Comparing microbiotas in the upper aerodigestive and lower respiratory tracts of lambs

L Glendinning*, D Collie, S Wright, KMD Rutherford, G McLachlan

*Corresponding author for this work

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background Recently, the importance of the lung microbiota during health and disease has been examined in humans and in small animal models. Whilst sheep have been proposed as an appropriate large animal model for studying the pathophysiology of a number of important human respiratory diseases, it is clearly important to continually define the limits of agreement between these systems as new concepts emerge. In humans, it has recently been established that the lung microbiota is seeded by microbes from the oral cavity. We sought to determine whether the same was true in sheep. Results We took lung fluid and upper aerodigestive tract (oropharyngeal) swab samples from 40 lambs (7 weeks old). DNA extraction was performed, and the V2-V3 region of the 16S rRNA gene was amplified by PCR then sequenced via Illumina Miseq. Oropharyngeal swabs were either dominated by bacteria commonly associated with the rumen or by bacteria commonly associated with the upper aerodigestive tract. Lung microbiota samples did not resemble either the upper aerodigestive tract samples or reagent-only controls. Some rumen-associated bacteria were found in lung fluids, indicating that inhalation of ruminal bacteria does occur. We also identified several bacteria which were significantly more abundant in lung fluids than in the upper aerodigestive tract swabs, the most predominant of which was classified as Staphylococcus equorum. Conclusions In contrast to humans, we found that the lung microbiota of lambs is dissimilar to that of the upper aerodigestive tract, and we suggest that this may be related to physiological and anatomical differences between sheep and humans. Understanding the comparative physiology and anatomy underlying differences in lung microbiota between species will provide a foundation upon which to interpret changes associated with disease and/or environment.
Original languageEnglish
JournalMicrobiome
Volume5
Issue number145
Early online date27 Oct 2017
DOIs
Publication statusFirst published - 27 Oct 2017

Fingerprint

Microbiota
Respiratory System
Lung
Bacteria
Sheep
Rumen
Animal Models
Comparative Physiology
Comparative Anatomy
Staphylococcus
rRNA Genes
Inhalation
Mouth
Polymerase Chain Reaction
DNA
Health

Bibliographical note

1023403

Keywords

  • 16S
  • Lambs
  • Lung
  • Microbiota
  • Oropharynx
  • Rumen
  • Sheep

Cite this

Glendinning, L ; Collie, D ; Wright, S ; Rutherford, KMD ; McLachlan, G. / Comparing microbiotas in the upper aerodigestive and lower respiratory tracts of lambs. In: Microbiome. 2017 ; Vol. 5, No. 145.
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Comparing microbiotas in the upper aerodigestive and lower respiratory tracts of lambs. / Glendinning, L; Collie, D; Wright, S; Rutherford, KMD; McLachlan, G.

In: Microbiome, Vol. 5, No. 145, 27.10.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Comparing microbiotas in the upper aerodigestive and lower respiratory tracts of lambs

AU - Glendinning, L

AU - Collie, D

AU - Wright, S

AU - Rutherford, KMD

AU - McLachlan, G

N1 - 1023403

PY - 2017/10/27

Y1 - 2017/10/27

N2 - Background Recently, the importance of the lung microbiota during health and disease has been examined in humans and in small animal models. Whilst sheep have been proposed as an appropriate large animal model for studying the pathophysiology of a number of important human respiratory diseases, it is clearly important to continually define the limits of agreement between these systems as new concepts emerge. In humans, it has recently been established that the lung microbiota is seeded by microbes from the oral cavity. We sought to determine whether the same was true in sheep. Results We took lung fluid and upper aerodigestive tract (oropharyngeal) swab samples from 40 lambs (7 weeks old). DNA extraction was performed, and the V2-V3 region of the 16S rRNA gene was amplified by PCR then sequenced via Illumina Miseq. Oropharyngeal swabs were either dominated by bacteria commonly associated with the rumen or by bacteria commonly associated with the upper aerodigestive tract. Lung microbiota samples did not resemble either the upper aerodigestive tract samples or reagent-only controls. Some rumen-associated bacteria were found in lung fluids, indicating that inhalation of ruminal bacteria does occur. We also identified several bacteria which were significantly more abundant in lung fluids than in the upper aerodigestive tract swabs, the most predominant of which was classified as Staphylococcus equorum. Conclusions In contrast to humans, we found that the lung microbiota of lambs is dissimilar to that of the upper aerodigestive tract, and we suggest that this may be related to physiological and anatomical differences between sheep and humans. Understanding the comparative physiology and anatomy underlying differences in lung microbiota between species will provide a foundation upon which to interpret changes associated with disease and/or environment.

AB - Background Recently, the importance of the lung microbiota during health and disease has been examined in humans and in small animal models. Whilst sheep have been proposed as an appropriate large animal model for studying the pathophysiology of a number of important human respiratory diseases, it is clearly important to continually define the limits of agreement between these systems as new concepts emerge. In humans, it has recently been established that the lung microbiota is seeded by microbes from the oral cavity. We sought to determine whether the same was true in sheep. Results We took lung fluid and upper aerodigestive tract (oropharyngeal) swab samples from 40 lambs (7 weeks old). DNA extraction was performed, and the V2-V3 region of the 16S rRNA gene was amplified by PCR then sequenced via Illumina Miseq. Oropharyngeal swabs were either dominated by bacteria commonly associated with the rumen or by bacteria commonly associated with the upper aerodigestive tract. Lung microbiota samples did not resemble either the upper aerodigestive tract samples or reagent-only controls. Some rumen-associated bacteria were found in lung fluids, indicating that inhalation of ruminal bacteria does occur. We also identified several bacteria which were significantly more abundant in lung fluids than in the upper aerodigestive tract swabs, the most predominant of which was classified as Staphylococcus equorum. Conclusions In contrast to humans, we found that the lung microbiota of lambs is dissimilar to that of the upper aerodigestive tract, and we suggest that this may be related to physiological and anatomical differences between sheep and humans. Understanding the comparative physiology and anatomy underlying differences in lung microbiota between species will provide a foundation upon which to interpret changes associated with disease and/or environment.

KW - 16S

KW - Lambs

KW - Lung

KW - Microbiota

KW - Oropharynx

KW - Rumen

KW - Sheep

U2 - 10.1186/s40168-017-0364-5

DO - 10.1186/s40168-017-0364-5

M3 - Article

VL - 5

JO - Microbiome

JF - Microbiome

SN - 2049-2618

IS - 145

ER -