Contribution of rumen protozoa to duodenal flow of nitrogen, conjugated linoleic acid and vaccenic acid in steers fed silages differing in their water-soluble carbohydrate content

David R Yáñez-Ruiz, Nigel D Scollan, Roger J Merry, Charles J Newbold

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The present experiment was designed to estimate the quantitative contribution of rumen protozoa to the total N, conjugated linoleic acid (CLA) and vaccenic acid (VA; trans-11-18 : 1) flow to the duodenum of steers fed two silage diets: control silage (CS) and silage high in water-soluble carbohydrates (HS). Protozoal duodenal flows were estimated using a real-time PCR assay to quantify the genes encoding protozoal 18S ribosomal RNA. Denaturing gradient gel electrophoresis was used to confirm that the rumen protozoa populations were similar to the protozoal population flowing to the duodenum. Estimated duodenal flow of protozoal N was 14.2 and 18.2 g/d (P>0.05) for animals fed the CS and HS diets respectively. Protozoal flow thus represented between 12 and 15 % of the total N duodenal flow. In terms of fatty acid flow, protozoa accounted for between 30 and 43 % of the CLA and 40 % of the VA reaching the duodenum. The contribution of protozoa to 16 : 0 and 18 : 0 flows to the duodenum was less than 20 and 10 %, respectively. These results show that the fatty acids within protozoa make up a significant proportion of the CLA and VA reaching the duodenum of ruminants.

Original languageEnglish
Pages (from-to)861-9
Number of pages9
JournalBritish Journal of Nutrition
Volume96
Issue number5
Publication statusPrint publication - Nov 2006
Externally publishedYes

Fingerprint

Conjugated Linoleic Acids
Silage
Rumen
Duodenum
Nitrogen
Carbohydrates
Water
Fatty Acids
18S Ribosomal RNA
Diet
Denaturing Gradient Gel Electrophoresis
Ruminants
Population
Real-Time Polymerase Chain Reaction
11-octadecenoic acid
Genes

Keywords

  • Animals
  • Cattle
  • DNA, Protozoan/analysis
  • Dietary Carbohydrates/administration & dosage
  • Duodenum/physiology
  • Electrophoresis, Agar Gel/methods
  • Eukaryota/chemistry
  • Fatty Acids/analysis
  • Linoleic Acids, Conjugated/pharmacokinetics
  • Male
  • Nitrogen/pharmacokinetics
  • Oleic Acids/pharmacokinetics
  • Rumen/physiology
  • Silage/analysis
  • Solubility

Cite this

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title = "Contribution of rumen protozoa to duodenal flow of nitrogen, conjugated linoleic acid and vaccenic acid in steers fed silages differing in their water-soluble carbohydrate content",
abstract = "The present experiment was designed to estimate the quantitative contribution of rumen protozoa to the total N, conjugated linoleic acid (CLA) and vaccenic acid (VA; trans-11-18 : 1) flow to the duodenum of steers fed two silage diets: control silage (CS) and silage high in water-soluble carbohydrates (HS). Protozoal duodenal flows were estimated using a real-time PCR assay to quantify the genes encoding protozoal 18S ribosomal RNA. Denaturing gradient gel electrophoresis was used to confirm that the rumen protozoa populations were similar to the protozoal population flowing to the duodenum. Estimated duodenal flow of protozoal N was 14.2 and 18.2 g/d (P>0.05) for animals fed the CS and HS diets respectively. Protozoal flow thus represented between 12 and 15 {\%} of the total N duodenal flow. In terms of fatty acid flow, protozoa accounted for between 30 and 43 {\%} of the CLA and 40 {\%} of the VA reaching the duodenum. The contribution of protozoa to 16 : 0 and 18 : 0 flows to the duodenum was less than 20 and 10 {\%}, respectively. These results show that the fatty acids within protozoa make up a significant proportion of the CLA and VA reaching the duodenum of ruminants.",
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Contribution of rumen protozoa to duodenal flow of nitrogen, conjugated linoleic acid and vaccenic acid in steers fed silages differing in their water-soluble carbohydrate content. / Yáñez-Ruiz, David R; Scollan, Nigel D; Merry, Roger J; Newbold, Charles J.

In: British Journal of Nutrition, Vol. 96, No. 5, 11.2006, p. 861-9.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Contribution of rumen protozoa to duodenal flow of nitrogen, conjugated linoleic acid and vaccenic acid in steers fed silages differing in their water-soluble carbohydrate content

AU - Yáñez-Ruiz, David R

AU - Scollan, Nigel D

AU - Merry, Roger J

AU - Newbold, Charles J

PY - 2006/11

Y1 - 2006/11

N2 - The present experiment was designed to estimate the quantitative contribution of rumen protozoa to the total N, conjugated linoleic acid (CLA) and vaccenic acid (VA; trans-11-18 : 1) flow to the duodenum of steers fed two silage diets: control silage (CS) and silage high in water-soluble carbohydrates (HS). Protozoal duodenal flows were estimated using a real-time PCR assay to quantify the genes encoding protozoal 18S ribosomal RNA. Denaturing gradient gel electrophoresis was used to confirm that the rumen protozoa populations were similar to the protozoal population flowing to the duodenum. Estimated duodenal flow of protozoal N was 14.2 and 18.2 g/d (P>0.05) for animals fed the CS and HS diets respectively. Protozoal flow thus represented between 12 and 15 % of the total N duodenal flow. In terms of fatty acid flow, protozoa accounted for between 30 and 43 % of the CLA and 40 % of the VA reaching the duodenum. The contribution of protozoa to 16 : 0 and 18 : 0 flows to the duodenum was less than 20 and 10 %, respectively. These results show that the fatty acids within protozoa make up a significant proportion of the CLA and VA reaching the duodenum of ruminants.

AB - The present experiment was designed to estimate the quantitative contribution of rumen protozoa to the total N, conjugated linoleic acid (CLA) and vaccenic acid (VA; trans-11-18 : 1) flow to the duodenum of steers fed two silage diets: control silage (CS) and silage high in water-soluble carbohydrates (HS). Protozoal duodenal flows were estimated using a real-time PCR assay to quantify the genes encoding protozoal 18S ribosomal RNA. Denaturing gradient gel electrophoresis was used to confirm that the rumen protozoa populations were similar to the protozoal population flowing to the duodenum. Estimated duodenal flow of protozoal N was 14.2 and 18.2 g/d (P>0.05) for animals fed the CS and HS diets respectively. Protozoal flow thus represented between 12 and 15 % of the total N duodenal flow. In terms of fatty acid flow, protozoa accounted for between 30 and 43 % of the CLA and 40 % of the VA reaching the duodenum. The contribution of protozoa to 16 : 0 and 18 : 0 flows to the duodenum was less than 20 and 10 %, respectively. These results show that the fatty acids within protozoa make up a significant proportion of the CLA and VA reaching the duodenum of ruminants.

KW - Animals

KW - Cattle

KW - DNA, Protozoan/analysis

KW - Dietary Carbohydrates/administration & dosage

KW - Duodenum/physiology

KW - Electrophoresis, Agar Gel/methods

KW - Eukaryota/chemistry

KW - Fatty Acids/analysis

KW - Linoleic Acids, Conjugated/pharmacokinetics

KW - Male

KW - Nitrogen/pharmacokinetics

KW - Oleic Acids/pharmacokinetics

KW - Rumen/physiology

KW - Silage/analysis

KW - Solubility

M3 - Article

C2 - 17092374

VL - 96

SP - 861

EP - 869

JO - British Journal of Nutrition

JF - British Journal of Nutrition

SN - 0007-1145

IS - 5

ER -