Detection and pattern of interspecific hybridization between Gliricidia septum and G. maculata in Meso-America revealed by PCR-based assays

I. K. Dawson, A. J. Simons, R. Waugh, W. Powell

Research output: Contribution to journalArticleResearchpeer-review

24 Citations (Scopus)

Abstract

Gliricidia sepium provides a variety of products important for rural communities in tropical countries. Native populations in Meso-America currently form an important source of seed for distribution to farmers, but concerns centre on mechanisms which may lead to their genetic erosion, including anthropogenic dispersal and subsequent introgression from the related species, G. maculata. Populations of Gliricidia were examined genetically using approaches based on the polymerase chain reaction to test for interspecific hybridization and introgression between G. sepium and G. maculata. Analysis involved 13 RAPD and two RFLP-PCR markers which were identified to have species-diagnostic distributions. Data from both approaches corresponded and indicated three locations where multilocus genotypes were consistent with an hybrid origin. Data at one of these sites was consistent with introgression following hybridization. The hybrid origin of populations was supported by the intermediate geographical location of these sites to 'pure' populations of each species. Analysis of maternally inherited organellar DNA, which involved the detection of SSCPs in mitochondrial DNA amplification products, allowed further delineation of genetic structure among Gliricidia populations. Mitochondrial data indicated a high degree of organelle differentiation between sampled locations and identified G. sepium- and G. maculatadiagnostic haplotypes. This data supported the interpretation of genetic structure based on RAPDs and RFLP-PCR. In addition, cytonuclear analysis allowed the directionality of gene transfer during the formation of hybrid populations to be described. Despite evidence for the occurrence of interspecific hybridization and introgression in Gliricidia, important resource populations of G. sepium on the Pacific coast appear to have retained their genetic integrity. Implications in terms of the conservation and utilization of genetic resources within the genus are discussed.
Original languageEnglish
Pages (from-to)89-98
Number of pages10
JournalMolecular Ecology
Volume5
Issue number1
DOIs
Publication statusPrint publication - 1996
Externally publishedYes

Fingerprint

Gliricidia maculata
Gliricidia
interspecific hybridization
introgression
Sepia
Gliricidia sepium
assay
Polymerase Chain Reaction
assays
genetic structure
Population
Genetic Structures
genetic resource
gene transfer
Restriction Fragment Length Polymorphisms
restriction fragment length polymorphism
mitochondrial DNA
polymerase chain reaction
amplification
Random Amplified Polymorphic DNA Technique

Keywords

  • G. maculata
  • Genetic erosion
  • Gliricidia sepium
  • Interspecific gene flow
  • Mitochondrial DNA
  • RAPDs
  • RFLPs
  • SSCPs

Cite this

@article{35e7ebcbafb84f1aaa29d319158443fb,
title = "Detection and pattern of interspecific hybridization between Gliricidia septum and G. maculata in Meso-America revealed by PCR-based assays",
abstract = "Gliricidia sepium provides a variety of products important for rural communities in tropical countries. Native populations in Meso-America currently form an important source of seed for distribution to farmers, but concerns centre on mechanisms which may lead to their genetic erosion, including anthropogenic dispersal and subsequent introgression from the related species, G. maculata. Populations of Gliricidia were examined genetically using approaches based on the polymerase chain reaction to test for interspecific hybridization and introgression between G. sepium and G. maculata. Analysis involved 13 RAPD and two RFLP-PCR markers which were identified to have species-diagnostic distributions. Data from both approaches corresponded and indicated three locations where multilocus genotypes were consistent with an hybrid origin. Data at one of these sites was consistent with introgression following hybridization. The hybrid origin of populations was supported by the intermediate geographical location of these sites to 'pure' populations of each species. Analysis of maternally inherited organellar DNA, which involved the detection of SSCPs in mitochondrial DNA amplification products, allowed further delineation of genetic structure among Gliricidia populations. Mitochondrial data indicated a high degree of organelle differentiation between sampled locations and identified G. sepium- and G. maculatadiagnostic haplotypes. This data supported the interpretation of genetic structure based on RAPDs and RFLP-PCR. In addition, cytonuclear analysis allowed the directionality of gene transfer during the formation of hybrid populations to be described. Despite evidence for the occurrence of interspecific hybridization and introgression in Gliricidia, important resource populations of G. sepium on the Pacific coast appear to have retained their genetic integrity. Implications in terms of the conservation and utilization of genetic resources within the genus are discussed.",
keywords = "G. maculata, Genetic erosion, Gliricidia sepium, Interspecific gene flow, Mitochondrial DNA, RAPDs, RFLPs, SSCPs",
author = "Dawson, {I. K.} and Simons, {A. J.} and R. Waugh and W. Powell",
year = "1996",
doi = "10.1111/j.1365-294X.1996.tb00294.x",
language = "English",
volume = "5",
pages = "89--98",
journal = "Molecular Ecology",
issn = "0962-1083",
publisher = "Wiley Blackwell",
number = "1",

}

Detection and pattern of interspecific hybridization between Gliricidia septum and G. maculata in Meso-America revealed by PCR-based assays. / Dawson, I. K.; Simons, A. J.; Waugh, R.; Powell, W.

