Detection of extended‐spectrum β‐lactam, AmpC and carbapenem resistance in Enterobacteriaceae in beef cattle in Great Britain in 2015

Martina Velasova, RP Smith, Fabrizio Lemma, Robert A Horton, Nick Duggett, J Evans, SC Tongue, Muna F Anjum, Luke Randall*

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Aims: This study investigated the occurrence and genetic diversity of Enterobacteriaceae with Extended-Spectrum Beta-Lactamase (ESBL), AmpC and carbapenemase mediated resistance in British beef cattle, and related risk factors. Methods and Results: Faecal samples (n=776) were obtained from farms in England & Wales (n=20) and Scotland (n=20) in 2015. Isolates from selective agars were identified by MALDI ToF mass spectrometry. Selected isolates were characterised by multiplex PCR (blaCTX-M, blaOXA, blaSHV and blaTEM genes), whole genome sequencing (WGS), MICs and PFGE. None of the faecal samples yielded carbapenem resistant E. coli. Ten (25%) of the farms tested positive for ESBL-producing CTX-M Enterobacteriaceae, fifteen (37.5%) of the farms were positive for AmpC phenotype E. coli and none were positive for carbapenem resistant E. coli. WGS showed a total of 30 different resistance genes associated with E. coli, Citrobacter and Serratia from ESBL agars, and co-location of resistance genes with blaCTX-M1. Buying bulls and bringing in fattening cattle from another farm were identified as significant risk factors for positive samples harbouring CTX-M Enterobacteriaceae or AmpC phenotype E. coli respectively. Conclusions: Beef cattle on a proportion of farms in GB carry ESBL-producing Enterobacteriaceae. Factors, such as operating as a closed herd, may have an important role in reducing introduction and transmission of resistant Enterobacteriaceae. The results indicate management factors may play an important role in impacting ESBL prevalence. In particular, further study would be valuable to understand the impact of maintaining a closed herd on reducing the introduction of resistant Enterobacteriaceae. Significance and Impact of the study: This is the first study showing the presence of ESBL-producing Enterobacteriaceae in British beef cattle.
Original languageEnglish
Pages (from-to)1081-1095
Number of pages15
JournalJournal of Applied Microbiology
Volume126
Issue number4
Early online date28 Jan 2019
DOIs
Publication statusPrint publication - 1 Apr 2019

Fingerprint

Lactams
Carbapenems
Enterobacteriaceae
beta-Lactamases
Escherichia coli
Agar
Genome
Citrobacter
Genes
Serratia
Phenotype
Multiplex Polymerase Chain Reaction
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Wales
Scotland
United Kingdom
England
Mass Spectrometry
Farms

Keywords

  • Antibiotics
  • Enterobacteria
  • Epidemiology
  • Resistance
  • Veterinary

Cite this

Velasova, Martina ; Smith, RP ; Lemma, Fabrizio ; Horton, Robert A ; Duggett, Nick ; Evans, J ; Tongue, SC ; Anjum, Muna F ; Randall, Luke. / Detection of extended‐spectrum β‐lactam, AmpC and carbapenem resistance in Enterobacteriaceae in beef cattle in Great Britain in 2015. In: Journal of Applied Microbiology. 2019 ; Vol. 126, No. 4. pp. 1081-1095.
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abstract = "Aims: This study investigated the occurrence and genetic diversity of Enterobacteriaceae with Extended-Spectrum Beta-Lactamase (ESBL), AmpC and carbapenemase mediated resistance in British beef cattle, and related risk factors. Methods and Results: Faecal samples (n=776) were obtained from farms in England & Wales (n=20) and Scotland (n=20) in 2015. Isolates from selective agars were identified by MALDI ToF mass spectrometry. Selected isolates were characterised by multiplex PCR (blaCTX-M, blaOXA, blaSHV and blaTEM genes), whole genome sequencing (WGS), MICs and PFGE. None of the faecal samples yielded carbapenem resistant E. coli. Ten (25{\%}) of the farms tested positive for ESBL-producing CTX-M Enterobacteriaceae, fifteen (37.5{\%}) of the farms were positive for AmpC phenotype E. coli and none were positive for carbapenem resistant E. coli. WGS showed a total of 30 different resistance genes associated with E. coli, Citrobacter and Serratia from ESBL agars, and co-location of resistance genes with blaCTX-M1. Buying bulls and bringing in fattening cattle from another farm were identified as significant risk factors for positive samples harbouring CTX-M Enterobacteriaceae or AmpC phenotype E. coli respectively. Conclusions: Beef cattle on a proportion of farms in GB carry ESBL-producing Enterobacteriaceae. Factors, such as operating as a closed herd, may have an important role in reducing introduction and transmission of resistant Enterobacteriaceae. The results indicate management factors may play an important role in impacting ESBL prevalence. In particular, further study would be valuable to understand the impact of maintaining a closed herd on reducing the introduction of resistant Enterobacteriaceae. Significance and Impact of the study: This is the first study showing the presence of ESBL-producing Enterobacteriaceae in British beef cattle.",
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Detection of extended‐spectrum β‐lactam, AmpC and carbapenem resistance in Enterobacteriaceae in beef cattle in Great Britain in 2015. / Velasova, Martina; Smith, RP; Lemma, Fabrizio; Horton, Robert A; Duggett, Nick; Evans, J; Tongue, SC; Anjum, Muna F; Randall, Luke.

