Detection of Neospora caninum DNA in cases of bovine and ovine abortion in the South-West of Scotland

P M Bartley, S Guido, C Mason, H Stevenson, F Chianini, H Carty, E A Innes, F Katzer

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Neospora caninum is a commonly diagnosed cause of reproductive losses in farmed ruminants worldwide. This study examined 495 and 308 samples (brain, heart and placenta) which were collected from 455 and 119 aborted cattle and sheep fetuses, respectively. DNA was extracted and a nested Neospora ITS1 PCR was performed on all samples. The results showed that for bovine fetuses 79/449 brain [17.6% (14.2-21.4)], 7/25 heart [28.0% (12.1-49.4)] and 5/21 placenta [23.8% (8.2-47.2)] were PCR positive for the presence of Neospora DNA. Overall 82/455 [18.0% (14.6-21.7)] of the bovine fetuses tested positive for the presence of N. caninum DNA in at least one sample. None (0/308) of the ovine fetal samples tested positive for the presence of Neospora DNA in any of the tissues tested. The results show that N. caninum was associated with fetal losses in cattle (distributed across South-West Scotland), compared to sheep in the same geographical areas where no parasite DNA was found. Neospora is well distributed amongst cattle in South-West Scotland and is the potential cause of serious economic losses to the Scottish cattle farming community; however, it does not appear to be a problem amongst the Scottish sheep flocks.

Original languageEnglish
Pages (from-to)979-982
Number of pages4
JournalParasitology
Volume146
Issue number7
Early online date12 Apr 2019
DOIs
Publication statusPrint publication - Jun 2019

Fingerprint

Neospora
abortion (animals)
Neospora caninum
Scotland
Sheep
sheep
cattle
DNA
fetus
Fetus
placenta
Placenta
heart
brain
sampling
Polymerase Chain Reaction
Brain
Ruminants
Agriculture
ruminants

Bibliographical note

© Cambridge University Press 2019

Keywords

  • Abortion
  • Bovine
  • Cattle
  • DNA
  • Neospora caninum
  • Ovine
  • Scotland
  • Sheep

Cite this

Bartley, P. M., Guido, S., Mason, C., Stevenson, H., Chianini, F., Carty, H., ... Katzer, F. (2019). Detection of Neospora caninum DNA in cases of bovine and ovine abortion in the South-West of Scotland. Parasitology, 146(7), 979-982. https://doi.org/10.1017/S0031182019000301
Bartley, P M ; Guido, S ; Mason, C ; Stevenson, H ; Chianini, F ; Carty, H ; Innes, E A ; Katzer, F. / Detection of Neospora caninum DNA in cases of bovine and ovine abortion in the South-West of Scotland. In: Parasitology. 2019 ; Vol. 146, No. 7. pp. 979-982.
@article{2ff3aead133c4c938ee804a8d4bd626d,
title = "Detection of Neospora caninum DNA in cases of bovine and ovine abortion in the South-West of Scotland",
abstract = "Neospora caninum is a commonly diagnosed cause of reproductive losses in farmed ruminants worldwide. This study examined 495 and 308 samples (brain, heart and placenta) which were collected from 455 and 119 aborted cattle and sheep fetuses, respectively. DNA was extracted and a nested Neospora ITS1 PCR was performed on all samples. The results showed that for bovine fetuses 79/449 brain [17.6{\%} (14.2-21.4)], 7/25 heart [28.0{\%} (12.1-49.4)] and 5/21 placenta [23.8{\%} (8.2-47.2)] were PCR positive for the presence of Neospora DNA. Overall 82/455 [18.0{\%} (14.6-21.7)] of the bovine fetuses tested positive for the presence of N. caninum DNA in at least one sample. None (0/308) of the ovine fetal samples tested positive for the presence of Neospora DNA in any of the tissues tested. The results show that N. caninum was associated with fetal losses in cattle (distributed across South-West Scotland), compared to sheep in the same geographical areas where no parasite DNA was found. Neospora is well distributed amongst cattle in South-West Scotland and is the potential cause of serious economic losses to the Scottish cattle farming community; however, it does not appear to be a problem amongst the Scottish sheep flocks.",
keywords = "Abortion, Bovine, Cattle, DNA, Neospora caninum, Ovine, Scotland, Sheep",
author = "Bartley, {P M} and S Guido and C Mason and H Stevenson and F Chianini and H Carty and Innes, {E A} and F Katzer",
note = "{\circledC} Cambridge University Press 2019",
year = "2019",
month = "6",
doi = "10.1017/S0031182019000301",
language = "English",
volume = "146",
pages = "979--982",
journal = "Parasitology",
issn = "0031-1820",
publisher = "Cambridge University Press",
number = "7",

