Characterisation of the ruminal fermentation and microbiome in lambs supplemented with hydrolysable and condensed tannins

S Salami, B Valenti, M Bella, G Luciano, M O'Grady, J Kerry, E Jones, A Priolo, CJ Newbold

Research output: Contribution to journalArticleResearchpeer-review

1 Downloads (Pure)

Abstract

This study characterised the response of ruminal fermentation and the rumen microbiome in lambs fed commercial vegetal sources of hydrolysable tannins (HT) and condensed tannins (CT). Forty-four lambs (19.56 ± 2.06 kg) were randomly assigned to either a concentrate diet (CON, n = 8) or CON supplemented with 4% of two HT [chestnut (Castanea sativa, HT-c) and tara (Caesalpinia spinosa, HT-t)] and CT [mimosa (Acacia negra, CT-m) and gambier (Uncaria gambir, CT-g)] extracts (all, n = 9) for 75 days pre-slaughter. Tannin supplementation did not influence ruminal fermentation traits. Quantitative PCR demonstrated that tannins did not affect the absolute abundance of ruminal bacteria or fungi. However, CT-m (-12.8%) and CT-g (-11.5%) significantly reduced the abundance of methanogens while HT-t (-20.7%) and CT-g (-20.8%) inhibited protozoal abundance. Ribosomal amplicon sequencing revealed that tannins caused changes in the phylogenetic structure of the bacterial and methanogen communities. Tannins inhibited the fibrolytic bacterium, Fibrobacter and tended to suppress the methanogen genus, Methanosphaera. Results demonstrated that both HT and CT sources could impact the ruminal microbiome when supplemented at 4% inclusion level. HT-t, CT-m and CT-g extracts displayed specific antimicrobial activity against methanogens and protozoa without compromising ruminal fermentation in a long-term feeding trial.
Original languageEnglish
Pages (from-to)1 - 9
Number of pages9
JournalFEMS Microbiology Ecology
Volume94
Issue number5
Early online date10 Apr 2018
DOIs
Publication statusFirst published - 10 Apr 2018

Fingerprint

proanthocyanidins
rumen fermentation
tannins
lambs
methanogens
Fibrobacter
microbiome
rumen fungi
Caesalpinia
Mimosa
rumen bacteria
Castanea sativa
extracts
Acacia
Castanea
Protozoa
rumen
slaughter
quantitative polymerase chain reaction
concentrates

Keywords

  • Bacteria
  • Metagenomics
  • Methanogens
  • Microbiome
  • Ruminal fermentation
  • Tannins

Cite this

Salami, S ; Valenti, B ; Bella, M ; Luciano, G ; O'Grady, M ; Kerry, J ; Jones, E ; Priolo, A ; Newbold, CJ. / Characterisation of the ruminal fermentation and microbiome in lambs supplemented with hydrolysable and condensed tannins. In: FEMS Microbiology Ecology. 2018 ; Vol. 94, No. 5. pp. 1 - 9.
@article{7018de49a0694767a1138fd5ef3deda4,
title = "Characterisation of the ruminal fermentation and microbiome in lambs supplemented with hydrolysable and condensed tannins",
abstract = "This study characterised the response of ruminal fermentation and the rumen microbiome in lambs fed commercial vegetal sources of hydrolysable tannins (HT) and condensed tannins (CT). Forty-four lambs (19.56 ± 2.06 kg) were randomly assigned to either a concentrate diet (CON, n = 8) or CON supplemented with 4{\%} of two HT [chestnut (Castanea sativa, HT-c) and tara (Caesalpinia spinosa, HT-t)] and CT [mimosa (Acacia negra, CT-m) and gambier (Uncaria gambir, CT-g)] extracts (all, n = 9) for 75 days pre-slaughter. Tannin supplementation did not influence ruminal fermentation traits. Quantitative PCR demonstrated that tannins did not affect the absolute abundance of ruminal bacteria or fungi. However, CT-m (-12.8{\%}) and CT-g (-11.5{\%}) significantly reduced the abundance of methanogens while HT-t (-20.7{\%}) and CT-g (-20.8{\%}) inhibited protozoal abundance. Ribosomal amplicon sequencing revealed that tannins caused changes in the phylogenetic structure of the bacterial and methanogen communities. Tannins inhibited the fibrolytic bacterium, Fibrobacter and tended to suppress the methanogen genus, Methanosphaera. Results demonstrated that both HT and CT sources could impact the ruminal microbiome when supplemented at 4{\%} inclusion level. HT-t, CT-m and CT-g extracts displayed specific antimicrobial activity against methanogens and protozoa without compromising ruminal fermentation in a long-term feeding trial.",
keywords = "Bacteria, Metagenomics, Methanogens, Microbiome, Ruminal fermentation, Tannins",
author = "S Salami and B Valenti and M Bella and G Luciano and M O'Grady and J Kerry and E Jones and A Priolo and CJ Newbold",
year = "2018",
month = "4",
day = "10",
doi = "10.1093/femsec/fiy061",
language = "English",
volume = "94",
pages = "1 -- 9",
journal = "FEMS Microbiology Ecology",
issn = "0168-6496",
publisher = "Oxford University Press",
number = "5",

