Detection of Botrytis cinerea field isolates with multiple fungicide resistance from table grape in Sicily

A Panebianco, I Castello, G Cirvilleri, G Perrone, F Epifani, M Ferrara, G Polizzi, DR Walters, A Vitale

Research output: Contribution to journalArticle

35 Citations (Scopus)
25 Downloads (Pure)

Abstract

During 2009-2013, 302 single-spore isolates of Botrytis cinerea were collected from vineyards located in the most important site of table grape production in Sicily, recognized by the European Community as Protected Geographical Indication (PGI) ‘Mazzarrone grape’. In preliminary studies, all isolates were tested in vitro for their sensitivity to six fungicides belonging to the following groups: benzimidazoles, dicarboximides, anilinopyrimidines, succinate dehydrogenase inhibitors, hydroxyanilides and phenylpyrroles. In these tests, 45.7% of the isolates were found to be resistant to at least one fungicide. Specific resistance to pyrimethanil was found in 30.8% of the isolates, whereas 13.9, 10.3 and 7.6% of the isolates exhibited resistance to carbendazim, iprodione and boscalid, respectively. No isolates resistant to fenhexamid and fludioxonil were detected within our dataset of B. cinerea isolates. However, 30 B. cinerea isolates possessed multiple resistance to two or more fungicides. In detail, 8 isolates were simultaneously resistant to four fungicides, whereas 5 and 17 isolates were resistant to three and two fungicides, respectively. For boscalid, 11/23 of isolates showing in vitro resistance possessed a mutation at the SdhB gene, whereas all isolates resistant to carbendazim and iprodione possessed mutations at b-tubulin and BcOS1 histidine kinase genes, respectively. Accordingly, these fungicides failed to control gray mould infections caused by resistant or reduced sensitivity isolates on grape berries and grapevine leaves whereas the sensitive isolates were effectively managed by all fungicides applied at label rates. This study represents the first report of B. cinerea field isolates resistant and/or with simultaneous resistance to several botryticides from table grape vineyards in Sicily. Therefore, current strategies for fungicide resistance management of B. cinerea could be negatively affected in future. © 2015 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)65 - 73
Number of pages9
JournalCrop Protection
Volume77
DOIs
Publication statusPrint publication - 2015

Fingerprint

fungicide resistance
table grapes
Sicily
Botrytis cinerea
fungicides
boscalid
iprodione
carbendazim
vineyards
succinate dehydrogenase (quinone)
grapes
protected designation of origin
benzimidazoles
fludioxonil
histidine kinase
mutation
viticulture
tubulin
spores

Bibliographical note

1023107

Keywords

  • Boscalid
  • Botrytis cinerea
  • Multiple fungicide resistance
  • Table grape

Cite this

Panebianco, A., Castello, I., Cirvilleri, G., Perrone, G., Epifani, F., Ferrara, M., ... Vitale, A. (2015). Detection of Botrytis cinerea field isolates with multiple fungicide resistance from table grape in Sicily. Crop Protection, 77, 65 - 73. https://doi.org/10.1016/j.cropro.2015.07.010
Panebianco, A ; Castello, I ; Cirvilleri, G ; Perrone, G ; Epifani, F ; Ferrara, M ; Polizzi, G ; Walters, DR ; Vitale, A. / Detection of Botrytis cinerea field isolates with multiple fungicide resistance from table grape in Sicily. In: Crop Protection. 2015 ; Vol. 77. pp. 65 - 73.
@article{745586540882460a9c0a4026ee5545bc,
title = "Detection of Botrytis cinerea field isolates with multiple fungicide resistance from table grape in Sicily",
abstract = "During 2009-2013, 302 single-spore isolates of Botrytis cinerea were collected from vineyards located in the most important site of table grape production in Sicily, recognized by the European Community as Protected Geographical Indication (PGI) ‘Mazzarrone grape’. In preliminary studies, all isolates were tested in vitro for their sensitivity to six fungicides belonging to the following groups: benzimidazoles, dicarboximides, anilinopyrimidines, succinate dehydrogenase inhibitors, hydroxyanilides and phenylpyrroles. In these tests, 45.7{\%} of the isolates were found to be resistant to at least one fungicide. Specific resistance to pyrimethanil was found in 30.8{\%} of the isolates, whereas 13.9, 10.3 and 7.6{\%} of the isolates exhibited resistance to carbendazim, iprodione and boscalid, respectively. No isolates resistant to fenhexamid and fludioxonil were detected within our dataset of B. cinerea isolates. However, 30 B. cinerea isolates possessed multiple resistance to two or more fungicides. In detail, 8 isolates were simultaneously resistant to four fungicides, whereas 5 and 17 isolates were resistant to three and two fungicides, respectively. For boscalid, 11/23 of isolates showing in vitro resistance possessed a mutation at the SdhB gene, whereas all isolates resistant to carbendazim and iprodione possessed mutations at b-tubulin and BcOS1 histidine kinase genes, respectively. Accordingly, these fungicides failed to control gray mould infections caused by resistant or reduced sensitivity isolates on grape berries and grapevine leaves whereas the sensitive isolates were effectively managed by all fungicides applied at label rates. This study represents the first report of B. cinerea field isolates resistant and/or with simultaneous resistance to several botryticides from table grape vineyards in Sicily. Therefore, current strategies for fungicide resistance management of B. cinerea could be negatively affected in future. {\circledC} 2015 Elsevier Ltd. All rights reserved.",
keywords = "Boscalid, Botrytis cinerea, Multiple fungicide resistance, Table grape",
author = "A Panebianco and I Castello and G Cirvilleri and G Perrone and F Epifani and M Ferrara and G Polizzi and DR Walters and A Vitale",
note = "1023107",
year = "2015",
doi = "10.1016/j.cropro.2015.07.010",
language = "English",
volume = "77",
pages = "65 -- 73",
journal = "Crop Protection",

