Co-fermentation of residual algal biomass and glucose under the influence of Fe₃O₄ nanoparticles to enhance biohydrogen production under dark mode

The present study reports Fe₃O₄ nanoparticles (Fe₃O₄ NPs) induced enhanced hydrogen production via co-fermentation of glucose and residual algal biomass (cyanobacteria Lyngbya limnetica). A significant enhancement of dark fermentative H₂ production has been noticed under the influence of co-fermentation of glucose and residual algal biomass using Fe₃O₄ NPs as catalyst. Further, using the optimized ratio of glucose to residual algal biomass (10:4), ∼ 37.14 % higher cumulative H₂ has been recorded in presence of 7.5 mg/L Fe₃O₄ NPs as compared to control at 37 °C. In addition, under the optimum conditions [glucose to residual algal biomass ratio (10:4)] presence of 7.5 mg/L Fe₃O₄ NPs produces ∼ 937 mL/L cumulative H₂ in 168 h at pH 7.5 and at temperature 40 °C. Clostridum butyrium, employed for the dark fermentation yielded ∼ 7.7 g/L dry biomass in 168 h whereas acetate (9.0 g/L) and butyrate (6.2 g/L) have been recorded as the dominating metabolites.