Bovine respiratory syncytial virus is an agent involved in calf pneumonia complex, a disease of significant economic importance. Accurate diagnosis of the agents involved on farm premises is important when formulating disease control measures, including vaccination. We have developed a real time reverse transcriptase polymerase chain reaction (rtRT-PCR) and compared it with the diagnostic tests currently available in the United Kingdom: immunohistochemistry (IHC) and immunofluorescence antibody test (IFAT). The rtRT-PCR had a detection limit of 10 gene copies and was 96% efficient. Recent UK isolates and clinical samples were tested; the rtRT-PCR was more sensitive than both conventional tests.
- Cattle Diseases/diagnosis
- Fluorescent Antibody Technique, Direct/veterinary
- Respiratory Syncytial Virus Infections/diagnosis
- Respiratory Syncytial Virus, Bovine/genetics
- Reverse Transcriptase Polymerase Chain Reaction/veterinary