Constructing plant radiation hybrid panels

Julie Wardrop, John Snape, Wayne Powell, Gordon C Machray

Research output: Contribution to journalArticleResearchpeer-review

36 Citations (Scopus)

Abstract

Radiation hybrid (RH) mapping, a somatic cell genetic technique, has been developed in animal systems as a general approach for the construction of long-range physical maps of chromosomes. This statistical method relies on X-ray induced breakage of chromosomes to determine the physical distance between markers, as well as their order on the chromosome. The method can be applied to single chromosomes or across the whole genome. The generation of plant (barley) radiation hybrids and their culture in vitro is described here. PCR-based marker systems are used to verify hybrid status and to demonstrate genome coverage. RH panels of the type generated can be used for physical mapping, map-based cloning, or sequence contig assembly. RH resources will greatly aid the physical characterisation of crop plants with large genomes.

Original languageEnglish
Pages (from-to)223-8
Number of pages6
JournalPlant Soil
Volume31
Issue number2
Publication statusPrint publication - Jul 2002
Externally publishedYes

Fingerprint

Radiation Hybrid Mapping
Chromosomes
Genome
Genetic Techniques
Chromosome Breakage
Hordeum
Organism Cloning
X-Rays
Polymerase Chain Reaction

Keywords

  • Cells, Cultured
  • Genome, Plant
  • Hordeum/genetics
  • Radiation Hybrid Mapping/methods
  • Reproducibility of Results

Cite this

Wardrop, J., Snape, J., Powell, W., & Machray, G. C. (2002). Constructing plant radiation hybrid panels. Plant Soil, 31(2), 223-8.
Wardrop, Julie ; Snape, John ; Powell, Wayne ; Machray, Gordon C. / Constructing plant radiation hybrid panels. In: Plant Soil. 2002 ; Vol. 31, No. 2. pp. 223-8.
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Wardrop, J, Snape, J, Powell, W & Machray, GC 2002, 'Constructing plant radiation hybrid panels', Plant Soil, vol. 31, no. 2, pp. 223-8.

Constructing plant radiation hybrid panels. / Wardrop, Julie; Snape, John; Powell, Wayne; Machray, Gordon C.

In: Plant Soil, Vol. 31, No. 2, 07.2002, p. 223-8.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Constructing plant radiation hybrid panels

AU - Wardrop, Julie

AU - Snape, John

AU - Powell, Wayne

AU - Machray, Gordon C

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N2 - Radiation hybrid (RH) mapping, a somatic cell genetic technique, has been developed in animal systems as a general approach for the construction of long-range physical maps of chromosomes. This statistical method relies on X-ray induced breakage of chromosomes to determine the physical distance between markers, as well as their order on the chromosome. The method can be applied to single chromosomes or across the whole genome. The generation of plant (barley) radiation hybrids and their culture in vitro is described here. PCR-based marker systems are used to verify hybrid status and to demonstrate genome coverage. RH panels of the type generated can be used for physical mapping, map-based cloning, or sequence contig assembly. RH resources will greatly aid the physical characterisation of crop plants with large genomes.

AB - Radiation hybrid (RH) mapping, a somatic cell genetic technique, has been developed in animal systems as a general approach for the construction of long-range physical maps of chromosomes. This statistical method relies on X-ray induced breakage of chromosomes to determine the physical distance between markers, as well as their order on the chromosome. The method can be applied to single chromosomes or across the whole genome. The generation of plant (barley) radiation hybrids and their culture in vitro is described here. PCR-based marker systems are used to verify hybrid status and to demonstrate genome coverage. RH panels of the type generated can be used for physical mapping, map-based cloning, or sequence contig assembly. RH resources will greatly aid the physical characterisation of crop plants with large genomes.

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Wardrop J, Snape J, Powell W, Machray GC. Constructing plant radiation hybrid panels. Plant Soil. 2002 Jul;31(2):223-8.