}

Bartley, PM, Guido, S, Mason, C, Stevenson, H, Chianini, F, Carty, H, Innes, EA & Katzer, F 2019, 'Detection of Neospora caninum DNA in cases of bovine and ovine abortion in the South-West of Scotland', Parasitology, vol. 146, no. 7, pp. 979-982. https://doi.org/10.1017/S0031182019000301

Detection of Neospora caninum DNA in cases of bovine and ovine abortion in the South-West of Scotland. / Bartley, P M; Guido, S; Mason, C; Stevenson, H; Chianini, F; Carty, H; Innes, E A; Katzer, F.

In: Parasitology, Vol. 146, No. 7, 06.2019, p. 979-982.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Detection of Neospora caninum DNA in cases of bovine and ovine abortion in the South-West of Scotland

AU - Bartley, P M

AU - Guido, S

AU - Mason, C

AU - Stevenson, H

AU - Chianini, F

AU - Carty, H

AU - Innes, E A

AU - Katzer, F

N1 - © Cambridge University Press 2019

PY - 2019/6

Y1 - 2019/6

N2 - Neospora caninum is a commonly diagnosed cause of reproductive losses in farmed ruminants worldwide. This study examined 495 and 308 samples (brain, heart and placenta) which were collected from 455 and 119 aborted cattle and sheep fetuses, respectively. DNA was extracted and a nested Neospora ITS1 PCR was performed on all samples. The results showed that for bovine fetuses 79/449 brain [17.6% (14.2-21.4)], 7/25 heart [28.0% (12.1-49.4)] and 5/21 placenta [23.8% (8.2-47.2)] were PCR positive for the presence of Neospora DNA. Overall 82/455 [18.0% (14.6-21.7)] of the bovine fetuses tested positive for the presence of N. caninum DNA in at least one sample. None (0/308) of the ovine fetal samples tested positive for the presence of Neospora DNA in any of the tissues tested. The results show that N. caninum was associated with fetal losses in cattle (distributed across South-West Scotland), compared to sheep in the same geographical areas where no parasite DNA was found. Neospora is well distributed amongst cattle in South-West Scotland and is the potential cause of serious economic losses to the Scottish cattle farming community; however, it does not appear to be a problem amongst the Scottish sheep flocks.

AB - Neospora caninum is a commonly diagnosed cause of reproductive losses in farmed ruminants worldwide. This study examined 495 and 308 samples (brain, heart and placenta) which were collected from 455 and 119 aborted cattle and sheep fetuses, respectively. DNA was extracted and a nested Neospora ITS1 PCR was performed on all samples. The results showed that for bovine fetuses 79/449 brain [17.6% (14.2-21.4)], 7/25 heart [28.0% (12.1-49.4)] and 5/21 placenta [23.8% (8.2-47.2)] were PCR positive for the presence of Neospora DNA. Overall 82/455 [18.0% (14.6-21.7)] of the bovine fetuses tested positive for the presence of N. caninum DNA in at least one sample. None (0/308) of the ovine fetal samples tested positive for the presence of Neospora DNA in any of the tissues tested. The results show that N. caninum was associated with fetal losses in cattle (distributed across South-West Scotland), compared to sheep in the same geographical areas where no parasite DNA was found. Neospora is well distributed amongst cattle in South-West Scotland and is the potential cause of serious economic losses to the Scottish cattle farming community; however, it does not appear to be a problem amongst the Scottish sheep flocks.

KW - Abortion

KW - Bovine

KW - Cattle

KW - DNA

KW - Neospora caninum

KW - Ovine

KW - Scotland

KW - Sheep

U2 - 10.1017/S0031182019000301

DO - 10.1017/S0031182019000301

M3 - Article

C2 - 30975236

VL - 146

SP - 979

EP - 982

JO - Parasitology

JF - Parasitology

SN - 0031-1820

IS - 7

ER -