}

Panebianco, A, Castello, I, Cirvilleri, G, Perrone, G, Epifani, F, Ferrara, M, Polizzi, G, Walters, DR & Vitale, A 2015, 'Detection of Botrytis cinerea field isolates with multiple fungicide resistance from table grape in Sicily', Crop Protection, vol. 77, pp. 65 - 73. https://doi.org/10.1016/j.cropro.2015.07.010

Detection of Botrytis cinerea field isolates with multiple fungicide resistance from table grape in Sicily. / Panebianco, A; Castello, I; Cirvilleri, G; Perrone, G; Epifani, F; Ferrara, M; Polizzi, G; Walters, DR; Vitale, A.

In: Crop Protection, Vol. 77, 2015, p. 65 - 73.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Detection of Botrytis cinerea field isolates with multiple fungicide resistance from table grape in Sicily

AU - Panebianco, A

AU - Castello, I

AU - Cirvilleri, G

AU - Perrone, G

AU - Epifani, F

AU - Ferrara, M

AU - Polizzi, G

AU - Walters, DR

AU - Vitale, A

N1 - 1023107

PY - 2015

Y1 - 2015

N2 - During 2009-2013, 302 single-spore isolates of Botrytis cinerea were collected from vineyards located in the most important site of table grape production in Sicily, recognized by the European Community as Protected Geographical Indication (PGI) ‘Mazzarrone grape’. In preliminary studies, all isolates were tested in vitro for their sensitivity to six fungicides belonging to the following groups: benzimidazoles, dicarboximides, anilinopyrimidines, succinate dehydrogenase inhibitors, hydroxyanilides and phenylpyrroles. In these tests, 45.7% of the isolates were found to be resistant to at least one fungicide. Specific resistance to pyrimethanil was found in 30.8% of the isolates, whereas 13.9, 10.3 and 7.6% of the isolates exhibited resistance to carbendazim, iprodione and boscalid, respectively. No isolates resistant to fenhexamid and fludioxonil were detected within our dataset of B. cinerea isolates. However, 30 B. cinerea isolates possessed multiple resistance to two or more fungicides. In detail, 8 isolates were simultaneously resistant to four fungicides, whereas 5 and 17 isolates were resistant to three and two fungicides, respectively. For boscalid, 11/23 of isolates showing in vitro resistance possessed a mutation at the SdhB gene, whereas all isolates resistant to carbendazim and iprodione possessed mutations at b-tubulin and BcOS1 histidine kinase genes, respectively. Accordingly, these fungicides failed to control gray mould infections caused by resistant or reduced sensitivity isolates on grape berries and grapevine leaves whereas the sensitive isolates were effectively managed by all fungicides applied at label rates. This study represents the first report of B. cinerea field isolates resistant and/or with simultaneous resistance to several botryticides from table grape vineyards in Sicily. Therefore, current strategies for fungicide resistance management of B. cinerea could be negatively affected in future. © 2015 Elsevier Ltd. All rights reserved.

AB - During 2009-2013, 302 single-spore isolates of Botrytis cinerea were collected from vineyards located in the most important site of table grape production in Sicily, recognized by the European Community as Protected Geographical Indication (PGI) ‘Mazzarrone grape’. In preliminary studies, all isolates were tested in vitro for their sensitivity to six fungicides belonging to the following groups: benzimidazoles, dicarboximides, anilinopyrimidines, succinate dehydrogenase inhibitors, hydroxyanilides and phenylpyrroles. In these tests, 45.7% of the isolates were found to be resistant to at least one fungicide. Specific resistance to pyrimethanil was found in 30.8% of the isolates, whereas 13.9, 10.3 and 7.6% of the isolates exhibited resistance to carbendazim, iprodione and boscalid, respectively. No isolates resistant to fenhexamid and fludioxonil were detected within our dataset of B. cinerea isolates. However, 30 B. cinerea isolates possessed multiple resistance to two or more fungicides. In detail, 8 isolates were simultaneously resistant to four fungicides, whereas 5 and 17 isolates were resistant to three and two fungicides, respectively. For boscalid, 11/23 of isolates showing in vitro resistance possessed a mutation at the SdhB gene, whereas all isolates resistant to carbendazim and iprodione possessed mutations at b-tubulin and BcOS1 histidine kinase genes, respectively. Accordingly, these fungicides failed to control gray mould infections caused by resistant or reduced sensitivity isolates on grape berries and grapevine leaves whereas the sensitive isolates were effectively managed by all fungicides applied at label rates. This study represents the first report of B. cinerea field isolates resistant and/or with simultaneous resistance to several botryticides from table grape vineyards in Sicily. Therefore, current strategies for fungicide resistance management of B. cinerea could be negatively affected in future. © 2015 Elsevier Ltd. All rights reserved.

KW - Boscalid

KW - Botrytis cinerea

KW - Multiple fungicide resistance

KW - Table grape

U2 - 10.1016/j.cropro.2015.07.010

DO - 10.1016/j.cropro.2015.07.010

M3 - Article

VL - 77

SP - 65

EP - 73

JO - Crop Protection

JF - Crop Protection

ER -