Development of a real time reverse transcriptase polymerase chain reaction for the detection of bovine respiratory syncytial virus in clinical samples and its comparison with immunohistochemistry and immunofluorescence antibody testing

Kim Willoughby; Karen Thomson; Madeleine Maley; Janice Gilray; Sandra Scholes; Fiona Howie; George Caldow; Peter F Nettleton

doi:10.1016/j.vetmic.2007.07.005

Development of a real time reverse transcriptase polymerase chain reaction for the detection of bovine respiratory syncytial virus in clinical samples and its comparison with immunohistochemistry and immunofluorescence antibody testing

Kim Willoughby, Karen Thomson, Madeleine Maley, Janice Gilray, Sandra Scholes, Fiona Howie, George Caldow, Peter F Nettleton

Veterinary Services

Research output: Contribution to journal › Short cummunication › peer-review

Abstract

Bovine respiratory syncytial virus is an agent involved in calf pneumonia complex, a disease of significant economic importance. Accurate diagnosis of the agents involved on farm premises is important when formulating disease control measures, including vaccination. We have developed a real time reverse transcriptase polymerase chain reaction (rtRT-PCR) and compared it with the diagnostic tests currently available in the United Kingdom: immunohistochemistry (IHC) and immunofluorescence antibody test (IFAT). The rtRT-PCR had a detection limit of 10 gene copies and was 96% efficient. Recent UK isolates and clinical samples were tested; the rtRT-PCR was more sensitive than both conventional tests.

Original language	English
Pages (from-to)	264-70
Number of pages	7
Journal	Veterinary Microbiology
Volume	126
Issue number	1-3
DOIs	https://doi.org/10.1016/j.vetmic.2007.07.005
Publication status	Print publication - 1 Jan 2008

Keywords

Animals
Cattle
Cattle Diseases/diagnosis
Fluorescent Antibody Technique, Direct/veterinary
Immunohistochemistry/veterinary
Respiratory Syncytial Virus Infections/diagnosis
Respiratory Syncytial Virus, Bovine/genetics
Reverse Transcriptase Polymerase Chain Reaction/veterinary

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Willoughby, K., Thomson, K., Maley, M., Gilray, J., Scholes, S., Howie, F., Caldow, G., & Nettleton, P. F. (2008). Development of a real time reverse transcriptase polymerase chain reaction for the detection of bovine respiratory syncytial virus in clinical samples and its comparison with immunohistochemistry and immunofluorescence antibody testing. Veterinary Microbiology, 126(1-3), 264-70. https://doi.org/10.1016/j.vetmic.2007.07.005

Willoughby, Kim ; Thomson, Karen ; Maley, Madeleine ; Gilray, Janice ; Scholes, Sandra ; Howie, Fiona ; Caldow, George ; Nettleton, Peter F. / Development of a real time reverse transcriptase polymerase chain reaction for the detection of bovine respiratory syncytial virus in clinical samples and its comparison with immunohistochemistry and immunofluorescence antibody testing. In: Veterinary Microbiology. 2008 ; Vol. 126, No. 1-3. pp. 264-70.

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abstract = "Bovine respiratory syncytial virus is an agent involved in calf pneumonia complex, a disease of significant economic importance. Accurate diagnosis of the agents involved on farm premises is important when formulating disease control measures, including vaccination. We have developed a real time reverse transcriptase polymerase chain reaction (rtRT-PCR) and compared it with the diagnostic tests currently available in the United Kingdom: immunohistochemistry (IHC) and immunofluorescence antibody test (IFAT). The rtRT-PCR had a detection limit of 10 gene copies and was 96% efficient. Recent UK isolates and clinical samples were tested; the rtRT-PCR was more sensitive than both conventional tests.",

keywords = "Animals, Cattle, Cattle Diseases/diagnosis, Fluorescent Antibody Technique, Direct/veterinary, Immunohistochemistry/veterinary, Respiratory Syncytial Virus Infections/diagnosis, Respiratory Syncytial Virus, Bovine/genetics, Reverse Transcriptase Polymerase Chain Reaction/veterinary",

author = "Kim Willoughby and Karen Thomson and Madeleine Maley and Janice Gilray and Sandra Scholes and Fiona Howie and George Caldow and Nettleton, {Peter F}",

