Cytosporone B as a biological preservative: Purification, fungicidal activity and mechanism of action against Geotrichum citri-aurantii

Chunxiao Yin, Hongxin Liu, Yang Shan, Vijai Kumar Gupta*, Yueming Jiang, Weimin Zhang, Haibo Tan, Liang Gong

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

To prevent citrus decay caused by Geotrichum citri-aurantii, 12 natural products were isolated from two endophytic fungi, in which cytosporone B was shown to have excellent bioactivity for control of G. citri-aurantii with median effect concentration (EC50) of 26.11 μg/mL and minimum inhibitory concentration (MIC) of 105 μg/mL, and also significantly reduced the decay of sugar orange during the in vivo trials. In addition, cytosporone B could alter the morphology of G. citri-aurantii by causing distortion of the mycelia and loss of membrane integrity. Differentially expressed genes (DEGs) between cytosporone B-treated and -untreated samples were revealed by Illumina sequencing, including 3540 unigenes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that most DEGs were related to metabolic production and cell membrane. These findings suggest cytosporone B is a promising biological preservative to control citrus decay and reveal the action mechanism of cytosporone B in relation to the destruction of the fungal cell membrane at both morphological and molecular levels.

Original languageEnglish
Article number125
JournalBiomolecules
Volume9
Issue number4
Early online date29 Mar 2019
DOIs
Publication statusPrint publication - Apr 2019
Externally publishedYes

Keywords

  • Citrus decay
  • Cytosporone B
  • Geotrichum citri-aurantii
  • Mode of action
  • RNA-Seq

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