Abstract
During 2009-2013, 302 single-spore isolates of Botrytis cinerea were collected from vineyards located in the most important site of table grape production in Sicily, recognized by the European Community as Protected Geographical Indication (PGI) ‘Mazzarrone grape’. In preliminary studies, all isolates were
tested in vitro for their sensitivity to six fungicides belonging to the following groups: benzimidazoles,
dicarboximides, anilinopyrimidines, succinate dehydrogenase inhibitors, hydroxyanilides and phenylpyrroles.
In these tests, 45.7% of the isolates were found to be resistant to at least one fungicide. Specific
resistance to pyrimethanil was found in 30.8% of the isolates, whereas 13.9, 10.3 and 7.6% of the isolates
exhibited resistance to carbendazim, iprodione and boscalid, respectively. No isolates resistant to fenhexamid
and fludioxonil were detected within our dataset of B. cinerea isolates. However, 30 B. cinerea
isolates possessed multiple resistance to two or more fungicides. In detail, 8 isolates were simultaneously
resistant to four fungicides, whereas 5 and 17 isolates were resistant to three and two fungicides,
respectively. For boscalid, 11/23 of isolates showing in vitro resistance possessed a mutation at the SdhB
gene, whereas all isolates resistant to carbendazim and iprodione possessed mutations at b-tubulin and
BcOS1 histidine kinase genes, respectively. Accordingly, these fungicides failed to control gray mould
infections caused by resistant or reduced sensitivity isolates on grape berries and grapevine leaves
whereas the sensitive isolates were effectively managed by all fungicides applied at label rates. This study
represents the first report of B. cinerea field isolates resistant and/or with simultaneous resistance to
several botryticides from table grape vineyards in Sicily. Therefore, current strategies for fungicide
resistance management of B. cinerea could be negatively affected in future.
© 2015 Elsevier Ltd. All rights reserved.
Original language | English |
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Pages (from-to) | 65 - 73 |
Number of pages | 9 |
Journal | Crop Protection |
Volume | 77 |
DOIs | |
Publication status | Print publication - 2015 |
Bibliographical note
1023107Keywords
- Boscalid
- Botrytis cinerea
- Multiple fungicide resistance
- Table grape