Development of paper-based DNA sensor for detection of O. tsutsugamushi using sustainable GQDs@AuNPs nanocomposite

Deepak Kala, Tarun Kumar Sharma, Shagun Gupta, Reena V. Saini, Adesh K. Saini, Walaa F. Alsanie, Vijay Kumar Thakur, Ankur Kaushal*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The graphene quantum dots (GQDs) was synthesized using potato starch and water by hydrothermal method and further used for reduction of tetracholoroauric acid to form graphene quantum dots-gold (GQDs@AuNPs) nanocomposite. The GQDs/GQDs@AuNPs were analyzed using FTIR, UV–Vis, Flourometry and HR-TEM. The synthesized GQDs@AuNPs were further used for fabrication of cost-effective screen-printed paper electrode (SPPE) based DNA sensor for the detection of O. tsutsugamushi using htrA gene specific 5′NH2 linked DNA probe. Modification of SPPE using GQDs@AuNPs nanocomposite and ssDNA probe was monitored using EIS, FTIR, FE-SEM and AFM. The sensor detection limit (LOD) was assessed as 0.002 ng/μl from the standard calibration curve with the correlation coefficient, R2 = 0.993. The sensitivity of the DNA sensor was calculated as 7700 μA/cm2/ng for ssGDNA of O. tsutsugamushi using cyclic voltammetry. The sensor validation was done using scrub typhus patient's blood DNA samples. The sensor showed good storage stability at 4 °C for six months with just a loss of 12% of the initial current values. The SPPE/DNA sensor developed is very specific, sensitive, stable and detects O. tsutsugamushi in less time.

Original languageEnglish
Article number134428
JournalChemosphere
Volume300
Early online date5 Apr 2022
DOIs
Publication statusFirst published - 5 Apr 2022

Keywords

  • DNA sensor
  • GQDs
  • GQDs@AuNPs nanocomposite
  • Screen printed paper electrode
  • Scrub typhus

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