Development of a loop-mediated isothermal amplification (LAMP) assay for rapid and sensitive identification of Arcanobacterium pluranimalium

A Abdulmawjood, J Wickhorst, O Sammra, C Lammler, G Foster, PN Wragg, E Prenger-Berninghoff, G Klein

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In the present study 28 Arcanobacterium pluranimalium strains isolated from various origins could successfully be identified with a newly designed loop-mediated isothermal amplification (LAMP) assay based on gene pla encoding pluranimaliumlysin. No comparable reaction could be observed with control strains representing five species of genus Arcanobacterium and five species of genus Trueperella. The presented pla LAMP assay might allow a reliable and low cost identification of this bacterial pathogen also in laboratories with less specified equipment. © 2015 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)468 - 472
Number of pages5
JournalMolecular and Cellular Probes
Volume29
Early online date18 Jun 2015
DOIs
Publication statusFirst published - 18 Jun 2015

Keywords

  • Arcanobacterium pluranimalium
  • Loop-mediated isothermal amplification
  • Pla
  • Pluranimaliumlysin

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