybridization. In apical tuber buds in the early stages of sprouting, relatively high levels of ARF6-specific transcripts were detected, especially in the peripheral zones of the tunica and corpus of the apical meristems. Expression was also detected in procambial and early vascular tissues, both subtending the meristem and in adjacent leaf primordia. By contrast, in dormant buds no expression of ARF6 could be detected. The expression pattern was also determined during the tuberization process; steady-state expression levels decreased c. 10-fold in the apical region as tuberization proceeded. In non-growing buds, exhibiting correlative inhibition, ARF6-specific transcript levels were relatively low, but rapidly increased when apical dominance was removed by excision of the apical bud. The effects of gibberellin and auxin on axillary bud growth and ARF6 expression are described.",
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Changes in gene expression during meristem activation processes in Solanum tuberosum with a focus on the regulation of an auxin response factor gene. / Faivre-Rampant, Odile; Cardle, Linda; Marshall, David; Viola, Roberto; Taylor, Mark A.

In: Journal of Experimental Botany, Vol. 55, No. 397, 03.2004, p. 613-622.

Research output: Contribution to journalArticle

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