Development of a loop-mediated isothermal amplification (LAMP) assay for rapid and sensitive identification of Arcanobacterium pluranimalium

A Abdulmawjood, J Wickhorst, O Sammra, C Lammler, G Foster, PN Wragg, E Prenger-Berninghoff, G Klein

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

Abstract

In the present study 28 Arcanobacterium pluranimalium strains isolated from various origins could successfully be identified with a newly designed loop-mediated isothermal amplification (LAMP) assay based on gene pla encoding pluranimaliumlysin. No comparable reaction could be observed with control strains representing five species of genus Arcanobacterium and five species of genus Trueperella. The presented pla LAMP assay might allow a reliable and low cost identification of this bacterial pathogen also in laboratories with less specified equipment. © 2015 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)468 - 472
Number of pages5
JournalMolecular and Cellular Probes
Volume29
DOIs
Publication statusFirst published - 18 Jun 2015

Fingerprint

Arcanobacterium pluranimalium
Arcanobacterium
assays
genes
loop-mediated isothermal amplification

Keywords

  • Arcanobacterium pluranimalium
  • Loop-mediated isothermal amplification
  • Pla
  • Pluranimaliumlysin

Cite this

Abdulmawjood, A ; Wickhorst, J ; Sammra, O ; Lammler, C ; Foster, G ; Wragg, PN ; Prenger-Berninghoff, E ; Klein, G. / Development of a loop-mediated isothermal amplification (LAMP) assay for rapid and sensitive identification of Arcanobacterium pluranimalium. In: Molecular and Cellular Probes. 2015 ; Vol. 29. pp. 468 - 472.
@article{cf3e7a2ef0cf481d80aef53bfac91284,
title = "Development of a loop-mediated isothermal amplification (LAMP) assay for rapid and sensitive identification of Arcanobacterium pluranimalium",
abstract = "In the present study 28 Arcanobacterium pluranimalium strains isolated from various origins could successfully be identified with a newly designed loop-mediated isothermal amplification (LAMP) assay based on gene pla encoding pluranimaliumlysin. No comparable reaction could be observed with control strains representing five species of genus Arcanobacterium and five species of genus Trueperella. The presented pla LAMP assay might allow a reliable and low cost identification of this bacterial pathogen also in laboratories with less specified equipment. {\circledC} 2015 Elsevier Ltd. All rights reserved.",
keywords = "Arcanobacterium pluranimalium, Loop-mediated isothermal amplification, Pla, Pluranimaliumlysin",
author = "A Abdulmawjood and J Wickhorst and O Sammra and C Lammler and G Foster and PN Wragg and E Prenger-Berninghoff and G Klein",
year = "2015",
month = "6",
day = "18",
doi = "10.1016/j.mcp.2015.06.003",
language = "English",
volume = "29",
pages = "468 -- 472",
journal = "Molecular and Cellular Probes",
issn = "0890-8508",
publisher = "Elsevier",

}

Development of a loop-mediated isothermal amplification (LAMP) assay for rapid and sensitive identification of Arcanobacterium pluranimalium. / Abdulmawjood, A; Wickhorst, J; Sammra, O; Lammler, C; Foster, G; Wragg, PN; Prenger-Berninghoff, E; Klein, G.

In: Molecular and Cellular Probes, Vol. 29, 18.06.2015, p. 468 - 472.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Development of a loop-mediated isothermal amplification (LAMP) assay for rapid and sensitive identification of Arcanobacterium pluranimalium

AU - Abdulmawjood, A

AU - Wickhorst, J

AU - Sammra, O

AU - Lammler, C

AU - Foster, G

AU - Wragg, PN

AU - Prenger-Berninghoff, E

AU - Klein, G

PY - 2015/6/18

Y1 - 2015/6/18

N2 - In the present study 28 Arcanobacterium pluranimalium strains isolated from various origins could successfully be identified with a newly designed loop-mediated isothermal amplification (LAMP) assay based on gene pla encoding pluranimaliumlysin. No comparable reaction could be observed with control strains representing five species of genus Arcanobacterium and five species of genus Trueperella. The presented pla LAMP assay might allow a reliable and low cost identification of this bacterial pathogen also in laboratories with less specified equipment. © 2015 Elsevier Ltd. All rights reserved.

AB - In the present study 28 Arcanobacterium pluranimalium strains isolated from various origins could successfully be identified with a newly designed loop-mediated isothermal amplification (LAMP) assay based on gene pla encoding pluranimaliumlysin. No comparable reaction could be observed with control strains representing five species of genus Arcanobacterium and five species of genus Trueperella. The presented pla LAMP assay might allow a reliable and low cost identification of this bacterial pathogen also in laboratories with less specified equipment. © 2015 Elsevier Ltd. All rights reserved.

KW - Arcanobacterium pluranimalium

KW - Loop-mediated isothermal amplification

KW - Pla

KW - Pluranimaliumlysin

U2 - 10.1016/j.mcp.2015.06.003

DO - 10.1016/j.mcp.2015.06.003

M3 - Article

VL - 29

SP - 468

EP - 472

JO - Molecular and Cellular Probes

JF - Molecular and Cellular Probes

SN - 0890-8508

ER -