Development of a loop-mediated isothermal amplification (LAMP) assay for molecular identification of Trueperella abortisuis isolated from pigs

Marwa F.E. Ahmed, Mazen Alssahen, Christoph Lämmler, Geoffrey Foster, Antonia Kreitlow, Isabel Hennig-Pauka, Madeleine Plötz, Amir Abdulmawjood*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The first description of Trueperella (T.) abortisuis was presented in Japan in 2009 by Azuma and colleagues. In the current study, eight T. abortisuis strains were identified by a newly developed loop-mediated isothermal amplification (LAMP) assay based on the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) encoding gene gap. Two T. abortisuis strains were obtained from prepuce of a seven-month-old boar and pooled foetal stomach contents in the United Kingdom, while the other six T. abortisuis strains were recovered from aborted foetal material of six pigs from a single farm in Germany. The developed LAMP assay showed an analytical sensitivity of 22 pg μL-1 T. abortisuis DNA. T. abortisuis DSM 19515T and field strain T. abortisuis P504054/19/1 were directly detectable in artificially contaminated vaginal swabs up to concentrations of 980 CFU and 770 CFU per swab, respectively. There was no cross reactivity with control strains representing six species of genus Trueperella and six species of the closely related genus Arcanobacterium and Schaalia (Actinomyces) hyovaginalis. Further field research is required to determine the usefulness of the designed LAMP assay for identifying T. abortisuis isolated from pigs of various origins and from test samples directly obtained at farm level.

Original languageEnglish
Article number101795
JournalMolecular and Cellular Probes
Volume62
Early online date4 Feb 2022
DOIs
Publication statusPrint publication - Apr 2022

Bibliographical note

Publisher Copyright: © 2022 Elsevier Ltd

Keywords

  • gap
  • LAMP assay
  • Pig
  • Trueperella abortisuis
  • Arcanobacterium/genetics
  • Actinomycetaceae
  • RNA, Ribosomal, 16S/genetics
  • Male
  • Nucleic Acid Amplification Techniques
  • Molecular Diagnostic Techniques
  • Animals
  • Swine
  • Sensitivity and Specificity
  • Female

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