Differential immune phenotypes in human monocytes induced by non-host-adapted salmonella enterica serovar choleraesuis and host-adapted S. Typhimurium

Hiba Ibrahim, Basim Askar, Scott Hulme, Peter Neilson, Paul Barrow, Neil Foster*

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We studied the effects of two Salmonella enterica serovar Typhimurium (host-adapted) strains (14028 and 4/74) and three S. Choleraesuis (non-host-adapted) strains (A50, A45, and B195) in human monocytes between 2 and 24 h postinfection (p.i.) to investigate whether differences in immune response may explain the much higher prevalence of sepsis in individuals infected with S. Choleraesuis. Both serovars significantly increased the production of cytokines associated with acute sepsis (tumor necrosis factor alpha [TNF-α], interleukinβ[IL-β], and IL-6), but temporal differences occurred between these serovars and between different S. Choleraesuis strains. Generally, all S. Choleraesuis strains induced significantly higher production of inflammatory cytokines than S. Typhimurium strains (P< 0.01 to 0.05). All S. Choleraesuis strains very significantly increased IL-10 production by monocytes at 6 and 24 h p.i. in comparison to S. Typhimurium strains (P < 0.01). In addition, ~80% of monocytes were viable at 24 h p.i. with S. Choleraesuis A50, compared to only ~40% following S. Typhimurium infection. Using S. Typhimurium 14028 and S. Choleraesuis A50 as examples of these two serovars, we also showed differential expression of genes within the Janus tyrosine kinase (JAK) and signal transducer and activator of transcription (STAT) (JAK/STAT) pathway via quantitative PCR (qPCR) microarray analysis. High serum IL-10 concentration and monocyte survival have been reported as markers of the development of human sepsis. We therefore conclude that high production of IL-10 by monocytes may, in part, explain the greater propensity for S. Choleraesuis to induce human sepsis and that this may be greater in strains such as A50, which induces both high IL-10 production and monocyte survival.

Original languageEnglish
Article numbere00509-18
JournalInfection and Immunity
Volume86
Issue number10
Early online date21 Sep 2018
DOIs
Publication statusFirst published - 21 Sep 2018
Externally publishedYes

Keywords

  • Human
  • Monocyte
  • Salmonella
  • Sepsis

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