In: Journal of Applied Microbiology, Vol. 126, No. 4, 01.04.2019, p. 1081-1095.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Detection of extended‐spectrum β‐lactam, AmpC and carbapenem resistance in Enterobacteriaceae in beef cattle in Great Britain in 2015

AU - Velasova, Martina

AU - Smith, RP

AU - Lemma, Fabrizio

AU - Horton, Robert A

AU - Duggett, Nick

AU - Evans, J

AU - Tongue, SC

AU - Anjum, Muna F

AU - Randall, Luke

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Aims: This study investigated the occurrence and genetic diversity of Enterobacteriaceae with Extended-Spectrum Beta-Lactamase (ESBL), AmpC and carbapenemase mediated resistance in British beef cattle, and related risk factors. Methods and Results: Faecal samples (n=776) were obtained from farms in England & Wales (n=20) and Scotland (n=20) in 2015. Isolates from selective agars were identified by MALDI ToF mass spectrometry. Selected isolates were characterised by multiplex PCR (blaCTX-M, blaOXA, blaSHV and blaTEM genes), whole genome sequencing (WGS), MICs and PFGE. None of the faecal samples yielded carbapenem resistant E. coli. Ten (25%) of the farms tested positive for ESBL-producing CTX-M Enterobacteriaceae, fifteen (37.5%) of the farms were positive for AmpC phenotype E. coli and none were positive for carbapenem resistant E. coli. WGS showed a total of 30 different resistance genes associated with E. coli, Citrobacter and Serratia from ESBL agars, and co-location of resistance genes with blaCTX-M1. Buying bulls and bringing in fattening cattle from another farm were identified as significant risk factors for positive samples harbouring CTX-M Enterobacteriaceae or AmpC phenotype E. coli respectively. Conclusions: Beef cattle on a proportion of farms in GB carry ESBL-producing Enterobacteriaceae. Factors, such as operating as a closed herd, may have an important role in reducing introduction and transmission of resistant Enterobacteriaceae. The results indicate management factors may play an important role in impacting ESBL prevalence. In particular, further study would be valuable to understand the impact of maintaining a closed herd on reducing the introduction of resistant Enterobacteriaceae. Significance and Impact of the study: This is the first study showing the presence of ESBL-producing Enterobacteriaceae in British beef cattle.

AB - Aims: This study investigated the occurrence and genetic diversity of Enterobacteriaceae with Extended-Spectrum Beta-Lactamase (ESBL), AmpC and carbapenemase mediated resistance in British beef cattle, and related risk factors. Methods and Results: Faecal samples (n=776) were obtained from farms in England & Wales (n=20) and Scotland (n=20) in 2015. Isolates from selective agars were identified by MALDI ToF mass spectrometry. Selected isolates were characterised by multiplex PCR (blaCTX-M, blaOXA, blaSHV and blaTEM genes), whole genome sequencing (WGS), MICs and PFGE. None of the faecal samples yielded carbapenem resistant E. coli. Ten (25%) of the farms tested positive for ESBL-producing CTX-M Enterobacteriaceae, fifteen (37.5%) of the farms were positive for AmpC phenotype E. coli and none were positive for carbapenem resistant E. coli. WGS showed a total of 30 different resistance genes associated with E. coli, Citrobacter and Serratia from ESBL agars, and co-location of resistance genes with blaCTX-M1. Buying bulls and bringing in fattening cattle from another farm were identified as significant risk factors for positive samples harbouring CTX-M Enterobacteriaceae or AmpC phenotype E. coli respectively. Conclusions: Beef cattle on a proportion of farms in GB carry ESBL-producing Enterobacteriaceae. Factors, such as operating as a closed herd, may have an important role in reducing introduction and transmission of resistant Enterobacteriaceae. The results indicate management factors may play an important role in impacting ESBL prevalence. In particular, further study would be valuable to understand the impact of maintaining a closed herd on reducing the introduction of resistant Enterobacteriaceae. Significance and Impact of the study: This is the first study showing the presence of ESBL-producing Enterobacteriaceae in British beef cattle.

KW - Antibiotics

KW - Enterobacteria

KW - Epidemiology

KW - Resistance

KW - Veterinary

U2 - 10.1111/jam.14211

DO - 10.1111/jam.14211

M3 - Article

C2 - 30693606

VL - 126

SP - 1081

EP - 1095

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 4

ER -