Development of a real time reverse transcriptase polymerase chain reaction for the detection of bovine respiratory syncytial virus in clinical samples and its comparison with immunohistochemistry and immunofluorescence antibody testing

Kim Willoughby, Karen Thomson, Madeleine Maley, Janice Gilray, Sandra Scholes, Fiona Howie, George Caldow, Peter F Nettleton

Research output: Contribution to journalShort cummunication peer-review

Abstract

Bovine respiratory syncytial virus is an agent involved in calf pneumonia complex, a disease of significant economic importance. Accurate diagnosis of the agents involved on farm premises is important when formulating disease control measures, including vaccination. We have developed a real time reverse transcriptase polymerase chain reaction (rtRT-PCR) and compared it with the diagnostic tests currently available in the United Kingdom: immunohistochemistry (IHC) and immunofluorescence antibody test (IFAT). The rtRT-PCR had a detection limit of 10 gene copies and was 96% efficient. Recent UK isolates and clinical samples were tested; the rtRT-PCR was more sensitive than both conventional tests.

Original languageEnglish
Pages (from-to)264-70
Number of pages7
JournalVeterinary Microbiology
Volume126
Issue number1-3
DOIs
Publication statusPrint publication - 1 Jan 2008

Keywords

  • Animals
  • Cattle
  • Cattle Diseases/diagnosis
  • Fluorescent Antibody Technique, Direct/veterinary
  • Immunohistochemistry/veterinary
  • Respiratory Syncytial Virus Infections/diagnosis
  • Respiratory Syncytial Virus, Bovine/genetics
  • Reverse Transcriptase Polymerase Chain Reaction/veterinary

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