Co-expression analysis of dorsal root ganglia from tail amputated pigs at different ages reveals long-term transcriptional signatures associated with wound healing and inflammation, and neuropathic pain

DA Sandercock*, J Coe, M Barnett, T Freeman, P Di Giminiani, S Edwards

*Corresponding author for this work

Research output: Contribution to conferenceAbstract

Abstract

Concerns exist that docking and biting injuries may be a cause of long term pain in the remaining tail stump during the pig’s lifetime. The potential for long-term pain has been linked to sustained cellular and molecular changes in peripheral sensory neuronal activity. The aim of this study was to conduct a transcriptome analysis of caudal dorsal root ganglia (DRG) gene expression profiles from pigs subjected to tail amputation, in particular examining genes known to be associated with inflammation and neuropathic pain. Microarray analysis was performed on caudal DRG from sham (control) and tail amputated pigs 1, 8 and 16 weeks after tail treatment at either 3 days (neonate) or 63 days (juvenile). Tail amputation injury induced highly significant gene expression changes (both up and down) compared to sham-treated intact controls at both ages (518-2,794 genes, FDR < 0.05) that were still evident 16 weeks after tail amputation. Network correlation analysis using the Markov clustering (MCL) algorithm to define expression modules revealed two highly correlated (PCT r2 ≥0.75), interrelated transcript expression clusters related to (A) neuronal function (759 genes) and (B) wound healing (273 genes). In cluster A, gene ontology (GO) and pathway enrichment analysis identified genes with significant GO terms for voltage- and ligand-gated ion channel activity linked to regulation of membrane potentials, neurotransmitter levels and synaptic signalling. In cluster B significant gene expression was associated with receptor binding, protein transcription activity and regulation, linked to processes such as response to wounding, regulation of response to wounding, inflammatory response and activation of immune response. Cross-reference against an integrated database of known genes involved in the regulation of inflammatory and neuropathic pain revealed 124 and 61 pain–associated genes in clusters A and B, respectively. Key functional families of ion channels and receptors were significantly down-regulated in cluster A, in particular voltage-gated potassium channels and GABA receptors which are linked to increased neuronal excitability. Up-regulated functional gene families in cluster B were mostly linked to inflammation, macrophage activity, neurohormone and opioid peptide activity. DRG gene expression profiles appear to be linked to sustained tissue inflammation and remodelling (ca. 4 months) and pain perception modulation consistent with adaptive, compensatory responses to injury induced increases in peripheral sensory neuron excitability in the injured tail stump. Tail amputation causes acute and sustained changes in peripheral somatosensory nerve function involving inflammatory and neuropathic pain pathways which have implications for pig welfare.
Original languageEnglish
Publication statusFirst published - 28 Jun 2018
EventUFAW International Animal Welfare Science Symposium 2018: Recent advances in animal welfare science VI - Centre for Life, Newcastle upon Tyne, United Kingdom
Duration: 28 Jun 2018 → …
https://www.ufaw.org.uk/ufaw-events/recent-advances-in-animal-welfare-science-vi

Conference

ConferenceUFAW International Animal Welfare Science Symposium 2018
CountryUnited Kingdom
CityNewcastle upon Tyne
Period28/06/18 → …
Internet address

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Spinal Ganglia
Neuralgia
Wound Healing
Tail
Swine
Inflammation
Amputation
Genes
Gene Ontology
Transcriptome
Neurotransmitter Agents
Wounds and Injuries
Ligand-Gated Ion Channels
Voltage-Gated Potassium Channels
Gene Expression
Pain
Pain Perception
GABA Receptors
Opioid Peptides
Gene Expression Profiling

