TY - JOUR
T1 - Development of a real time reverse transcriptase polymerase chain reaction for the detection of bovine respiratory syncytial virus in clinical samples and its comparison with immunohistochemistry and immunofluorescence antibody testing
AU - Willoughby, Kim
AU - Thomson, Karen
AU - Maley, Madeleine
AU - Gilray, Janice
AU - Scholes, Sandra
AU - Howie, Fiona
AU - Caldow, George
AU - Nettleton, Peter F
PY - 2008/1/1
Y1 - 2008/1/1
N2 - Bovine respiratory syncytial virus is an agent involved in calf pneumonia complex, a disease of significant economic importance. Accurate diagnosis of the agents involved on farm premises is important when formulating disease control measures, including vaccination. We have developed a real time reverse transcriptase polymerase chain reaction (rtRT-PCR) and compared it with the diagnostic tests currently available in the United Kingdom: immunohistochemistry (IHC) and immunofluorescence antibody test (IFAT). The rtRT-PCR had a detection limit of 10 gene copies and was 96% efficient. Recent UK isolates and clinical samples were tested; the rtRT-PCR was more sensitive than both conventional tests.
AB - Bovine respiratory syncytial virus is an agent involved in calf pneumonia complex, a disease of significant economic importance. Accurate diagnosis of the agents involved on farm premises is important when formulating disease control measures, including vaccination. We have developed a real time reverse transcriptase polymerase chain reaction (rtRT-PCR) and compared it with the diagnostic tests currently available in the United Kingdom: immunohistochemistry (IHC) and immunofluorescence antibody test (IFAT). The rtRT-PCR had a detection limit of 10 gene copies and was 96% efficient. Recent UK isolates and clinical samples were tested; the rtRT-PCR was more sensitive than both conventional tests.
KW - Animals
KW - Cattle
KW - Cattle Diseases/diagnosis
KW - Fluorescent Antibody Technique, Direct/veterinary
KW - Immunohistochemistry/veterinary
KW - Respiratory Syncytial Virus Infections/diagnosis
KW - Respiratory Syncytial Virus, Bovine/genetics
KW - Reverse Transcriptase Polymerase Chain Reaction/veterinary
U2 - 10.1016/j.vetmic.2007.07.005
DO - 10.1016/j.vetmic.2007.07.005
M3 - Short communication
C2 - 17709212
VL - 126
SP - 264
EP - 270
JO - Veterinary Microbiology
JF - Veterinary Microbiology
SN - 0378-1135
IS - 1-3
ER -