In: Molecular Ecology, Vol. 5, No. 1, 1996, p. 89-98.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Detection and pattern of interspecific hybridization between Gliricidia septum and G. maculata in Meso-America revealed by PCR-based assays

AU - Dawson, I. K.

AU - Simons, A. J.

AU - Waugh, R.

AU - Powell, W.

PY - 1996

Y1 - 1996

N2 - Gliricidia sepium provides a variety of products important for rural communities in tropical countries. Native populations in Meso-America currently form an important source of seed for distribution to farmers, but concerns centre on mechanisms which may lead to their genetic erosion, including anthropogenic dispersal and subsequent introgression from the related species, G. maculata. Populations of Gliricidia were examined genetically using approaches based on the polymerase chain reaction to test for interspecific hybridization and introgression between G. sepium and G. maculata. Analysis involved 13 RAPD and two RFLP-PCR markers which were identified to have species-diagnostic distributions. Data from both approaches corresponded and indicated three locations where multilocus genotypes were consistent with an hybrid origin. Data at one of these sites was consistent with introgression following hybridization. The hybrid origin of populations was supported by the intermediate geographical location of these sites to 'pure' populations of each species. Analysis of maternally inherited organellar DNA, which involved the detection of SSCPs in mitochondrial DNA amplification products, allowed further delineation of genetic structure among Gliricidia populations. Mitochondrial data indicated a high degree of organelle differentiation between sampled locations and identified G. sepium- and G. maculatadiagnostic haplotypes. This data supported the interpretation of genetic structure based on RAPDs and RFLP-PCR. In addition, cytonuclear analysis allowed the directionality of gene transfer during the formation of hybrid populations to be described. Despite evidence for the occurrence of interspecific hybridization and introgression in Gliricidia, important resource populations of G. sepium on the Pacific coast appear to have retained their genetic integrity. Implications in terms of the conservation and utilization of genetic resources within the genus are discussed.

AB - Gliricidia sepium provides a variety of products important for rural communities in tropical countries. Native populations in Meso-America currently form an important source of seed for distribution to farmers, but concerns centre on mechanisms which may lead to their genetic erosion, including anthropogenic dispersal and subsequent introgression from the related species, G. maculata. Populations of Gliricidia were examined genetically using approaches based on the polymerase chain reaction to test for interspecific hybridization and introgression between G. sepium and G. maculata. Analysis involved 13 RAPD and two RFLP-PCR markers which were identified to have species-diagnostic distributions. Data from both approaches corresponded and indicated three locations where multilocus genotypes were consistent with an hybrid origin. Data at one of these sites was consistent with introgression following hybridization. The hybrid origin of populations was supported by the intermediate geographical location of these sites to 'pure' populations of each species. Analysis of maternally inherited organellar DNA, which involved the detection of SSCPs in mitochondrial DNA amplification products, allowed further delineation of genetic structure among Gliricidia populations. Mitochondrial data indicated a high degree of organelle differentiation between sampled locations and identified G. sepium- and G. maculatadiagnostic haplotypes. This data supported the interpretation of genetic structure based on RAPDs and RFLP-PCR. In addition, cytonuclear analysis allowed the directionality of gene transfer during the formation of hybrid populations to be described. Despite evidence for the occurrence of interspecific hybridization and introgression in Gliricidia, important resource populations of G. sepium on the Pacific coast appear to have retained their genetic integrity. Implications in terms of the conservation and utilization of genetic resources within the genus are discussed.

KW - G. maculata

KW - Genetic erosion

KW - Gliricidia sepium

KW - Interspecific gene flow

KW - Mitochondrial DNA

KW - RAPDs

KW - RFLPs

KW - SSCPs

UR - http://www.mendeley.com/research/detection-pattern-interspecific-hybridization-between-gliricidia-septum-g-maculata-mesoamerica-revea

U2 - 10.1111/j.1365-294X.1996.tb00294.x

DO - 10.1111/j.1365-294X.1996.tb00294.x

M3 - Article

VL - 5

SP - 89

EP - 98

JO - Molecular Ecology

JF - Molecular Ecology

SN - 0962-1083

IS - 1

ER -