Cite this

Sandercock, DA., Coe, J., Barnett, M., Freeman, T., Di Giminiani, P., & Edwards, S. (2018). Co-expression analysis of dorsal root ganglia from tail amputated pigs at different ages reveals long-term transcriptional signatures associated with wound healing and inflammation, and neuropathic pain. Abstract from UFAW International Animal Welfare Science Symposium 2018, Newcastle upon Tyne, United Kingdom.
Sandercock, DA ; Coe, J ; Barnett, M ; Freeman, T ; Di Giminiani, P ; Edwards, S. / Co-expression analysis of dorsal root ganglia from tail amputated pigs at different ages reveals long-term transcriptional signatures associated with wound healing and inflammation, and neuropathic pain. Abstract from UFAW International Animal Welfare Science Symposium 2018, Newcastle upon Tyne, United Kingdom.
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title = "Co-expression analysis of dorsal root ganglia from tail amputated pigs at different ages reveals long-term transcriptional signatures associated with wound healing and inflammation, and neuropathic pain",
abstract = "Concerns exist that docking and biting injuries may be a cause of long term pain in the remaining tail stump during the pig’s lifetime. The potential for long-term pain has been linked to sustained cellular and molecular changes in peripheral sensory neuronal activity. The aim of this study was to conduct a transcriptome analysis of caudal dorsal root ganglia (DRG) gene expression profiles from pigs subjected to tail amputation, in particular examining genes known to be associated with inflammation and neuropathic pain. Microarray analysis was performed on caudal DRG from sham (control) and tail amputated pigs 1, 8 and 16 weeks after tail treatment at either 3 days (neonate) or 63 days (juvenile). Tail amputation injury induced highly significant gene expression changes (both up and down) compared to sham-treated intact controls at both ages (518-2,794 genes, FDR < 0.05) that were still evident 16 weeks after tail amputation. Network correlation analysis using the Markov clustering (MCL) algorithm to define expression modules revealed two highly correlated (PCT r2 ≥0.75), interrelated transcript expression clusters related to (A) neuronal function (759 genes) and (B) wound healing (273 genes). In cluster A, gene ontology (GO) and pathway enrichment analysis identified genes with significant GO terms for voltage- and ligand-gated ion channel activity linked to regulation of membrane potentials, neurotransmitter levels and synaptic signalling. In cluster B significant gene expression was associated with receptor binding, protein transcription activity and regulation, linked to processes such as response to wounding, regulation of response to wounding, inflammatory response and activation of immune response. Cross-reference against an integrated database of known genes involved in the regulation of inflammatory and neuropathic pain revealed 124 and 61 pain–associated genes in clusters A and B, respectively. Key functional families of ion channels and receptors were significantly down-regulated in cluster A, in particular voltage-gated potassium channels and GABA receptors which are linked to increased neuronal excitability. Up-regulated functional gene families in cluster B were mostly linked to inflammation, macrophage activity, neurohormone and opioid peptide activity. DRG gene expression profiles appear to be linked to sustained tissue inflammation and remodelling (ca. 4 months) and pain perception modulation consistent with adaptive, compensatory responses to injury induced increases in peripheral sensory neuron excitability in the injured tail stump. Tail amputation causes acute and sustained changes in peripheral somatosensory nerve function involving inflammatory and neuropathic pain pathways which have implications for pig welfare.",
author = "DA Sandercock and J Coe and M Barnett and T Freeman and {Di Giminiani}, P and S Edwards",
year = "2018",
month = "6",
day = "28",
language = "English",
note = "UFAW International Animal Welfare Science Symposium 2018 : Recent advances in animal welfare science VI ; Conference date: 28-06-2018",
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}

Sandercock, DA, Coe, J, Barnett, M, Freeman, T, Di Giminiani, P & Edwards, S 2018, 'Co-expression analysis of dorsal root ganglia from tail amputated pigs at different ages reveals long-term transcriptional signatures associated with wound healing and inflammation, and neuropathic pain', UFAW International Animal Welfare Science Symposium 2018, Newcastle upon Tyne, United Kingdom, 28/06/18.

Co-expression analysis of dorsal root ganglia from tail amputated pigs at different ages reveals long-term transcriptional signatures associated with wound healing and inflammation, and neuropathic pain. / Sandercock, DA; Coe, J; Barnett, M; Freeman, T; Di Giminiani, P; Edwards, S.

2018. Abstract from UFAW International Animal Welfare Science Symposium 2018, Newcastle upon Tyne, United Kingdom.

Research output: Contribution to conferenceAbstract

TY - CONF

T1 - Co-expression analysis of dorsal root ganglia from tail amputated pigs at different ages reveals long-term transcriptional signatures associated with wound healing and inflammation, and neuropathic pain

