Characterisation and functional analysis of two barley caleosins expressed during barley caryopsis development

Hui Liu, Peter Hedley, Linda Cardle, Kathryn M. Wright, Ingo Hein, David Marshall, Robbie Waugh

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Two full-length cDNA sequences homologous to caleosin, a seed-storage oil-body protein from sesame, were identified from a series of barley grain development cDNA libraries and further characterised. The cDNAs, subsequently termed HvClo1 and HvClo2, encode proteins of 34 kDa and 28 kDa, respectively. Real-time RT-PCR indicated that HvClo1 is expressed abundantly during the later stages of embryogenesis and is seed-specific, accumulating in the scutellum of mature embryos. HvClo2 is expressed mainly in the endosperm tissues of the developing grain. We show that HvClo1 and HvClo2 are paralogs that co-segregate on barley chromosome 2HL. Transient expression of HvClo1 in lipid storage and non-storage cells of barley using biolistic particle bombardment indicates that caleosins have different subcellular locations from the structural oil-body protein oleosin, and by inference participate in different sorting pathways. We observe that caleosin sorts via small vesicles, suggesting a likely association with lipid trafficking, membrane expansion and oil-body biogenesis.
Original languageEnglish
Pages (from-to)513-522
Number of pages10
JournalPlanta
Volume221
Issue number4
DOIs
Publication statusPrint publication - Jun 2005
Externally publishedYes

Fingerprint

lipid bodies
Hordeum
barley
body protein
fruits
Seeds
Complementary DNA
Biolistics
Sesamum
oleosin
biolistics
Endosperm
Proteins
Sesamum indicum
seed storage
Membrane Lipids
lipids
Sequence Homology
seed development
sequence homology

Bibliographical note

© Springer-Verlag 2005

Keywords

  • Caleosin
  • Gene expression
  • Gene fusions
  • Hordeum vulgare
  • Oil-body biogenesis
  • Oleosin
  • Seed storage

Cite this

Liu, Hui ; Hedley, Peter ; Cardle, Linda ; Wright, Kathryn M. ; Hein, Ingo ; Marshall, David ; Waugh, Robbie. / Characterisation and functional analysis of two barley caleosins expressed during barley caryopsis development. In: Planta. 2005 ; Vol. 221, No. 4. pp. 513-522.
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abstract = "Two full-length cDNA sequences homologous to caleosin, a seed-storage oil-body protein from sesame, were identified from a series of barley grain development cDNA libraries and further characterised. The cDNAs, subsequently termed HvClo1 and HvClo2, encode proteins of 34 kDa and 28 kDa, respectively. Real-time RT-PCR indicated that HvClo1 is expressed abundantly during the later stages of embryogenesis and is seed-specific, accumulating in the scutellum of mature embryos. HvClo2 is expressed mainly in the endosperm tissues of the developing grain. We show that HvClo1 and HvClo2 are paralogs that co-segregate on barley chromosome 2HL. Transient expression of HvClo1 in lipid storage and non-storage cells of barley using biolistic particle bombardment indicates that caleosins have different subcellular locations from the structural oil-body protein oleosin, and by inference participate in different sorting pathways. We observe that caleosin sorts via small vesicles, suggesting a likely association with lipid trafficking, membrane expansion and oil-body biogenesis.",
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Characterisation and functional analysis of two barley caleosins expressed during barley caryopsis development. / Liu, Hui; Hedley, Peter; Cardle, Linda; Wright, Kathryn M.; Hein, Ingo; Marshall, David; Waugh, Robbie.

In: Planta, Vol. 221, No. 4, 06.2005, p. 513-522.

Research output: Contribution to journalArticle

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