Development of a loop-mediated isothermal amplification (LAMP) assay for rapid and sensitive identification of Arcanobacterium pluranimalium

A Abdulmawjood; J Wickhorst; O Sammra; C Lammler; G Foster; PN Wragg; E Prenger-Berninghoff; G Klein

doi:10.1016/j.mcp.2015.06.003

Development of a loop-mediated isothermal amplification (LAMP) assay for rapid and sensitive identification of Arcanobacterium pluranimalium

A Abdulmawjood, J Wickhorst, O Sammra, C Lammler, G Foster, PN Wragg, E Prenger-Berninghoff, G Klein

Veterinary Services

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Abstract

In the present study 28 Arcanobacterium pluranimalium strains isolated from various origins could successfully be identified with a newly designed loop-mediated isothermal amplification (LAMP) assay based on gene pla encoding pluranimaliumlysin. No comparable reaction could be observed with control strains representing five species of genus Arcanobacterium and five species of genus Trueperella. The presented pla LAMP assay might allow a reliable and low cost identification of this bacterial pathogen also in laboratories with less specified equipment. © 2015 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	468 - 472
Number of pages	5
Journal	Molecular and Cellular Probes
Volume	29
Early online date	18 Jun 2015
DOIs	https://doi.org/10.1016/j.mcp.2015.06.003
Publication status	First published - 18 Jun 2015

Keywords

Arcanobacterium pluranimalium
Loop-mediated isothermal amplification
Pla
Pluranimaliumlysin

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10.1016/j.mcp.2015.06.003

A pluranimalium LAMP18.05.2015 lAccepted author manuscript, 44.3 KBLicence: CC BY-NC-ND

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Abdulmawjood, A., Wickhorst, J., Sammra, O., Lammler, C., Foster, G., Wragg, PN., Prenger-Berninghoff, E., & Klein, G. (2015). Development of a loop-mediated isothermal amplification (LAMP) assay for rapid and sensitive identification of Arcanobacterium pluranimalium. Molecular and Cellular Probes, 29, 468 - 472. Advance online publication. https://doi.org/10.1016/j.mcp.2015.06.003

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AU - Abdulmawjood, A

AU - Wickhorst, J

AU - Sammra, O

AU - Lammler, C

AU - Foster, G

AU - Wragg, PN

AU - Prenger-Berninghoff, E

AU - Klein, G

PY - 2015/6/18

Y1 - 2015/6/18

N2 - In the present study 28 Arcanobacterium pluranimalium strains isolated from various origins could successfully be identified with a newly designed loop-mediated isothermal amplification (LAMP) assay based on gene pla encoding pluranimaliumlysin. No comparable reaction could be observed with control strains representing five species of genus Arcanobacterium and five species of genus Trueperella. The presented pla LAMP assay might allow a reliable and low cost identification of this bacterial pathogen also in laboratories with less specified equipment. © 2015 Elsevier Ltd. All rights reserved.

AB - In the present study 28 Arcanobacterium pluranimalium strains isolated from various origins could successfully be identified with a newly designed loop-mediated isothermal amplification (LAMP) assay based on gene pla encoding pluranimaliumlysin. No comparable reaction could be observed with control strains representing five species of genus Arcanobacterium and five species of genus Trueperella. The presented pla LAMP assay might allow a reliable and low cost identification of this bacterial pathogen also in laboratories with less specified equipment. © 2015 Elsevier Ltd. All rights reserved.

KW - Arcanobacterium pluranimalium

KW - Loop-mediated isothermal amplification

KW - Pla

KW - Pluranimaliumlysin

U2 - 10.1016/j.mcp.2015.06.003

DO - 10.1016/j.mcp.2015.06.003

M3 - Article

SN - 0890-8508

VL - 29

SP - 468

EP - 472

JO - Molecular and Cellular Probes

JF - Molecular and Cellular Probes

ER -

Development of a loop-mediated isothermal amplification (LAMP) assay for rapid and sensitive identification of Arcanobacterium pluranimalium

Abstract

Keywords

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