AU - Sandercock, DA

AU - Coe, J

AU - Barnett, M

AU - Freeman, T

AU - Di Giminiani, P

AU - Edwards, S

PY - 2018/6/28

Y1 - 2018/6/28

N2 - Concerns exist that docking and biting injuries may be a cause of long term pain in the remaining tail stump during the pig’s lifetime. The potential for long-term pain has been linked to sustained cellular and molecular changes in peripheral sensory neuronal activity. The aim of this study was to conduct a transcriptome analysis of caudal dorsal root ganglia (DRG) gene expression profiles from pigs subjected to tail amputation, in particular examining genes known to be associated with inflammation and neuropathic pain. Microarray analysis was performed on caudal DRG from sham (control) and tail amputated pigs 1, 8 and 16 weeks after tail treatment at either 3 days (neonate) or 63 days (juvenile). Tail amputation injury induced highly significant gene expression changes (both up and down) compared to sham-treated intact controls at both ages (518-2,794 genes, FDR < 0.05) that were still evident 16 weeks after tail amputation. Network correlation analysis using the Markov clustering (MCL) algorithm to define expression modules revealed two highly correlated (PCT r2 ≥0.75), interrelated transcript expression clusters related to (A) neuronal function (759 genes) and (B) wound healing (273 genes). In cluster A, gene ontology (GO) and pathway enrichment analysis identified genes with significant GO terms for voltage- and ligand-gated ion channel activity linked to regulation of membrane potentials, neurotransmitter levels and synaptic signalling. In cluster B significant gene expression was associated with receptor binding, protein transcription activity and regulation, linked to processes such as response to wounding, regulation of response to wounding, inflammatory response and activation of immune response. Cross-reference against an integrated database of known genes involved in the regulation of inflammatory and neuropathic pain revealed 124 and 61 pain–associated genes in clusters A and B, respectively. Key functional families of ion channels and receptors were significantly down-regulated in cluster A, in particular voltage-gated potassium channels and GABA receptors which are linked to increased neuronal excitability. Up-regulated functional gene families in cluster B were mostly linked to inflammation, macrophage activity, neurohormone and opioid peptide activity. DRG gene expression profiles appear to be linked to sustained tissue inflammation and remodelling (ca. 4 months) and pain perception modulation consistent with adaptive, compensatory responses to injury induced increases in peripheral sensory neuron excitability in the injured tail stump. Tail amputation causes acute and sustained changes in peripheral somatosensory nerve function involving inflammatory and neuropathic pain pathways which have implications for pig welfare.

AB - Concerns exist that docking and biting injuries may be a cause of long term pain in the remaining tail stump during the pig’s lifetime. The potential for long-term pain has been linked to sustained cellular and molecular changes in peripheral sensory neuronal activity. The aim of this study was to conduct a transcriptome analysis of caudal dorsal root ganglia (DRG) gene expression profiles from pigs subjected to tail amputation, in particular examining genes known to be associated with inflammation and neuropathic pain. Microarray analysis was performed on caudal DRG from sham (control) and tail amputated pigs 1, 8 and 16 weeks after tail treatment at either 3 days (neonate) or 63 days (juvenile). Tail amputation injury induced highly significant gene expression changes (both up and down) compared to sham-treated intact controls at both ages (518-2,794 genes, FDR < 0.05) that were still evident 16 weeks after tail amputation. Network correlation analysis using the Markov clustering (MCL) algorithm to define expression modules revealed two highly correlated (PCT r2 ≥0.75), interrelated transcript expression clusters related to (A) neuronal function (759 genes) and (B) wound healing (273 genes). In cluster A, gene ontology (GO) and pathway enrichment analysis identified genes with significant GO terms for voltage- and ligand-gated ion channel activity linked to regulation of membrane potentials, neurotransmitter levels and synaptic signalling. In cluster B significant gene expression was associated with receptor binding, protein transcription activity and regulation, linked to processes such as response to wounding, regulation of response to wounding, inflammatory response and activation of immune response. Cross-reference against an integrated database of known genes involved in the regulation of inflammatory and neuropathic pain revealed 124 and 61 pain–associated genes in clusters A and B, respectively. Key functional families of ion channels and receptors were significantly down-regulated in cluster A, in particular voltage-gated potassium channels and GABA receptors which are linked to increased neuronal excitability. Up-regulated functional gene families in cluster B were mostly linked to inflammation, macrophage activity, neurohormone and opioid peptide activity. DRG gene expression profiles appear to be linked to sustained tissue inflammation and remodelling (ca. 4 months) and pain perception modulation consistent with adaptive, compensatory responses to injury induced increases in peripheral sensory neuron excitability in the injured tail stump. Tail amputation causes acute and sustained changes in peripheral somatosensory nerve function involving inflammatory and neuropathic pain pathways which have implications for pig welfare.

M3 - Abstract

ER -

Sandercock DA, Coe J, Barnett M, Freeman T, Di Giminiani P, Edwards S. Co-expression analysis of dorsal root ganglia from tail amputated pigs at different ages reveals long-term transcriptional signatures associated with wound healing and inflammation, and neuropathic pain. 2018. Abstract from UFAW International Animal Welfare Science Symposium 2018, Newcastle upon Tyne, United Kingdom.