}

Characterisation of the ruminal fermentation and microbiome in lambs supplemented with hydrolysable and condensed tannins. / Salami, S; Valenti, B; Bella, M; Luciano, G; O'Grady, M; Kerry, J; Jones, E; Priolo, A; Newbold, CJ.

In: FEMS Microbiology Ecology, Vol. 94, No. 5, 10.04.2018, p. 1 - 9.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Characterisation of the ruminal fermentation and microbiome in lambs supplemented with hydrolysable and condensed tannins

AU - Salami, S

AU - Valenti, B

AU - Bella, M

AU - Luciano, G

AU - O'Grady, M

AU - Kerry, J

AU - Jones, E

AU - Priolo, A

AU - Newbold, CJ

PY - 2018/4/10

Y1 - 2018/4/10

N2 - This study characterised the response of ruminal fermentation and the rumen microbiome in lambs fed commercial vegetal sources of hydrolysable tannins (HT) and condensed tannins (CT). Forty-four lambs (19.56 ± 2.06 kg) were randomly assigned to either a concentrate diet (CON, n = 8) or CON supplemented with 4% of two HT [chestnut (Castanea sativa, HT-c) and tara (Caesalpinia spinosa, HT-t)] and CT [mimosa (Acacia negra, CT-m) and gambier (Uncaria gambir, CT-g)] extracts (all, n = 9) for 75 days pre-slaughter. Tannin supplementation did not influence ruminal fermentation traits. Quantitative PCR demonstrated that tannins did not affect the absolute abundance of ruminal bacteria or fungi. However, CT-m (-12.8%) and CT-g (-11.5%) significantly reduced the abundance of methanogens while HT-t (-20.7%) and CT-g (-20.8%) inhibited protozoal abundance. Ribosomal amplicon sequencing revealed that tannins caused changes in the phylogenetic structure of the bacterial and methanogen communities. Tannins inhibited the fibrolytic bacterium, Fibrobacter and tended to suppress the methanogen genus, Methanosphaera. Results demonstrated that both HT and CT sources could impact the ruminal microbiome when supplemented at 4% inclusion level. HT-t, CT-m and CT-g extracts displayed specific antimicrobial activity against methanogens and protozoa without compromising ruminal fermentation in a long-term feeding trial.

AB - This study characterised the response of ruminal fermentation and the rumen microbiome in lambs fed commercial vegetal sources of hydrolysable tannins (HT) and condensed tannins (CT). Forty-four lambs (19.56 ± 2.06 kg) were randomly assigned to either a concentrate diet (CON, n = 8) or CON supplemented with 4% of two HT [chestnut (Castanea sativa, HT-c) and tara (Caesalpinia spinosa, HT-t)] and CT [mimosa (Acacia negra, CT-m) and gambier (Uncaria gambir, CT-g)] extracts (all, n = 9) for 75 days pre-slaughter. Tannin supplementation did not influence ruminal fermentation traits. Quantitative PCR demonstrated that tannins did not affect the absolute abundance of ruminal bacteria or fungi. However, CT-m (-12.8%) and CT-g (-11.5%) significantly reduced the abundance of methanogens while HT-t (-20.7%) and CT-g (-20.8%) inhibited protozoal abundance. Ribosomal amplicon sequencing revealed that tannins caused changes in the phylogenetic structure of the bacterial and methanogen communities. Tannins inhibited the fibrolytic bacterium, Fibrobacter and tended to suppress the methanogen genus, Methanosphaera. Results demonstrated that both HT and CT sources could impact the ruminal microbiome when supplemented at 4% inclusion level. HT-t, CT-m and CT-g extracts displayed specific antimicrobial activity against methanogens and protozoa without compromising ruminal fermentation in a long-term feeding trial.

KW - Bacteria

KW - Metagenomics

KW - Methanogens

KW - Microbiome

KW - Ruminal fermentation

KW - Tannins

UR - http://www.mendeley.com/research/characterization-ruminal-fermentation-microbiome-lambs-supplemented-hydrolysable-condensed-tannins

U2 - 10.1093/femsec/fiy061

DO - 10.1093/femsec/fiy061

M3 - Article

VL - 94

SP - 1

EP - 9

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

SN - 0168-6496

IS - 5

ER -