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Willoughby, K, Thomson, K, Maley, M, Gilray, J, Scholes, S, Howie, F, Caldow, G & Nettleton, PF 2008, 'Development of a real time reverse transcriptase polymerase chain reaction for the detection of bovine respiratory syncytial virus in clinical samples and its comparison with immunohistochemistry and immunofluorescence antibody testing', Veterinary Microbiology, vol. 126, no. 1-3, pp. 264-70. https://doi.org/10.1016/j.vetmic.2007.07.005

Development of a real time reverse transcriptase polymerase chain reaction for the detection of bovine respiratory syncytial virus in clinical samples and its comparison with immunohistochemistry and immunofluorescence antibody testing. / Willoughby, Kim; Thomson, Karen; Maley, Madeleine; Gilray, Janice; Scholes, Sandra; Howie, Fiona; Caldow, George; Nettleton, Peter F.

In: Veterinary Microbiology, Vol. 126, No. 1-3, 01.01.2008, p. 264-70.

Research output: Contribution to journal › Short cummunication › peer-review

TY - JOUR

T1 - Development of a real time reverse transcriptase polymerase chain reaction for the detection of bovine respiratory syncytial virus in clinical samples and its comparison with immunohistochemistry and immunofluorescence antibody testing

AU - Willoughby, Kim

AU - Thomson, Karen

AU - Maley, Madeleine

AU - Gilray, Janice

AU - Scholes, Sandra

AU - Howie, Fiona

AU - Caldow, George

AU - Nettleton, Peter F

PY - 2008/1/1

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N2 - Bovine respiratory syncytial virus is an agent involved in calf pneumonia complex, a disease of significant economic importance. Accurate diagnosis of the agents involved on farm premises is important when formulating disease control measures, including vaccination. We have developed a real time reverse transcriptase polymerase chain reaction (rtRT-PCR) and compared it with the diagnostic tests currently available in the United Kingdom: immunohistochemistry (IHC) and immunofluorescence antibody test (IFAT). The rtRT-PCR had a detection limit of 10 gene copies and was 96% efficient. Recent UK isolates and clinical samples were tested; the rtRT-PCR was more sensitive than both conventional tests.

AB - Bovine respiratory syncytial virus is an agent involved in calf pneumonia complex, a disease of significant economic importance. Accurate diagnosis of the agents involved on farm premises is important when formulating disease control measures, including vaccination. We have developed a real time reverse transcriptase polymerase chain reaction (rtRT-PCR) and compared it with the diagnostic tests currently available in the United Kingdom: immunohistochemistry (IHC) and immunofluorescence antibody test (IFAT). The rtRT-PCR had a detection limit of 10 gene copies and was 96% efficient. Recent UK isolates and clinical samples were tested; the rtRT-PCR was more sensitive than both conventional tests.

KW - Animals

KW - Cattle

KW - Cattle Diseases/diagnosis

KW - Fluorescent Antibody Technique, Direct/veterinary

KW - Immunohistochemistry/veterinary

KW - Respiratory Syncytial Virus Infections/diagnosis

KW - Respiratory Syncytial Virus, Bovine/genetics

KW - Reverse Transcriptase Polymerase Chain Reaction/veterinary

U2 - 10.1016/j.vetmic.2007.07.005

DO - 10.1016/j.vetmic.2007.07.005

M3 - Short cummunication

C2 - 17709212

VL - 126

SP - 264

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JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 1-3

ER -

Willoughby K, Thomson K, Maley M, Gilray J, Scholes S, Howie F et al. Development of a real time reverse transcriptase polymerase chain reaction for the detection of bovine respiratory syncytial virus in clinical samples and its comparison with immunohistochemistry and immunofluorescence antibody testing. Veterinary Microbiology. 2008 Jan 1;126(1-3):264-70. https://doi.org/10.1016/j.vetmic.2007.07.005