Characterisation of Ramularia collo-cygni laboratory mutants resistant to succinate dehydrogenase inhibitors

MJ Piotrowska, JM Fountaine, RA Ennos, M Kaczmarek, FJ Burnett

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

BACKGROUND: Ramularia collo-cygni (Rcc) is responsible for Ramularia leaf spot (RLS), a foliar disease of barley contributing to serious economic losses. Protection against the disease has been almost exclusively based on fungicide applications, including succinate dehydrogenase inhibitors (SDHIs). In2015, the first field isolates of Rcc with reduced sensitivity to SDHIs were recorded in some European countries. In this study we established baseline sensitivity of Rcc to SDHIs in the United Kingdom and characterised mutations correlating with resistance to SDHIs in UV-generated mutants. RESULTS: Five SDHI-resistant isolateswere generated by UV mutagenesis. In four of these mutants a single amino acid change in a target succinate dehydrogenase (Sdh) protein was associated with decrease in sensitivity to SDHIs. Three of these mutations were stably inherited in the absence of SDHI fungicide, and resistant isolates did not demonstrate a fitness penalty. There were no detectable declines in sensitivity in field populations in the years 2010–2012 in the United Kingdom. CONCLUSIONS: SDHIs remained effective in controlling Rcc in the United Kingdom in the years 2010–2012. However, given that the first isolates of Rcc with reduced sensitivity appeared in other European countries in 2015, robust anti resistance strategies need to be continuously implemented to maintain effective disease control. © 2016 Society of Chemical Industry
Original languageEnglish
Pages (from-to)1187 - 1196
Number of pages10
JournalPest Management Science
Volume73
Early online date16 Nov 2016
DOIs
Publication statusFirst published - 16 Nov 2016

Fingerprint

succinate dehydrogenase (quinone)
Ramularia
Cygnus
mutants
United Kingdom
leaf spot
foliar diseases
mutagenesis
pesticide application
fungicides
disease control
barley
mutation
economics
amino acids

Keywords

  • Fungicide insensitivity
  • Pesticides
  • Plant protection
  • Ramularia leaf spot
  • UV mutagenesis

Cite this

Piotrowska, MJ ; Fountaine, JM ; Ennos, RA ; Kaczmarek, M ; Burnett, FJ. / Characterisation of Ramularia collo-cygni laboratory mutants resistant to succinate dehydrogenase inhibitors. In: Pest Management Science. 2016 ; Vol. 73. pp. 1187 - 1196.
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abstract = "BACKGROUND: Ramularia collo-cygni (Rcc) is responsible for Ramularia leaf spot (RLS), a foliar disease of barley contributing to serious economic losses. Protection against the disease has been almost exclusively based on fungicide applications, including succinate dehydrogenase inhibitors (SDHIs). In2015, the first field isolates of Rcc with reduced sensitivity to SDHIs were recorded in some European countries. In this study we established baseline sensitivity of Rcc to SDHIs in the United Kingdom and characterised mutations correlating with resistance to SDHIs in UV-generated mutants. RESULTS: Five SDHI-resistant isolateswere generated by UV mutagenesis. In four of these mutants a single amino acid change in a target succinate dehydrogenase (Sdh) protein was associated with decrease in sensitivity to SDHIs. Three of these mutations were stably inherited in the absence of SDHI fungicide, and resistant isolates did not demonstrate a fitness penalty. There were no detectable declines in sensitivity in field populations in the years 2010–2012 in the United Kingdom. CONCLUSIONS: SDHIs remained effective in controlling Rcc in the United Kingdom in the years 2010–2012. However, given that the first isolates of Rcc with reduced sensitivity appeared in other European countries in 2015, robust anti resistance strategies need to be continuously implemented to maintain effective disease control. {\circledC} 2016 Society of Chemical Industry",
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Characterisation of Ramularia collo-cygni laboratory mutants resistant to succinate dehydrogenase inhibitors. / Piotrowska, MJ; Fountaine, JM; Ennos, RA; Kaczmarek, M; Burnett, FJ.

In: Pest Management Science, Vol. 73, 16.11.2016, p. 1187 - 1196.

Research output: Contribution to journalArticle

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N2 - BACKGROUND: Ramularia collo-cygni (Rcc) is responsible for Ramularia leaf spot (RLS), a foliar disease of barley contributing to serious economic losses. Protection against the disease has been almost exclusively based on fungicide applications, including succinate dehydrogenase inhibitors (SDHIs). In2015, the first field isolates of Rcc with reduced sensitivity to SDHIs were recorded in some European countries. In this study we established baseline sensitivity of Rcc to SDHIs in the United Kingdom and characterised mutations correlating with resistance to SDHIs in UV-generated mutants. RESULTS: Five SDHI-resistant isolateswere generated by UV mutagenesis. In four of these mutants a single amino acid change in a target succinate dehydrogenase (Sdh) protein was associated with decrease in sensitivity to SDHIs. Three of these mutations were stably inherited in the absence of SDHI fungicide, and resistant isolates did not demonstrate a fitness penalty. There were no detectable declines in sensitivity in field populations in the years 2010–2012 in the United Kingdom. CONCLUSIONS: SDHIs remained effective in controlling Rcc in the United Kingdom in the years 2010–2012. However, given that the first isolates of Rcc with reduced sensitivity appeared in other European countries in 2015, robust anti resistance strategies need to be continuously implemented to maintain effective disease control. © 2016 Society of Chemical Industry

AB - BACKGROUND: Ramularia collo-cygni (Rcc) is responsible for Ramularia leaf spot (RLS), a foliar disease of barley contributing to serious economic losses. Protection against the disease has been almost exclusively based on fungicide applications, including succinate dehydrogenase inhibitors (SDHIs). In2015, the first field isolates of Rcc with reduced sensitivity to SDHIs were recorded in some European countries. In this study we established baseline sensitivity of Rcc to SDHIs in the United Kingdom and characterised mutations correlating with resistance to SDHIs in UV-generated mutants. RESULTS: Five SDHI-resistant isolateswere generated by UV mutagenesis. In four of these mutants a single amino acid change in a target succinate dehydrogenase (Sdh) protein was associated with decrease in sensitivity to SDHIs. Three of these mutations were stably inherited in the absence of SDHI fungicide, and resistant isolates did not demonstrate a fitness penalty. There were no detectable declines in sensitivity in field populations in the years 2010–2012 in the United Kingdom. CONCLUSIONS: SDHIs remained effective in controlling Rcc in the United Kingdom in the years 2010–2012. However, given that the first isolates of Rcc with reduced sensitivity appeared in other European countries in 2015, robust anti resistance strategies need to be continuously implemented to maintain effective disease control. © 2016 Society of Chemical Industry

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